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Gene Expression in Wheat Induced by Inoculation with Puccinia striiformis West

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat (Triticum aestivum L.) worldwide. A suppression subtractive hybridization (SSH) cDNA library was constructed from Pst-infected seedling leaves of an immune resistant germplasm Shaanmai 139. A total of 84 expressed sequence tags with high quality were obtained and alignment of the sequences identified in 56 Unigenes. Blast EST analysis of these Pst-induced genes showed that they were mostly homologous to genes that are induced by cold or heat stress. BlastX searches identified 45 of the Unigenes as similar to those encoding proteins of known function (e value < 1.00E-5), and eight of unknown function. The genes of known function include those with potential biological roles in signal transmission, energy and metabolism, transcription regulation, phenylpropanoid pathway, and defense response. Semi-quantitative reverse transcription-polymerase chain reaction studies confirmed that some of the cDNAs genes were differentially expressed in Pst-infected plants, with 24 and 72 hpi as the two critical time points for the induction of these genes by inoculation with P. striiformis West race CY32 in wheat. Bimodal patterns were presented in Pst-induced genes, such as senescence-associated genes, omega-6 fatty acid desaturase, and acyl-CoA synthetase. This suggests complex patterns of defense-related gene expression in multi-resistance gene cultivars in response to stripe rust infection.

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Acknowledgments

This work was financially supported by the prophase project of the National Key Basic Research and Development Program (973 Project) of China (2006CB708208) and the authors are grateful to the Natural Science Foundation of China and NWAFU preliminary research foundation for the funding. We would also like to thank Dr. Ming-Bo Wang and Wei-Ning Song for their useful suggestions and critical reading of this manuscript.

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Authors and Affiliations

  1. College of Agronomy, Northwest A & F University, Key Lab of Agricultural Molecular Biology of Shaanxi Province, Yangling, 712100, China

    Hong Zhang, Yingang Hu, Changyou Wang & Wanquan Ji

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  1. Hong Zhang
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  2. Yingang Hu
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  3. Changyou Wang
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  4. Wanquan Ji
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Correspondence to Wanquan Ji.

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Zhang, H., Hu, Y., Wang, C. et al. Gene Expression in Wheat Induced by Inoculation with Puccinia striiformis West. Plant Mol Biol Rep 29, 458–465 (2011). https://doi.org/10.1007/s11105-010-0245-6

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  • DOI: https://doi.org/10.1007/s11105-010-0245-6

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