Abstract
Jatropha (Jatropha curcas) and castor bean (Ricinus communis) possess several taxonomic similarities, and their seeds contain a high proportion of oil (up to 40%) which has been used in various industrial products, including diesel oil. Thirty-two candidate genes responsible for fatty acid biosynthesis were identified in the castor bean genome sequence. Testing of 48 primer pairs from candidate gene regions, including 12 SSRs from castor bean on 54 genotypes of J. curcas, 65% amplified successfully on Jatropha out of which 20% showed polymorphisms. Jatropha genotypes, categorized for oil content, were used in association analysis of candidate gene regions with high oil content. One marker–trait association for the oil trait was identified. Stearoyl desaturase amplicon (700 bp) consisting of intron and exon (P = 0.00013) showed association with high oil content in Jatropha genotypes. Sequencing of the 1.3-kb amplicon, including the 700-bp fragment of stearoyl desaturase, which had shown association with the high oil content, revealed SNPs in the exonic region. The SNPs resulted in substitution of leucine with glutamine in the open reading frame of stearoyl desaturase of low oil content genotypes. The molecular marker is expected to be useful in marker-assisted breeding of high oil content genotypes in Jatropha.
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Acknowledgment
The authors are thankful to the Department of Biotechnology, Ministry of Science and Technology, Government of India for providing a research grant to RSC.
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Supplementary Table 1
Primers used to amplify candidate genes for fatty acid biosynthesis in Jatropha (DOCX 42 kb)
Supplementary Table 2
SSRs identified in the candidate genes for fatty acid biosynthesis in castor bean (DOCX 36 kb)
Supplementary Table 3
List of genotypes used in the present study (DOCX 37 kb)
Supplementary Table 4
Annotation of candidate genes for fatty acid biosynthesis in the castor bean genome (DOCX 49 kb)
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Sharma, A., Chauhan, R.S. Identification and Association Analysis of Castor Bean Orthologous Candidate Gene-Based Markers for High Oil Content in Jatropha curcas . Plant Mol Biol Rep 30, 1025–1031 (2012). https://doi.org/10.1007/s11105-011-0408-0
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DOI: https://doi.org/10.1007/s11105-011-0408-0