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Current status of the role of Cl− ion in the oxygen-evolving complex

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Abstract

This minireview summarizes the current state of knowledge concerning the role of Cl− in the oxygen-evolving complex (OEC) of photosystem II (PSII). The model that proposes that Cl− is a Mn ligand is discussed in light of more recent work. Studies of Cl− specificity, stoichiometry, kinetics, and retention by extrinsic polypeptides are discussed, as are the results that fail to detect Cl− ligation to Mn and results that show a lack of a requirement for Cl− in PSII-catalyzed H2O oxidation. Mutagenesis experiments in cyanobacteria and higher plants that produce evidence for a correlation between Cl− retention and stable interactions among intrinsic and extrinsic polypeptides are summarized, and spectroscopic data on the interaction between PSII and Cl− are discussed. Lastly, the question of the site of Cl− action in PSII is discussed in connection with the current crystal structures of the enzyme.

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Abbreviations

EGTA:

Ethylene glycol bis(2-aminoethyl ether)-N,N,N′N′-tetraacetic acid

EPR:

Electron paramagnetic resonance

ESEEM:

Electron spin-echo envelope modulation

EXAFS:

Extended X-ray absorption fine structure

FTIR:

Fourier transform infrared spectroscopy

OEC:

Oxygen-evolving complex

PS:

Photo system

PsbO:

33 kDa protein

PsbP:

23 kDa protein

PsbQ:

17 kDa protein

PsbV:

Cyt 550

PsbU:

12 kDa protein

NMR:

Nuclear magnetic resonance

XANES:

X-ray absorption near edge structure

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Acknowledgements

The authors acknowledge support from the National Science Foundation (MCB0110455). CFY acknowledges a number of interesting and fruitful discussions with Govindjee, over more years than he (or Govindjee) would care to admit.

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  1. Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, 48109-1048, USA

    Hana Popelková & Charles F. Yocum

  2. Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109-1048, USA

    Charles F. Yocum

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  1. Hana Popelková
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Correspondence to Charles F. Yocum.

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Popelková, H., Yocum, C.F. Current status of the role of Cl− ion in the oxygen-evolving complex. Photosynth Res 93, 111–121 (2007). https://doi.org/10.1007/s11120-006-9121-5

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