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Apple 1-Aminocyclopropane-1-Carboxylic Acid Synthase Genes, MdACS1 and MdACS3a, are Expressed in Different Systems of Ethylene Biosynthesis

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Abstract

1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is one of the key regulatory enzymes involved in the synthesis of ethylene. Climacteric fruit ripening is accompanied by increased ethylene production, in which ethylene biosynthesis is changed from system 1 to system 2. In apple, at least four members of the ACS gene family have been identified, two of which, MdACS1 and MdACS3a, have been studied extensively due to their specific expression in fruit tissue. However, the regulatory role of MdACS1 and MdACS3a in the ethylene biosynthesis system is unknown. Here we addressed this issue by investigating ACS expression in ripening apple fruits. Expression analysis in ‘Golden Delicious’ and ‘Red Fuji’ fruits, in combination with treatments of 1-MCP (1-methylcyclopropene, an ethylene inhibitor) and Ethephon (an ethylene releaser) has demonstrated that MdACS3a and MdACS1operate in system 1 and system 2 ethylene biosynthesis, respectively.

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Acknowledgments

We thank Dr. Zhi Liu from the Liaoning Institute of Pomology for kindly providing the plant materials. This work was supported by the Foundation for Youth Researcher of Shenyang Agricultural University (20111002).

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Authors and Affiliations

  1. Fruit Development and Metabolic Biology Laboratory, College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, 110866, People’s Republic of China

    Dongmei Tan & Aide Wang

  2. Laboratory of Fruit Cell and Molecular Breeding, College of Agronomy and Bio-tech, China Agricultural University, Beijing, 100193, People’s Republic of China

    Tianzhong Li

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  1. Dongmei Tan
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  2. Tianzhong Li
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  3. Aide Wang
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Correspondence to Aide Wang.

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Tan, D., Li, T. & Wang, A. Apple 1-Aminocyclopropane-1-Carboxylic Acid Synthase Genes, MdACS1 and MdACS3a, are Expressed in Different Systems of Ethylene Biosynthesis. Plant Mol Biol Rep 31, 204–209 (2013). https://doi.org/10.1007/s11105-012-0490-y

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