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Antisense RhMLO1 Gene Transformation Enhances Resistance to the Powdery Mildew Pathogen in Rosa multiflora

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Abstract

Powdery mildew is one of the most important crop diseases worldwide. Genetic analysis has revealed that mutant alleles of the Mlo gene cause broad-spectrum resistance against pathogens found in cereal. In this study, the possibility of inducing powdery mildew (Podosphaera pannosa) resistance via transgenic technology in the rose was examined. The transgenic lines were confirmed for integration and copy number of the transgenes based on PCR and Southern blots. A clear correlation was found between resistance and the accumulation of silenced RhMLO1, based on real-time fluorescent quantitative PCR and resistance analysis. Compared with the control plants, transgenic roses displayed higher resistance levels. Furthermore, the results indicated that RhMLO1 has a negative role in the rose–powdery mildew pathogen interaction. This demonstrates the potentially viable strategy of rendering the Mlo homologs partially non-functional via a transgenic approach or mutagenesis in order to counter powdery mildew in Rosa. To the best of our knowledge, this study is one of the first attempts at using a Mlo-based resistance strategy to combat powdery mildew in Rosa.

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Acknowledgments

This study was supported by funds from the Ministry of Science and Technology Project of China (No. 2011AA100208), National Natural Science Foundation of China (No. 31160403, No. 31160402 and No. 31360492), Natural Science Foundation of Yunnan (Nos. 2011FB124, 2014FB158, and 2011BB013), and Ministry of Agriculture Project of China (Nos. 200903020 and 2011G17).

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China

    Xianqin Qiu & Manzhu Bao

  2. National Engineering Research Center for Ornamental Horticulture, Yunnan Flower Breeding Key Lab, Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650200, China

    Xianqin Qiu, Qigang Wang, Hao Zhang, Hongying Jian, Ningning Zhou, Cheng Ji, Huijun Yan & Kaixue Tang

Authors
  1. Xianqin Qiu
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  2. Qigang Wang
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  3. Hao Zhang
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  4. Hongying Jian
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  5. Ningning Zhou
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  6. Cheng Ji
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  7. Huijun Yan
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  8. Manzhu Bao
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  9. Kaixue Tang
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Corresponding authors

Correspondence to Manzhu Bao or Kaixue Tang.

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Supplementary Fig. 1

Construction of plant antisense inhibition vector pFGC5941-anti-RhMLO1 (JPEG 56 kb)

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Qiu, X., Wang, Q., Zhang, H. et al. Antisense RhMLO1 Gene Transformation Enhances Resistance to the Powdery Mildew Pathogen in Rosa multiflora . Plant Mol Biol Rep 33, 1659–1665 (2015). https://doi.org/10.1007/s11105-015-0862-1

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