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Molecular Cloning and Characterization of an Ortholog of NPR1 Gene from Dongguan Dajiao (Musa spp. ABB)

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Abstract

The nonexpresser of pathogenesis-related gene 1 (NPR1) plays a pivotal role in systemic acquired resistance in plants. In this study, a novel full-length NPR1-like gene, designated MdNPR1 (accession number FJ357442), was isolated by RACE-PCR from cv. Dongguan Dajiao (Musa spp. ABB), a local banana cultivar known to be resistant to Fusarium oxysporum fsp. cubense (FOC) race 4. Sequence alignment showed that MdNPR1 contained an ankyrin repeat domain and a broad complex, tramtrack, and bric-a-brac (BTB) domain. Semiquantitative reverse transcription polymerase chain reaction revealed that MdNPR1 could be constitutively expressed at low levels in both of the FOC race 4-susceptible cultivar Fenjiao (Musa spp. ABB) and the resistant cultivar Dongguan Dajiao. However, MdNPR1 could be induced by exogenous application of salicylic acid in cv. Dongguan Dajiao, but not in cv. Fenjiao. Moreover, the accumulated level of MdNPR1 transcripts in cv. Dongguan Dajiao was higher than that in cv. Fenjiao when plants were treated with FOC race 4 inoculation. Our results implied that MdNPR1 might represent as a promising candidate for engineering resistant to broad-spectrum pathogen in banana.

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Abbreviations

BTB/POZ:

broad complex, tramtrack, and bric-a-brac/pox virus and zinc finger

FOC:

Fusarium oxysporum fsp. Cubense

NPR:

nonexpresser of pathogenesis-related gene

PR:

pathogenesis-related

ORF:

open reading frame

RACE:

rapid amplification of cDNA ends

SA:

salicylic acid

SAR:

systemic acquired resistance

TGA:

subclass of bZIP transcription factors that bind to cognate as-1-type (TGACGT) cis-promoter elements

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (30400287), Guangdong Natural Science Foundation of China (04300538, 06023159, and 06300470), Guangdong Scientific and Technical Foundation (2006B20101014), and Guangzhou Scientific and Technical Foundation (2006Z3-E0281).

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Authors and Affiliations

  1. The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou, 510275, China

    Jie-Tang Zhao, Xia Huang, Ya-Ping Chen, Yun-Feng Chen & Xue-Lin Huang

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  1. Jie-Tang Zhao
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  2. Xia Huang
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  3. Ya-Ping Chen
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  4. Yun-Feng Chen
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Correspondence to Xue-Lin Huang.

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Zhao, JT., Huang, X., Chen, YP. et al. Molecular Cloning and Characterization of an Ortholog of NPR1 Gene from Dongguan Dajiao (Musa spp. ABB). Plant Mol Biol Rep 27, 243–249 (2009). https://doi.org/10.1007/s11105-008-0074-z

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