Abstract
Storage of seeds for extended periods causes a number of degradative changes related to the aging process such as decreased seedling vigor and reduced germination. In this study, molecular markers were used to study the aging process in seeds of two different plants species. Seeds of three differentially aged seed groups, including control (un-aged), naturally aged, and accelerated aging, from soybean (Glycine max) and safflower (Carthamus tinctorius) were evaluated for genetic variability using random amplification of polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. For both plant species, naturally aged and accelerated aged groups clustered together with RAPD markers, whereas control and naturally aged seeds showed similarity in both AFLP and SSR profiles. Based on these findings, it can be concluded that observed changes in DNA profiles of seeds from different aged groups did not contribute to accumulation of genetic variations of the same magnitude. Therefore, seed of similar viability must be selected for molecular marker analysis for plant variety protection, among other comparative studies.
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References
Abdalla FH, Roberts EH (1968) Effects of temperature, moisture, and oxygen on the induction of chromosome damage in seeds of barley, broad beans, and peas during storage. Ann Bot (Lond) 32:119–136
Bednarek PT, Chwdorzewska K, Puchalski J (1998) Preliminary molecular studies on genetic changes in rye seeds due to long-term storage and regeneration. In: Gass T, Podyma W, Puchalski J, Eberhart SA (eds) Challenges in rye germplasm conservation. Int Plant Genet Resources Institute, pp 54–61
Boubriak I, Naumenko V, Lyne L, Osborne DJ (1999) Loss of viability in rye embryos at different levels of hydration: Senescence with apoptotic nucleosome cleavage or death with random DNA fragmentation. In: Black M, Bradford KJ, Vazquez-Ramos J (eds) Seed biology: advances and applications. CABI, Cambridge, pp 205–214
Chwedorzewska KJ, Bednarek PT, Puchalski J (2002a) AFLP-profiling long term stored and regenerated rye gene bank samples. Cell Mol Biol Lett 7:457–463
Chwedorzewska KJ, Bednarek PT, Puchalski J (2002b) Studies on changes in specific rye genome regions due to seed aging and regeneration. Cell Mol Biol Lett 7:569–576
Coello P, Vazquez-Ramos JM (1996) Maize DNA polymerase 2 (an alpha-type enzyme) suffers major damage after seed deterioration. Seed Sci Res 6:1–7. doi:10.1017/S0960258500002932
Cooke RJ, Bredemeijer GMM, Ganal MW, Peeters R, Isaac P, Rendell S, Jackson J, Roder MS, Korzun V, Wendehake K, Areshchenkaova T, Dijcks M, Laborie D, Bertrand L, Vosman B (2003) Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci. Euphytica 132:331–341. doi:10.1023/A:1025046919570
Cregan PB, Jarvik T, Bush AL, Shoemaker RC, Lark KG, Kahler AL, Kaya N, Vantoai TT, Lohnes DG, Chung J, Specht JE (1999) An integrated genetic linkage map of the soybean genome. Crop Sci 39:1464–1490
Devaiah SP, Pan X, Hong Y, Roth M, Welti R, Wang X (2007) Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis. Plant J 50:950–957. doi:10.1111/j.1365-313X.2007.03103.x
Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breed 118:369–390. doi:10.1046/j.1439-0523.1999.00401.x
Gutierrez G, Cruz F, Moreno J, Gonzalez-Hernandez VA, Vazquez-Ramos JM (1993) Natural and artificial seed ageing in maize: germination and DNA synthesis. Seed Sci Res 3:279–285. doi:10.1017/S0960258500001896
ISTA (2004) International seed testing rules, 2004. International Seed Testing Association, Zurich, Switzerland
Kang HW, Cho YG, Yoon UH, Eun MY (1998) A rapid DNA extraction method for RFLP and PCR analysis from a single dry seed. Plant Mol Biol Rep 16:1–9. doi:10.1023/A:1007418606098
Larson RA (1997) In: Larson RA (ed) Naturally occurring antioxidants. Lewis Publications, Boca Raton
Liu F, Zhuang BC, Zhang JS, Chen SY (2000) Construction and analysis of soybean genetic map. Acta Genetica Sin 11:1018–1026. (Yichuan Xuebao)
Marcos-Filho J, McDonald MB (1998) RAPD profiles, germination and vigour of naturally and artificially aged soybean seeds. Seed Sci Tech 26:141–156
Marcos-Filho J, McDonald MB, Tekrony DM, Zhang J (1997) RAPD fragment profiles from deteriorating soybean seeds. Seed Technol 19:33–44
McDonald MB (1999) Seed deterioration: physiology, repair and assessment. Seed Sci Tech 27:177–237
Matsumura H, Liu B, Abe J, Takahashi R (2008) AFLP mapping of soybean maturity gene E4. J Hered 99:193–197. doi:10.1093/jhered/esm114
Murata M, Roos EE, Tsuchiya T (1981) Chromosome damage induced b artificial seed ageing in barley. I. Germinability and frequency of aberrant anaphases at first mitosis. Can J Genet Cytol 23:267–280
Panguluri SK, Janaiah K, Govil JN, Kumar PA, Sharma PC (2006) AFLP fingerprinting in Pigeonpea (Cajanus cajan (L.) Millsp.) and its wild relatives. Genet Resour Crop Evol 53:523–531. doi:10.1007/s10722-004-2031-5
Paniego N, Echaide M, Munoz M, Fernandez L, Torales S, Faccio P, Fuxan I, Carrera M, Zandomeni R, Suarez EY, Hopp HE (2002) Microsatellite isolation and characterization in sunflower (Helianthus annuus L.). Genome 45:34–43. doi:10.1139/g01-120
Paran I, Aftergoot E, Shifriss C (1998) Variation in Capsicum annum revealed by RAPD and AFLP markers. Euphytica 99:167–173. doi:10.1023/A:1018301215945
Rao LS, Usha Rani P, Deshmukh PS, Kumar PA, Panguluri SK (2007) RAPD and ISSR fingerprinting in cultivated chickpea (Cicer arietinum L.) and its wild progenitor Cicer reticulatum Ladizinsky. Genet Resour Crop Evol 54:1235–1244. doi:10.1007/s10722-006-9104-6
Roberts EH (1973) Loss of seed viability: chromosomal and genetic aspects. Seed Sci Tech 1:515–527
Roldá T. n-Arjona, R.R. Ariza. (2008) Repair and tolerance of oxidative DNA damage in plants. Mutat Res. doi:10.1016/j.mrrev.2008.07.003
Roos EE (1982) Induced genetic changes in seed germplasm during storage. In: Khan AA (ed) The physiology and biochemistry of seed development, dormancy and germination. Elsevier, New York, pp 409–434
Roos EE (1988) Genetic changes in a collection over time. Hortic Sci 23:86–90
Schoen DJ, David JL, Bataillon TM (1998) Deleterious mutation accumulation and the regeneration of genetic resources. Proc Natl Acad Sci U S A 95:394–399. doi:10.1073/pnas.95.1.394
Sehgal D, Raina SN (2005) Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica 146:67–76. doi:10.1007/s10681-005-8496-2
Sehgal D, Rajpal VR, Raina SN, Sasanuma T, Sasakuma T (2008) Assaying polymorphism at DNA level for genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) world germplasm resources. Genetica. doi:10.1007/s10709-008-9292-4
Shatters RG Jr, Schweder ME, West SH, Abdelghany A, Smith RL (1995) Environmentally induced polymorphisms detected by RAPD analysis of soybean seed DNA. Seed Sci Res 5:109–116. doi:10.1017/S0960258500002683
Song QJ, Quigley CV, Nelson RL, Carter TE, Boerma HR, Strachan JL, Cregan PB (1999) A selected set of trinucleotide simple sequence repeat markers for soybean cultivar identification. Plant Var Seeds 12:207–220
Vijay D, Dadlani M (2003) Seed longevity and water absorption patterns in maize, soybean and safflower. Ind J Plant Physiol 8(Special issue):244–248
Villiers TA (1973) Aging and longevity of seeds. In: Heydecker W (ed) Seed ecology. Pennsylvania State University Press, University Park, USA, pp 265–288
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535. doi:10.1093/nar/18.22.6531
Wilson DO Jr, McDonald MB Jr (1986) The lipid peroxidation model of seed ageing. Seed Sci Tech 14:269–300
Wu XL, He CY, Wang YJ, Zhang ZY, Dongfang Y, Zhang JS, Chen SY, Gai JY (2001) Construction and analysis of a genetic linkage map of soybean. Acta Genetica Sin 11:1051–1061. (Yichuan Xuebao)
Xia Z, Tsubokura Y, Hoshi M, Hanawa M, Yano C, Okamura K, Ahmed TA, Anai T, Watanabe S, Hayashi M, Kawai T, Hossain KG, Masaki H, Asai K, Yamanaka N, Kubo N, Kadowaki K, Nagamura Y, Yano M, Sasaki T, Harada K (2007) An integrated high-density linkage map of soybean with RFLP, SSR, STS, and AFLP markers using A single F2 population. DNA Res 14:257–269. doi:10.1093/dnares/dsm027
Zhang J, McDonald MB, Sweeney PM (1996) Random amplified polymorphic DNA (RAPD) from seeds of differing soybean and maize genotypes. Seed Sci Tech 4:513–522
Zhang L, Huang BB, Kai GY, Guo ML (2006) Analysis of intraspecific variation of Chinese Carthamus tinctorius L. using AFLP markers. Yao Xue Xue Bao 41:91–96
Acknowledgments
The authors are thankful to Dr. B. M. Prasanna, Principal Scientist at Maize Genetics and Biotechnology in the Division of Genetics at the Indian Agricultural Research Institute for providing the radioactive material and equipment for the AFLP analysis. We also thank Andrew Marsh of the James Graham Brown Cancer Center at the University of Louisville for editing.
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Vijay, D., Dadlani, M., Kumar, P.A. et al. Molecular Marker Analysis of Differentially Aged Seeds of Soybean and Safflower. Plant Mol Biol Rep 27, 282–291 (2009). https://doi.org/10.1007/s11105-008-0085-9
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DOI: https://doi.org/10.1007/s11105-008-0085-9