Abstract
Lablab is a regionally important multipurpose legume crop used for human consumption, animal feed, and soil conservation. Despite these qualities, the potential value of this crop has not been fully utilized, and very little research attention has been given to it. The main objective of the study was molecular genetic diversity analysis of Lablab collections using 15 SSR markers. The molecular genetic diversity study of 91 Lablab collections revealed a total of 225 alleles with an average of 14.80 alleles per locus. All markers across the entire population were found to be highly polymorphic and informative with PIC values ranging from 0.78 to 0.92 with a mean value of 0.85. The average expected heterozygosity and gene diversity were 0.75 and 0.86 respectively, indicating a high level of diversity. Analysis of molecular variance showed that 94% of the total genetic variation was attributed to within populations, while only 6% was attributed to among populations. The fixation index value (0.061) recorded indicates the presence of moderate population differentiation as a result of high gene flow (Nm = 3.820) among populations. Due to high gene flow, Cluster, PCoA, and structure analysis did not exactly categorize the populations into genetic groups corresponding to their geographic origin. The observed relatively higher genetic diversity in Konso and West Wellega populations among the eight populations indicates that these areas could be considered hot-spots for genetic diversity and possible germplasm evaluation. Generally, genetic diversity obtained from this study provides inputs for Lablab conservation and improvement in Ethiopia.
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No datasets were generated or analysed during the current study.
References
Amkul K, Sookbang JM, Somta P (2021) Genetic diversity and structure of landrace of Lablab (Lablab purpureus (L.) sweet) cultivars in Thailand revealed by SSR markers. Breed Sci 71:176–183. https://doi.org/10.1270/jsbbs.20074
Chang Y, Liu H, Liu M et al (2018) The draft genomes of five agriculturally important African orphan crops. Gigascience 8:1–16. https://doi.org/10.1093/gigascience/giy152
D’Souza MR, Devaraj VR (2010) Biochemical responses of Hyacinth bean (Lablab purpureus) to salinity stress. Acta Physiol Plant 32:341–353. https://doi.org/10.1007/s11738-009-0412-2
Diego S, Jolla L (1987) A Multispecies Approach to the Analysis of 41:385–400
Earl DA, von Holdt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361. https://doi.org/10.1007/s12686-011-9548-7
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
Galluzzi G, Halewood M, López Noriega I, Vernooy R (2016) Twenty-five years of international exchanges of plant genetic resources facilitated by the CGIAR genebanks: a case study in international interdependence. Biodivers Conserv 25:1421–1446. https://doi.org/10.1007/s10531-016-1109-7
Gilbert JE, Lewis RV, Wilkinson MJ, Caligari PDS (1999) Developing an appropriate strategy to assess genetic variability in plant germplasm collections. Theor Appl Genet 98:1125–1131. https://doi.org/10.1007/s001220051176
Habib HM, Theuri SW, Kheadr EE, Mohamed FE (2017) Functional, bioactive, biochemical, and physicochemical properties of the Dolichos Lablab bean. Food Funct 8:872–880. https://doi.org/10.1039/c6fo01162d
Hirpara DG, Gajera HP (2018) Molecular heterozygosity and genetic exploitations of Trichoderma inter-fusants enhancing tolerance to fungicides and mycoparasitism against Sclerotium rolfsii Sacc. Infect Genet Evol 66:26–36. https://doi.org/10.1016/j.meegid.2018.09.005
Kalinowski ST (2005) HP-RARE 1.0: a computer program for performing rarefaction on measures of allelic richness. Mol Ecol Notes 5:187–189. https://doi.org/10.1111/j.1471-8286.2004.00845.x
Kamau EM, Kinyua MG, Waturu CN, Kiplagat O, Wanjala BW, Kariba RK, David RK (2021) Diversity and population structure of local and exotic Lablab purpureus accessions in Kenya as revealed by microsatellite markers. Glob J Mol Biol 3(8):1–16
Kamotho GN, Kinyua MG, Muasya RM et al (2016) Assessment of genetic diversity of Kenyan dolichos bean ( Lablab purpureus l. Sweet ) using simple sequence repeat ( SSR ) markers. Int J Agric Environ Bioresearch 1:26–43
Keerthi CM, Ramesh S, Byregowda M, Vaijayanthi PV (2018) Simple sequence repeat (SSR) marker assay-based genetic diversity among dolichos bean (Lablab purpureus L. Sweet) advanced breeding lines differing for productivity per se traits. Int J Curr Microbiol Appl Sci 7:3736–3744. https://doi.org/10.20546/ijcmas.2018.705.433
Keneni G, Bekele E, Imtiaz M et al (2012) Genetic diversity and population structure of Ethiopian chickpea (Cicer arietinum L.) germplasm accessions from different geographical origins as revealed by microsatellite markers. Plant Mol Biol Report 30:654–665. https://doi.org/10.1007/s11105-011-0374-6
Kimani EN, Wachira FG, Kinyua M (2012) Molecular diversity of Kenyan Lablab bean (Lablab purpureus (L.) Sweet) accessions using amplified fragment length polymorphism markers. Am J Plant Sci 03:313–321. https://doi.org/10.4236/ajps.2012.33037
Kongjaimun A, Takahashi Y, Yoshioka Y, Tomooka N, Mongkol R, Somta P (2022) Molecular analysis of genetic diversity and structure of the Lablab (Lablab purpureus (L.) Sweet) gene pool reveals two independent routes of domestication. Plants 12(1):57. https://doi.org/10.3390/plants12010057
Kopelman NM, Mayzel J, Jakobsson M et al (2015) Clumpak: a program for identifying clustering modes and packaging population structure inferences across K. Mol Ecol Resour 15:1179–1191. https://doi.org/10.1111/1755-0998.12387
Kukade SA, Tidke JA (2014) Reproductive biology of Dolichos lablab L. (Fabaceae). Indian J Plant Sci 3:22–25
Liu CJ (1996) Genetic diversity and relationships among Lablab purpureus genotypes evaluated using RAPD as markers
Liu K, Muse SV (2005) PowerMaker: an integrated analysis environment for genetic maker analysis. Bioinformatics 21:2128–2129. https://doi.org/10.1093/bioinformatics/bti282
Liu YM, Shahed-Al-Mahmud M, Chen X et al (2020) A carbohydrate-binding protein from the edible Lablab beans effectively blocks the infections of influenza viruses and SARS-CoV-2. Cell Rep 32:108016. https://doi.org/10.1016/j.celrep.2020.108016
Maass BL (2016) Origin, domestication and global dispersal of Lablab purpureus (L.) Sweet (Fabaceae): current understanding. Legum Perspect 5–8
Maass BL, Jamnadass RH, Hanson J, Pengelly BC (2005) Determining sources of diversity in cultivated and wild Lablab purpureus related to provenance of germplasm by using amplified fragment length polymorphism. Genet Resour Crop Evol 52:683–695. https://doi.org/10.1007/s10722-003-6019-3
Maass BL, Knox MR, Venkatesha SC et al (2010) Lablab purpureus-a crop lost for Africa? Trop Plant Biol 3:123–135. https://doi.org/10.1007/s12042-010-9046-1
Maass BL, Robotham O, Chapman MA (2017) Evidence for two domestication events of hyacinth bean (Lablab purpureus (L.) Sweet): a comparative analysis of population genetic data. Genet Resour Crop Evol 64:1221–1230. https://doi.org/10.1007/s10722-016-0431-y
Maass BL, Chapman MA (2022) The Lablab genome: Recent advances and future perspectives. Chapter 13, pp. 229-253 in: Chapman MA (ed) Underutilised crop genomes. Series: Compendium of Plant Genomes. Springer, Cham, Germany. https://doi.org/10.1007/978-3-031-00848-1_13
Minde JJ, Venkataramana PB, Matemu AO (2021) Dolichos Lablab-an underutilized crop with future potentials for food and nutrition security: a review. Crit Rev Food Sci Nutr 61:2249–2261. https://doi.org/10.1080/10408398.2020.1775173
Naeem M, Shabbir A, Ansari AA et al (2020) Hyacinth bean (Lablab purpureus L.) – an underutilised crop with future potential. Sci Hortic (Amsterdam) 272. https://doi.org/10.1016/j.scienta.2020.109551
Nord A, Miller NR, Mariki W et al (2020) Investigating the diverse potential of a multipurpose legume, Lablab purpureus (L.) Sweet, for smallholder production in East Africa. PLoS One 15:. https://doi.org/10.1371/journal.pone.0227739
Pavel AB, Vasile CI (2012) PyElph - a software tool for gel images analysis and phylogenetics. BMC Bioinformatics 13:. https://doi.org/10.1186/1471-2105-13-9
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Pengelly BC, Maass BL (2001) Lablab purpureus (L.) Sweet - diversity, potential use and determination of a core collection of this multi-purpose tropical legume. Genet Resour Crop Evol 48:261–272. https://doi.org/10.1023/A:1011286111384
Perrier X, Jacquemoud-Collet JP (2006) DARwin software https://DARwin.cirad.fr
Powell’ W, Morgante2 M, Andre3 C et al (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Kluwrr Academic Publishers
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959. https://doi.org/10.1093/genetics/155.2.945
Rangaiah D V, Dsouza M (2016) Hyacinth bean (Lablab purpureus): an adept adaptor to adverse environments
Robotham O, Chapman M (2017) Population genetic analysis of hyacinth bean (Lablab purpureus (L.) Sweet, Leguminosae) indicates an East African origin and variation in drought tolerance. Genet Resour Crop Evol 64(441):139–148. https://doi.org/10.1007/s10722-015-0339-y, https://doi.org/10.1007/s10722-015-0356-x
She CW, Jiang XH (2015) Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes. Czech J Genet Plant Breed 51:110–116. https://doi.org/10.17221/32/2015-CJGPB
Slatkin M (1987) Gene flow and the geographic structure of natural populations. Science 236(80):787–792. https://doi.org/10.1126/science.3576198
DARTs (2019) Plant DNA extraction protocol for DArT. 2–3
Tesfaye A (2007) Plant diversity in Western Ethiopia: ecology , ethnobotany and conservation. PhD Thesis
Wang ML, Morris JB, Barkley NA et al (2007) Evaluation of genetic diversity of the USDA Lablab purpureus germplasm collection using simple sequence repeat markers. J Hortic Sci Biotechnol 82:571–578. https://doi.org/10.1080/14620316.2007.11512275
Weldeyesus G (2017) Forage productivity system evaluation through station screening and intercropping of Lablab forage legume with maize under Irrigated lands of smallholder farmers. African J Agric Res 12:1841–1847. https://doi.org/10.5897/ajar2016.11989
Westphal E (1974) Pulses in Ethiopia, their taxonomy and agricultural significance. Kew Bull 30:590. https://doi.org/10.2307/4103104
Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354. https://doi.org/10.1111/j.1469-1809.1949.tb02451.x
Zhang G, Xu S, Mao W et al (2013) Development of EST-SSR markers to study genetic diversity in hyacinth bean (Lablab purpureus L.). Plant Omics 6:295–301
Acknowledgements
The authors would like to acknowledge the Ethiopian Institute of Agricultural Research, McKnight Foundation Collaborative Crop Research Program through the Ethiopian Legume Diversity Project implemented by the Department of Plant Biology and Biodiversity Management, and the Addis Ababa University, Institute of Biotechnology of the Addis Ababa University, for their financial and other associated supports for this research. We are also thankful to the Ethiopian Biodiversity Institute (EBI) and Bako Agricultural Research Center for providing Lablab germplasm used in this study. BL Maass is acknowledged for critical comments on previous versions of the manuscript.
Funding
This research was funded by the Ethiopian Institute of Agricultural Research and the McKnight Foundation Collaborative Crop Research Program through its financial support made to the Ethiopian Legume Diversity Project.
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ZA, TF, and STW contributed to the study conception and design. Material preparation, data collection, and analysis were performed by STW and TD. The first draft of the manuscript was written by STW, TF, ZA, and TD. All authors read and approved the final manuscript.
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Workneh, S.T., Feyissa, T., Asfaw, Z. et al. Genetic Diversity and Population Structure of Lablab (Lablab purpureus L. Sweet) Accessions from Ethiopia Using SSR Markers. Plant Mol Biol Rep (2024). https://doi.org/10.1007/s11105-024-01447-4
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DOI: https://doi.org/10.1007/s11105-024-01447-4