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Isolation of Six Novel Aquaporin Genes from Triticum aestivum L. and Functional Analysis of TaAQP6 in Water Redistribution

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Abstract

Water transport through the cell membranes is regulated largely by a family of proteins known as aquaporins (AQPs). In this study, six novel aquaporin genes, TaAQP1∼TaAQP6, were isolated from the common wheat Triticum aestivum L. var. Changle 5. These six TaAQPs share common features with other plant AQPs. Three of them (TaAQP2, 3, and 4) phylogenetically belong to plant PIP2 group, and the other three resemble proteins from PIP1(TaAQP1), TIP1(TaAQP6), and TIP2(TaAQP5) groups, respectively. The subcellular localization assay, determined by onion epidermal cell transient expression system, indicated that TaAQP6 is a membrane-associated protein. Transformation of TaAQP6 into Xenopus oocytes demonstrated that it facilitated water uptake, suggesting that this TaAQP is an active membrane-associated protein. The expression of TaAQP6 was upregulated in shoots of wheat seedlings, but downregulated in roots under polyethylene glycol (PEG) stress. In split-root experiment, the amount of TaAQP6 transcripts decreased in dry roots (cultured in PEG solution), but increased in wet roots (cultured in water), and remained unchanged in shoots. It is interesting to note that the osmolality changes of different parts in PEG-treated and split-root seedlings were related to the expression of TaAQP6. Taken together, we proposed that TaAQP6 participates in internal water redistribution of wheat seedlings during osmotic stress by regulating outflow of water from water-rich organs to the parts with poor water status under water deficit condition.

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Abbreviations

AQP:

aquaporin

MIP:

major intrinsic protein

Pf:

permeability coefficient

PEG:

polyethylene glycol

ABA:

abscisic acid

EST:

expressed sequence tag

RACE:

rapid amplification cDNA end

GFP:

green fluorescent protein

BS:

Barth’s solution

Reference

  • Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, Galili G. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress. Plant Cell 2003;15:439–47.

    Article  PubMed  CAS  Google Scholar 

  • Alexandersson E, Fraysse L, Sjövall-Larsen S, Gustavsson S, Fellert M, Karlsson M, Johanson U, Kjellbom P. Whole gene family expression and drought stress regulation of aquaporins. Plant Mol Biol. 2005;59:469–84.

    Article  PubMed  CAS  Google Scholar 

  • Aroca R, Amodeo G, Fernández-Illescas S, Herman EM, Chaumont F, Chrispeels MJ. The role of aquaporins and membrane damage in chilling and hydrogen peroxide induced changes in the hydraulic conductance of maize roots. Plant Physiol. 2005;137:341–53.

    Article  PubMed  CAS  Google Scholar 

  • Barrowclough DE, Peterson CA, Steudle E. Radial hydraulic conductivity along developing onion roots. J Exp Bot. 2000;51:547–57.

    Article  PubMed  CAS  Google Scholar 

  • Boursiac Y, Chen S, Luu D-T, Sorieul M, van den Dries N, Maurel C. Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant Physiol. 2005;139:790–805.

    Article  PubMed  CAS  Google Scholar 

  • Chaumont F, Barrieu F, Wojcik E, Chrispeels MJ, Jung R. Aquaporins constitute a large and highly divergent protein family in maize. Plant Physiol. 2001;125:1206–15.

    Article  PubMed  CAS  Google Scholar 

  • Claros MG, von Heijne G. TopPred II: an improved software for membrane protein structure predictions. Comput Appl Biosci. 1994;10:685–86.

    PubMed  CAS  Google Scholar 

  • Daniels MJ, Chaumont F, Mirkov TE, Chrispeel MJ. Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site. The Plant Cell 1996;8:587–99.

    Article  PubMed  CAS  Google Scholar 

  • Gustavsson S, Lebrun AS, Nordén K, Chaumont F, Johanson U. A novel plant major intrinsic protein in Physcomitrella patens most similar to bacterial glycerol channels. Plant Physiol. 2005;139:287–95.

    Article  PubMed  CAS  Google Scholar 

  • Jahn TP, Møller ALB, Zeuthen T, Holmb LM, Klæke DA, Mohsin B, KÜhlbrandt W, Schjoerring JK. Aquaporin homologues in plants and mammals transport ammonia. FEBS Lett. 2004;574:31–6.

    Article  PubMed  CAS  Google Scholar 

  • Jang JY, Kim DG, Kim YO, Kim SJ, Kang H. An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana. Plant Mol Biol. 2004;54:713–25.

    Article  PubMed  CAS  Google Scholar 

  • Johanson U, Gustavsson S. A new subfamily of major intrinsic proteins in plants. Mol Biol Evol. 2002;19:456–61.

    PubMed  CAS  Google Scholar 

  • Johansson I, Karlsson M, Johanson U, Larsson C, Kjellbom P. The role of aquaporins in cellular and whole plant water balance. Biochim Biophys Acta 2000;1465:324–42.

    Article  PubMed  CAS  Google Scholar 

  • Johanson U, Karlsson M, Johansson I, Gustavsson S, SjÖvall S, Fraysse L, Weig AR, Kjellbom P. The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol. 2001;126:1358–69.

    Article  PubMed  CAS  Google Scholar 

  • Kaldenhoff R, Grote K, Zhu JJ, Zimmermann U. Significance of plasmalemma aquaporins for water-transport in Arabidopsis thaliana. Plant J. 1998;14:121–28.

    Article  PubMed  CAS  Google Scholar 

  • Kirch H-H, Vera-Estrella R, Golldack D, Quigley F, Michalowski CB, Barkla BJ, Bohnert HJ. Expression of water channel proteins in Mesembryanthemum crystallinum. Plant Physiol. 2000;123:111–24.

    Article  PubMed  CAS  Google Scholar 

  • Lian HL, Yu X, Lane D, Sun WN, Tang ZC, Su WA. Upland rice and lowland rice exhibited different PIP expression under water deficit and ABA treatment. Cell Res. 2006;16:651–60.

    Article  PubMed  CAS  Google Scholar 

  • Lian HL, Yu X, Ye Q, Ding XS, Kitagawa Y, Kwak S, Su WA, Tang ZC. The role of aquaporin RWC3 in drought avoidance in rice. Plant Cell Physiol. 2004;45:481–89.

    Article  PubMed  CAS  Google Scholar 

  • Maurel C, Reizer J, Schroeder JI, Chrispeels MJ. The vacuolar membrane protein γ-TIP creates water specific channels in Xenopus oocytes. EMBO J. 1993;12:2241–47.

    PubMed  CAS  Google Scholar 

  • Maurel C. Aquaporins and water permeability of plant membranes. Annu Rev Plant Physiol Plant Mol Biol. 1997;48:399–429.

    Article  PubMed  CAS  Google Scholar 

  • Maurel C. Plant aquaporins: novel functions and regulation properties. FEBS Lett. 2007;581:2227–36.

    Article  PubMed  CAS  Google Scholar 

  • Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M. Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol. 2005;46:1568–77.

    Article  PubMed  CAS  Google Scholar 

  • Sakuratani T, Aoe T, Higuchi H. Reverse flow in roots of Sesbania rostrata measured using the constant power heat balance method. Plant Cell Environ. 1999;22:1153–60.

    Article  Google Scholar 

  • Sambrook J, Russell DW, editors. Molecular cloning: a laboratory manual, 3rd ed. New York: Cold Spring Harbor Laboratory Press; 2001.

  • Suga S, Komatsu S, Maeshima M. Aquaporin isoforms responsive to salt and water stresses and phytohormones in radish seedlings. Plant Cell Physiol. 2002;43:1229–37.

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;24:4876–82.

    Article  Google Scholar 

  • Tyerman SD, Bohnert HJ, Maurel C, Steudle E, Smith JAC. Plant aquaproins: their molecular biology, biophysics and significance for plant water relations. J Exp Bot. 1999;50:1055–71.

    Article  CAS  Google Scholar 

  • Verslues PE, Bray EA. LWR1 and LWR2 are required for osmoregulation and osmotic adjustment in Arabidopsis. Plant Physiol. 2004;136:2831–42.

    Article  PubMed  CAS  Google Scholar 

  • Weig A, Deswarte C, Chrispeels MJ. The major intrinsic protein family of Arabidopsis has 23 members that form three distinct groups with functional aquaporins in each group. Plant Physiol. 1997;114:1347–57.

    Article  PubMed  CAS  Google Scholar 

  • Yamada S, Katsuhara M, Kelly WB, Michalowski CB, Bohnert HJ. A family of transcripts encoding water channel proteins: tissue-specific expression in the common ice plant. Plant Cell 1995;7:1129–42.

    Article  PubMed  CAS  Google Scholar 

  • Zhou HB, Li SF, Deng ZY, Wang X, Chen T, Zhang JS, Chen SY, Ling HQ, Zhang AM, Wang DW, Zhang XQ. Molecular analysis of three new receptor-like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection. Plant J. 2007;52:420–34.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants from Ministry of Science and Technology of the People's Republic of China (No.2006BAD13B02) and the Chinese Academy of Sciences (No. KSCX2-YW-N-046).

We are grateful to Prof. Daowen Wang (State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for helpful suggestions and discussions on this manuscript. We thank Prof. Jian Zhang, Dr. Wei Zhang, and Dr. Huawei Wang (Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for their theoretical and practical help with heterologous expression in Xenopus oocytes.

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, People’s Republic of China

    Junfeng Zhang, Zhiyong Deng, Shuanghe Cao, Xianping Wang, Aimin Zhang & Xiangqi Zhang

  2. Graduate University of Chinese Academy of Sciences, Yuquan Road, Shijingshan District, Beijing, 100093, China

    Junfeng Zhang

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  1. Junfeng Zhang
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  2. Zhiyong Deng
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Correspondence to Xiangqi Zhang.

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Zhang, J., Deng, Z., Cao, S. et al. Isolation of Six Novel Aquaporin Genes from Triticum aestivum L. and Functional Analysis of TaAQP6 in Water Redistribution. Plant Mol Biol Rep 26, 32–45 (2008). https://doi.org/10.1007/s11105-008-0020-0

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