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Enhancement of Cytochrome b 559 Indicates Its Possible Involvement in Long-Term High Light Stress Tolerance in Intertidal Macroalgae

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Abstract

The effects of high light (HL) stress on the photosynthetic proteins of green tide-forming macroalga were studied. The naturally grown Ulva prolifera was collected from an intertidal habitat and subjected to 1000 μE m−2 s−1 HL for 12 h. The thylakoid membrane proteins were subsequently extracted and qualitatively analyzed using LC/MS. Quantitative analysis of the thylakoid membrane proteins showed that both PSII reaction center proteins and PSII chlorophyll antenna proteins were decreased. Comparatively, the main PSI reaction center proteins were well preserved. The cytochrome f subunit was significantly decreased, while the other subunits of cytochrome b 6 /f complex were maintained. These results demonstrated that the HL-induced cytochrome b 559 might play a role in photoacclimation of U. prolifera by involving in cyclic photosynthetic electron transfer (PET) flow around PSII. The LhcSR and PsbS proteins were also identified in U. prolifera, and PsbS was noted to be significantly increased under HL stress. The long-term photoacclimation mechanism of U. prolifera under HL stress might not depend on cyclic PET flow around PSI.

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Acknowledgments

This work was supported by the National Science & Technology Program (2012BAC07B03), the National Natural Science Foundation of China (41176134; 41176137), Science and Technology Strategic Pilot of the Chinese Academy of Sciences (XDA05030401) and Public Science and Technology Research Funds Projects of the Ocean (201205010).

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, 266071, Qingdao, China

    Wenhui Gu, Li Huan & Guangce Wang

  2. University of Chinese Academy of Sciences, 100049, Beijing, China

    Li Huan

  3. College of Marine Science and Engineering, Tianjin University of Science and Technology, 300457, Tianjin, China

    Ruixue Yu & Guanghua Pan

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  1. Wenhui Gu
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  2. Li Huan
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  3. Ruixue Yu
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  4. Guanghua Pan
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  5. Guangce Wang
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Correspondence to Guangce Wang.

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Gu, W., Huan, L., Yu, R. et al. Enhancement of Cytochrome b 559 Indicates Its Possible Involvement in Long-Term High Light Stress Tolerance in Intertidal Macroalgae. Plant Mol Biol Rep 33, 1918–1927 (2015). https://doi.org/10.1007/s11105-015-0885-7

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