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The Overexpression of Solanum nigrum Osmotin (SnOLP) Boosts Drought Response Pathways in Soybean

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Abstract

Environmental stresses are responsible for limiting soybean yield. To mitigate the impacts generated by water deficit, molecular biology tools are being used to develop genetically modified plants. Previous studies showed that two independent events (B1 and B3) of soybean transgenic plants expressing a Solanum nigrum osmotin (SnOLP) had an increment in drought tolerance. The present study aims to investigate the modulated pathways that results in the drought tolerance promoted by osmotin overexpression in soybean. Transgenic and non-transgenic (NT) plants in the vegetative stage were submitted to water deficit by irrigation suppression for seven days. Control plants were kept irrigated. Physiological variables were monitored and confirmed that the transgenic plants present better performance when compared to the NT plants. The total RNA extracted from leaves was sequenced and data was normalized by DESeq2. A total of 2044 and 1505 differentially expressed genes (DEGs) were identified in B1 and B3 events, respectively. Regarding the B1 event, 769 genes were upregulated and 1275 downregulated. For B3, 541 genes were upregulated and 964 genes were downregulated. Excluding common differentially expressed genes (DEGs) between transgenic and non-transgenic (NT) plants yielded 395 upregulated and 234 downregulated genes, which were shared by B1 and B3 events. The metabolic pathways and gene ontology categories identified are known to be involved in plant responses to drought. Hormonal, photosynthetic, carbohydrate and amino acid metabolism, reactive oxygen species, and post-translational modifications pathways were significantly modulated in transgenic plants. Altogether, the results suggest that osmotin promotes tolerance through an increment in the plant responses elicited by drought.

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Acknowledgements

This research was funded by MCTI/CNPq/CAPES/FAPs no. 16/2014-National Institute of Science and Technology (INCT) in Biotech Assets Applied to Drought and Pests of Relevant Crops to Agrobusiness [88887.136360/2017-00-465480/2014-4].

Funding

This work was supported by MCTI/CNPq/CAPES/FAPs no. 16/2014-National Institute of Science and Technology (INCT) in Biotech Assets Applied to Drought and Pests of Relevant Crops to Agrobusiness, [88887.136360/2017-00-465480/2014-4].

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Authors and Affiliations

  1. Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

    Luisa Abruzzi de Oliveira Busatto, Fernanda Lazzarotto & Christian Bredemeier

  2. Programa de Pós-Graduação em Genética e Biologia Molecular, (PPGBM), Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500 – Prédio 43312, sala 212D, CEP:91501-970, Porto Alegre, RS, 15053, Brazil

    Luisa Abruzzi de Oliveira Busatto, Lariane Frâncio, Fernanda Lazzarotto, Giulia Ramos Faillace, Ricardo Luís Mayer Weber & Maria Helena Bodanese Zanettini

  3. Escuela de Medicina, Universidad Científica del Sur, Lima, 15067, Peru

    Frank Guzman

  4. Instituto Rio Grandense do Arroz (IRGA), Porto Alegre, 90220-007, Brazil

    Débora Favero

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  1. Luisa Abruzzi de Oliveira Busatto
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  9. Maria Helena Bodanese Zanettini
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Contributions

LAOB, LF, CB and MHBZ conceived the study; MHBZ acquired funding and resources for the study, Data analysis done by LAOB, LF, FL, FG, GRF, DF; LAOB, LF, FL, RLMW, CB and MHBZ interpreted the results; and LAOB, FL, MHBZ wrote the manuscript.

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Correspondence to Maria Helena Bodanese Zanettini.

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Key Message

• Soybean overexpressing SnOLP performed better under drought

• SnOLP expression helps plants to retain water and to increase photosynthetic capacity under drought stress

• Gene ontology (GO) analysis identified enriched terms associated with various biological processes, notably including responses to hydrogen peroxide, salt stress, and protein folding among upregulated genes, and borate transmembrane transport and photosynthetic electron transport among downregulated genes

• Shared DEGs between B1 and B3 transgenic events were annotated for 56 metabolic pathways, encompassing various aspects of plant metabolism and stress response

• Hormonal, photosynthetic, carbohydrate and amino acid metabolism, reactive oxygen species, and post-translational modifications pathways were significantly modulated in transgenic plants

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de Oliveira Busatto, L.A., Frâncio, L., Lazzarotto, F. et al. The Overexpression of Solanum nigrum Osmotin (SnOLP) Boosts Drought Response Pathways in Soybean. Plant Mol Biol Rep (2024). https://doi.org/10.1007/s11105-024-01452-7

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