Abstract
Myrtaceae has many fruit-bearing species of economic and ecological relevance worldwide, but with microsatellite molecular markers (simple sequence repeats, SSR) available for only few of them. Thus, the transferability of SSR is advantageous for characterization, conservation, and breeding of these species. In this work, 158 SSR from Psidium guajava were evaluated for transferability in 18 fruit trees of the genera Eugenia, Campomanesia, Myrciaria, and Syzygium. Of those, 152 SSR transferred to at least 1 and up to 16 species; 47 SSR amplified in more than 10 species, the most conserved; and 13 SSR transferred to 1 or 2 species, demonstrating specificity. A large number of conserved SSR are also present in the genetic map of P. guajava, and may be helpful for synteny and comparative genomic studies. The average of transferability was lower for Syzygium and similar in the other genera. Transferability in the species varied from 20 SSR for Syzygium cumini to 91 for Myrciaria sp.2, being higher on average in the genus Eugenia. Clustering based on the pattern of transferability between species revealed two groups, one composed by Eugenia and Campomanesia and another by Syzygium and Myrciaria, which indicates conserved microsatellite regions within and between genera. This study also allowed selection of the most suitable SSR for each species and genus by amplification quality and study type, thus contributing for a better comprehension of the relationships between the species, taxonomy, structure and genetic diversity, construction of genetic maps, and breeding and conservation programs in the family.
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References
Acuña CV, Fernandez P, Villalba PV, García MN, Hopp HE, Poltri SNM (2012) Discovery, validation, and in silico functional characterization of EST-SSR markers in Eucalyptus globulus. Tree Genet Genomes 8:289–301
Ahmed TA, Al-Qaradawi AY (2009) Molecular phylogeny of Qatari date palm genotypes using simple sequence repeats markers. Biotechnology 8:126–131
Barbará T, Palma-Silva C, Paggi GM, Bered F, Fay MF, Lexer C (2007) Cross-species transfer of nuclear microsatellite markers: potential and limitations. Mol Ecol 16:3759–3767
Barroso GM (1991) Myrtaceae. In: Sistemática de angiospermas do Brasil. UFV, Viçosa-MG, 2:114-126
Barthe S, Gureli F, Barkley NA, Maggia L, Cardi C, Scotti I (2012) Always look on both sides: phylogenetic information conveyed by simple sequence repeat allele sequences. PloS One 7:1–9
Biffin E, Lucas EJ, Craven LA, da Costa IR, Harrington MG, Crisp MD (2010) Evolution of exceptional species richness among lineages of fleshy-fruited Myrtaceae. Ann Bot 106:79–93
Brondani RPV, Brondani C, Tarchini R, Grattapaglia D (1998) Development, characterization and mapping of microsatellite markers in Eucalyptus grandis and E. urophylla. Theor Appl Genet 97:816–827
Brondani RPV, Williams ER, Brondani C, Grattapaglia D (2006) A microsatellite-based consensus linkage map for species of Eucalyptus and a novel set of 230 microsatellite markers for the genus. BMC Plant Biol 6:1–16
Buschiazzo E, Gemmell NJ (2009) Evolutionary and phylogenetic significance of platypus microsatellites conserved in mammalian and other vertebrate genomes. Aust J Zool 57:175–184
Costa IR (2004) Estudos cromossômicos em espécies de Myrtaceae Juss. no sudeste do Brasil. Dissertation, University State of Campinas
Curtu AL, Finkeldey R, Gailing O (2004) Comparative sequencing of a microsatellite locus reveals size homoplasy within and between European oak species (Quercus ssp.). Plant Mol Biol Report 22:339–346
Dickey AM, Hall PM, Shatters Júnior RG, Mckenzie CL (2013) Evolution and homoplasy at the Bem6 microsatellite locus in three sweetpotato whitefly (Bemisia tabaci) cryptic species. BMC Res Notes 6:249
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
England PR, Usher AV, Whelan RJ, Ayre DJ (2002) Microsatellite diversity and genetic structure of fragmented populations of the rare, fire-dependent shrub Grevillea macleayana. Mol Ecol 11:967–977
Ferreira-Ramos R, Accoroni KAG, Rossi A, Guidugli MC, Mestriner MA, Martinez CA, Alzate-Marin AL (2014) Genetic diversity assessment for Eugenia uniflora L., E. pyriformis Cambess, E. brasiliensis Lam. and E. francavilleana O. Berg neotropical tree species (Myrtaceae) with heterologous SSR markers. Genet Resour Crop Evol 61:267–272
Govaerts R, Sobral M, Ashton P, Barrie F, Holst BK, Landrum LR, Matsumoto K, Mazine FF, Nic Lughadha E, Proença CEB, Silva LHS, Wilson PG, Lucas EJ (2008) World checklist of Myrtaceae. Kew Publishing, Royal Botanic Gardens
Grattapaglia D, Vaillancourt RE, Shepherd M, Thumma BR, Foley W, Külheim C, Potts BM, Myburg AA (2012) Progress in Myrtaceae genetics and genomics: Eucalyptus as the pivotal genus. Tree Genet Genomes 8:463–508
Guavamap, Generation and screening of microsatellite markers (SSRs) in Guava (2008). http://www.neiker.net/neiker/guavamap/for1-6a.html. Accessed 24 April 2013
Guo W, Wang W, Zhou B, Zhang W (2006) Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Theor Appl Genet 112:1573–1581
Guyomarc H, Sourdille P, Charmet G, Edwards KJ, Bernard M (2002) Characterization of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat. Theor Appl Genet 104:1164–1172
He X, Wang Y, Li F, Weng Q, Li M, Xu LA, Gan S (2012) Development of 198 novel EST-derived microsatellites in Eucalyptus (Myrtaceae). Am J Bot 99:134–148
Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK (2011) Microsatellite markers: an overview of the recent progress in plants. Euphytica 177:309–334
Lucas EJ, Harris SA, Mazine FF, Belsham SR, Lughadha EMN, Telford A, Gasson PE, Chase MW (2007) Suprageneric phylogenetics of Myrteae, the generically richest tribe in Myrtaceae (Myrtales). Taxon 4:1105–1128
Oliveira EJ, Pádua JG, Zucchi MI, Vencovsky R, Vieira MLC (2006) Origin, evolution and genome distribution of microsatellites. Genet Mol Biol 29:294–307
Ottewell KM, Donnellan SC, Moran GF, Paton DC (2005) Multiplexed microsatellite markers for the genetic analysis of Eucalyptus leucoxylon (Myrtaceae) and their utility for ecological and breeding studies in other Eucalyptus Species. J Hered 96:445–451
Parida SK, Kalia SK, Sunita K, Dalal V, Hemaprabha G, Selvi A, Pandit A, Singh A, Gaikwad K, Sharma TR, Srivastava PS, Singh NK, Mohapatra T (2009) Informative genomic microsatellite markers for efficient genotyping applications in sugarcane. Theor Appl Genet 118:327–338
Rai MK, Phulwaria M, Shekhawat NS (2013) Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species. Mol Biol Rep 40:5067–5071
Ramos R, Ravest G, Méndez MA, Hinrichsen P (2012) Highly polymorphic microsatellite markers for Ugni molinae Turcz., and endemic shrub harboring edible berries from the Southern Cone of South America. Mol Ecol Resour 12:1–8
Risterucci AM, Duval MF, Rohde W, Billotte N (2005) Isolation and characterization of microsatellite loci from Psidium guajava L. Mol Ecol Notes 5:745–748
Rodriguez H, Geistlinger J, Berlyn G, Kahl G, Weising K (2000) Characterization of novel microsatellite loci isolated from the tropical dioecious tree Simarouba amara. Mol Ecol 9:489–504
Santos KL, Welter LJ, Dantas ACM, Guerra MP, Ducroquet JPJ, Nodari RO (2007) Transference of microsatellite markers from Eucalyptus spp to Acca sellowiana and the successful use of this technique in genetic characterization. Genet Mol Biol 30:73–79
Santos KL, Ducroquet JPHJ, Nodari RO (2011) Caracterização genética de populações naturais de goiabeira serrana (Acca sellowiana) com marcadores microssatélites heterólogos. Biotemas 4:75–83
Shepherd M, Kasem S, Lee D, Henry R (2006) Construction of microsatellite linkage maps for Corymbia. Silvae Genet 55:228–238
Sonah H, Deshmukh RK, Sharma A, Singh VP, Gupta DK, Gacche RN, Rana JC, Singh NK, Sharma TR (2011) Genome-wide distribution and organization of microsatellites in plants: an insight into marker development in Brachypodium. PloS One 6:21298
Stàgel A, Portis E, Toppino GL, Rotino LS (2008) Gene-based microsatellite development for mapping and phylogeny studies in eggplant. BMC Genomics 9:1–14
Steane D, Vaillancourt R, Russell J, Powell W, Marshall D, Potts B (2001) Development and characterisation of microsatellite loci in Eucalyptus globule (Myrtaceae). Silvae Genet 50:89–91
Suwabe K, Iketani H, Numone T, Kage T, Hirai M (2002) Isolation and characterization of microsatellites in Brassica rapa L. Theor Appl Genet 104:1092–1098
Swapna M, Sivaraju K, Sharma RK, Singh NK, Mohapatra T (2011) Single-strand conformational polymorphism of EST-SSRs: a potential tool for diversity analysis and varietal identification in sugarcane. Plant Mol Biol Report 29:505–513
Taylor DR, Keller SR (2007) Historical range expansion determines the phylogenetic diversity introduced during contemporary species invasion. Evolution 61:335–345
Varshney RK, Graner A, Sorrells ME (2005) Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23:48–55
Wilson PG, O’Brien M, Gadek PA, Quinn CJ (2001) Myrtaceae revisited: a reassessment of intrafamilial groups. Am J Bot 11:2013–2025
Wilson PG, O’Brien MM, Heslewood MM, Quinn CJ (2005) Relationships within Myrtaceae sensu lato based on a matK phylogeny. Plant Syst Evol 251:3–19
Zucchi MI, Brondani RPV, Pinheiro JB, Brondani C, Vencovsky R (2002) Transferability of microsatellite markers from Eucalyptus spp. to Eugenia dysenterica (Myrtaceae family). Mol Ecol Notes 2:512–513
Zucchi MI, Brondani RPV, Pinheiro JB, Chaves LJ, Coelho ASG, Vencovsky R (2003) Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. Genet Mol Biol 26:449–457
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The authors are grateful to the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil)), the Foundation for Research Support of Espírito Santo (Fundação de Amparo à Pesquisa do Espírito Santo (FAPES, Vitória, ES, Brazil)), and the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil)) for financial support.
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Nogueira, A.M., Ferreira, A. & da Silva Ferreira, M.F. Transferability of Microsatellites from Psidium guajava to Eugenia, Myrciaria, Campomanesia, and Syzygium Species (Myrtaceae). Plant Mol Biol Rep 34, 249–256 (2016). https://doi.org/10.1007/s11105-015-0921-7
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DOI: https://doi.org/10.1007/s11105-015-0921-7