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Expression Analysis of Defense-Related Genes in Cotton (Gossypium hirsutum) after Fusarium oxysporum f. sp. vasinfectum Infection and Following Chemical Elicitation using a Salicylic Acid Analog and Methyl Jasmonate

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Abstract

Cotton (Gossypium hirsutum) wilt caused by Fusarium oxysporum f. sp. vasinfectum (Fov) is considered as a major threat for commercial cotton production worldwide. Relative expression ratios of two key pathogenesis-related (PR) genes (PR-3 and PR-10) and a detoxification gene (GST18) were compared between a fully susceptible (“LACTA”) and a partially field-resistant (“EMERALD”) cultivar after challenging with an Australian Fov isolate, as well as after pre-treatments with chemical inducers of defense such as BION® (a chemical analog of salicylic acid) and methyl-jasmonate (MeJA) prior to Fov inoculation. It was demonstrated that in both hypocotyls and roots of “EMERALD”, all PR genes were over-expressed after inoculation with Fov but not in the fully susceptible cultivar. Fov inoculation did not significantly affect GST18 expression in both cultivars. Exogenous application of each defense elicitor, prior to Fov inoculation, resulted in up-regulation of the three genes in root tissues of the fully susceptible cultivar. BION® application did not influence PR-3 expression in hypocotyls of both cultivars, whereas MeJA application resulted in induction of PR-3 in both cultivars. Furthermore, in hypocotyls of “LACTA”, over-expression of PR-10 was recorded after treatment with each chemical inducer. This pathogen exhibited different ability in eliciting oxidative burst in roots of the two cotton cultivars used in our analysis.

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Acknowledgements

This research was funded by the General Secretariat for Research and Technology (GSRT, program PENED 10087), Greece. We thank S. ANDRIOTIS S.A. for providing “EMERALD” seeds, Mr. W. O' Neill (Department of Primary Industries, Indooroopilly, Queensland, Australia) for providing the Australian Fov isolates and P.A L. Dadurian for editing the manuscript.

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  1. Institute of Agrobiotechnology (IN.A.)—Center for Research and Technology Hellas (CERTH), Thermi, GR-570 01, Thessaloniki, Greece

    Antonios G. Zambounis, Mairi S. Kalamaki, Eleni E. Tani & Athanasios S. Tsaftaris

  2. Department of Plant Pathology, Agricultural University of Athens, 75 Iera Odos, GR-118 55, Athens, Greece

    Epameinondas J. Paplomatas

  3. Department of Genetics and Plant Breeding, Aristotle University of Thessaloniki, GR-540 06, Thessaloniki, Greece

    Athanasios S. Tsaftaris

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  1. Antonios G. Zambounis
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Correspondence to Athanasios S. Tsaftaris.

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Zambounis, A.G., Kalamaki, M.S., Tani, E.E. et al. Expression Analysis of Defense-Related Genes in Cotton (Gossypium hirsutum) after Fusarium oxysporum f. sp. vasinfectum Infection and Following Chemical Elicitation using a Salicylic Acid Analog and Methyl Jasmonate. Plant Mol Biol Rep 30, 225–234 (2012). https://doi.org/10.1007/s11105-011-0335-0

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