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Isolation and Partial Characterization of an R2R3MYB Transcription Factor from the Bamboo Species Fargesia fungosa

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Abstract

Formation of the woody stems of bamboo requires the coordinated regulation of cellulose, xylan, and lignin biosynthesis. Lignin contributes 25–30% of harvested bamboo constituents, and it strongly influences the physical properties of bamboo. In this study, we report on cloning and partial characterization of an R2R3MYB transcription factor gene (FfMYB1) from the bamboo species Fargesia fungosa. FfMYB1 consists of a coding region of 813 bp, corresponding to a predicted peptide of 270 amino acids, and an upstream promoter sequence of 1.46 kb. The deduced peptide sequence of FfMYB1 has the highest percent amino acid identity to NtMYBGR1 of tobacco, as well as to both AtMYB20 and AtMYB43 of Arabidopsis. Both NtMYBGR1 and AtMYB20/43 are putative activators of the phenylpropanoid pathway for lignin production. Histochemical analysis of F. fungosa stems showed rapid production of lignin during stem maturation, and FfMYB1 transcript was detected in leaves and stems of 1-year old shoots. A phylogenetic study of R2R3MYB sequences available for the bamboo subfamily identified other potential lignin-related R2R3MYBs, in particular bphylf044c24 of the bamboo genera Phyllostachys.

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Acknowledgments

The study was supported by grants from 948 Project of the Ministry of Forestry of China (Grant 2008-4-30), the National Basic Research Program of China 973 Program (Grant 2010CB434807), the Science and Technology Planning Project of Yunnan Province (Grant 2009CD073), and the Middle Aged Academic and Technical Leader Project of Yunnan Province (Grant 2010CI016).

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Authors and Affiliations

  1. Southwest Forestry University, Bailongsi 300, Kunming, Yunnan, China

    Juan Wang, Jing Wang & Yuming Yang

  2. New Zealand Institute for Plant & Food Research Limited, Private Bag, 11600, Palmerston North, New Zealand

    Huaibi Zhang & Kevin M. Davies

Authors
  1. Juan Wang
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  2. Jing Wang
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  3. Huaibi Zhang
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  4. Yuming Yang
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  5. Kevin M. Davies
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Corresponding author

Correspondence to Kevin M. Davies.

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Supplementary Figure 1

Phylogenetic relationship of FfMYB1, NtMYBGR1, and 49 P. heterocycla R2R3MYB ESTs to the A. thaliana R2R3MYB transcription factors. The full-length deduced amino acid sequence of FfMYB1 and NtMYBGR1 and the available sequences for the P. heterocycla ESTs were entered into the IT3F web-based comparison tool (Bailey et al. 2008) and a phylogenetic tree was formed against the A. thaliana and O. sativa R2R3MYB sequences (PDF 458 kb)

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Wang, J., Wang, J., Zhang, H. et al. Isolation and Partial Characterization of an R2R3MYB Transcription Factor from the Bamboo Species Fargesia fungosa . Plant Mol Biol Rep 30, 131–138 (2012). https://doi.org/10.1007/s11105-011-0319-0

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