Abstract
Camelina (Camelina sativa L. Crantz) is a short-season oilseed crop of the Brassicaceae family that consists of both summer and winter annual biotypes. Winter biotypes require non-freezing cold conditions for acquiring freezing tolerance (cold acclimation) and floral initiation (vernalization). Transcriptome profiles of a summer (CO46) biotype with poor freezing tolerance after acclimation and a winter (Joelle) biotype with excellent freezing tolerance after acclimation were compared prior to and after an 8-week cold treatment to identify key molecular pathways and genes responsive to cold acclimation and vernalization and potentially associated with freezing tolerance. Gene-set enrichment analyses identified AraCyc pathways involved in photosynthesis and lipid and hormone biosynthesis that were different between the two biotypes. Sub-network enrichment analyses identified hubs of molecular networks such as circadian clock, flowering, and hormone and stress responsive genes that were likely involved in vernalization but may also overlap with cold-induced freezing tolerance. A microRNA involved in floral initiation (MIR172A) was identified as a central hub for microRNA targets among upregulated genes for Joelle post-acclimation. Combined results are generally consistent with many previously identified molecular pathways and genes acting together to control vernalization, cold acclimation, and freezing tolerance. Our research provides new insights into the regulation of cold acclimation and molecular genetic mechanisms underlying cold tolerance and floral induction for the winter biotype Joelle.
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Acknowledgements
The authors wish to thank Brant Bigger, Cheryl Huckle, and Wayne Sargent for their technical assistance during this study.
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JVA conceived and designed the study. JVA, MD, and DPH analyzed the RNAseq data; JVA, MD, WSC, and HW analyzed the Pathway Studio results; WSC and HW wrote the initial draft; and WSC, HW, JVA, MD, and DPH revised and approved the final draft.
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Key message
• Transcriptome profiles for winter and summer biotypes of Camelina provided evidence for induction of freezing tolerance and vernalization.
• Molecular pathways involved in photosynthesis; fatty acid and hormone biosynthesis; differentially regulated genes such as VIN3, FLC, LHY, and LEA14; and microRNA MIR172A have various roles in vernalization, cold acclimation, and potentially freezing tolerance.
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11105_2021_1324_MOESM5_ESM.xlsx
Supplementary file5 (XLSX 43 KB). Additional file 5: Table S5. Differentially-expressed genes associated with cold acclimation and freezing tolerance.
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Wang, H., Doğramacı, M., Anderson, J.V. et al. Transcript Profiles Differentiate Cold Acclimation-Induced Processes in a Summer and Winter Biotype of Camelina. Plant Mol Biol Rep 40, 359–375 (2022). https://doi.org/10.1007/s11105-021-01324-4
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DOI: https://doi.org/10.1007/s11105-021-01324-4