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Genome-Wide Dissection of Arabidopsis and Rice for the Identification and Expression Analysis of Glutathione Peroxidases Reveals Their Stress-Specific and Overlapping Response Patterns

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Abstract

Excessive generation of reactive oxygen species (ROS) due to environmental stresses critically effects plant development and productivity. Plants efficiently detoxify ROS by both non-enzymatic and enzymatic mechanisms. Plant glutathione peroxidases (GPXs) are non-haeme thiol peroxidases that catalyze the reduction of H2O2 (or organic hydroperoxides) to water or the respective alcohols using reduced glutathione or thioredoxin. Genome-wide analysis of the known GPXs from rice and Arabidopsis genomes revealed their gene structure, conserved motifs, localization and tissue-specific and/or organ-specific expression profiles in response to various abiotic stresses. Among the eight genes that encoded GPX proteins from Arabidopsis, AtGPX3 showed two alternate spliced forms that spread over four chromosomes. Five genes encoded for rice GPX proteins, while OsGPX1 showed three spliced variants that were distributed on five chromosomes. Utilizing the publicly available microarray and massively parallel signature sequencing (MPSS) data, the GPXs revealed stress-responsive, tissue-specific and/or organ-specific expression profiles. Presence of important cis-regulatory elements analyzed in the GPX promoter sequences revealed their overlapping or specific responsiveness to different abiotic stresses. Co-expression data of Arabidopsis GPX genes suggested that various protein kinase family members and stress-responsive proteins co-expressed with the GPX proteins. Transcript profile of rice GPX genes by qRT-PCR validated their functional roles in signal transduction and stress pathways. Results revealed that plant GPXs play a crucial role in response to stress and significantly contribute towards their growth and development.

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Acknowledgments

We thank ICGEB for the pre-doctoral fellowship awarded to Tahmina Islam and CSIR for awarding the fellowship to Mrinalini Manna. In addition, we thank the Biocare grant from Department of Biotechnology awarded to Dr. Tanushri Kaul for providing us the funds for accomplishment of the aforesaid research work.

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  1. Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Tahmina Islam, Mrinalini Manna, Tanushri Kaul, Saurabh Pandey, C. Subramanyam Reddy & M. K. Reddy

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  1. Tahmina Islam
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  2. Mrinalini Manna
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  3. Tanushri Kaul
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  4. Saurabh Pandey
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Correspondence to Tanushri Kaul.

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Islam, T., Manna, M., Kaul, T. et al. Genome-Wide Dissection of Arabidopsis and Rice for the Identification and Expression Analysis of Glutathione Peroxidases Reveals Their Stress-Specific and Overlapping Response Patterns. Plant Mol Biol Rep 33, 1413–1427 (2015). https://doi.org/10.1007/s11105-014-0846-6

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