Abstract
A semidwarf line of Indica rice, Xinguiai, was derived from the progeny of a cross between the double dwarf mutant Xinguiaishuangai and the wild-type variety Nanjing 6. The semidwarf phenotype was controlled by the semidwarf gene, sdg. The second sheath and shoot elongation responses of the dwarf mutant to exogenous gibberellin (GA3) showed that sdg was insensitive to gibberellin (GA), and its endogenous GAs content was higher than that in wild-type cultivars. The SDG gene was cloned by a map-based cloning method and sequencing analysis revealed that the coding region of sdg had a single nucleotide substitution resulting in a single amino acid change from alanine to threonine. A cleaved amplified polymorphic sequence marker was designed according to sequences from mutant and wild-type materials. This sequence marker could be used to distinguish wild types and mutants, and thus, could be used for molecular marker-assisted selection. The dwarf phenotype of the sdg mutant was restored to a normal phenotype by introducing the wild-type SDG gene. Rice transformation experiments and GUS staining demonstrated that the SDG gene was predominantly expressed in vegetative organs.
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Acknowledgments
We thank Bin Han (National Center for Gene Research, China) for providing the BAC clone OSJNBb0079M13 and Zhukuan Cheng (Institute of Genetics and Developmental Biology, Chinese Academy of Science) for providing the plant binary vector pCAM23A. This study was financially supported by the National Natural Science Foundation of PR China (31071068), the Key Program of the Bureau of Education, Jiangsu Province, PR China (10KJA210059), and the National Project of Transgenic Crops of the Ministry of Science and Technology of PR China (2009ZX08009-100B).
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Sui, JM., Guo, BT., Wang, JS. et al. A New GA-Insensitive Semidwarf Mutant of Rice (Oryza sativa L.) with a Missense Mutation in the SDG Gene. Plant Mol Biol Rep 30, 187–194 (2012). https://doi.org/10.1007/s11105-011-0321-6
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DOI: https://doi.org/10.1007/s11105-011-0321-6