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Paraburkholderia sp. GD17 improves tomato plant growth and resistance to Botrytis cinerea-induced disease

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Abstract

Background and aims

The plant growth promoting rhizobacteria have been repeatedly addressed in improving plant growth and resistance against pathogens. This study explored the role of Paraburkholderia sp. GD17 in improving tomato plant growth and resistance to Botrytis cinerea (Bc).

Methods

Tomato roots were treated with GD17 strain, and then the leaves were inoculated with Bc. Physiological and biochemical parameters, and gene expression were analyzed.

Results

In the absence of Bc, GD17 efficiently improved plant growth, and increased photosynthetic efficiency. In the presence of Bc, GD17-bacterized plants exhibited an enhanced resistance, as indicated by 67% of disease index in non-bacterized plants, while by 24% in bacterized ones. In response to Bc, the defense reaction was reinforced in bacterized plants, as shown by enhanced antioxidative capacity and mitigated oxidative damage, as well as increased PR gene expression in bacterized plants compared with control. Photosynthesis was inhibited by Bc inoculation, to a greater degree in non-bacterized plants than in bacterized ones. In the presence of Bc, soluble sugar contents significantly increased in non-bacterized plants, while it was controlled in bacterized plants. The carbohydrate catabolism-related genes, including starch degradation, photorespiration, and pentose phosphate pathway, generally presented a higher expression in bacterized plants under Bc attack.

Conclusions

GD17 strain improved tomato plant growth by increasing the photosynthetic efficiency. GD17 enhanced plant resistance against Bc-induced disease by increasing defense and alleviating oxidative damage. Additionally, GD17 optimized the trade-off between plant growth and defense by strengthening carbohydrate metabolic regulation.

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Data availability

The data that support the fndings of this study are available from the corresponding authors upon request.

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Acknowledgements

The authors thank Prof. Howe (Michigan State University) for kindly providing the tomato seeds used in this study. This research was supported by the National Natural Science Foundation of China (Grant No. 31572213 to HL).

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  1. College of Life Science, Shenyang Normal University, No. 253, Huanghe North Street, Huanggu District, Shenyang City, 110034, China

    Anqi Gu, Danni Zhao, Huaqiang Liu, Wei Fu, Guangzhe Li & Lin Hao

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  1. Anqi Gu
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  2. Danni Zhao
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  3. Huaqiang Liu
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  4. Wei Fu
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  5. Guangzhe Li
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  6. Lin Hao
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Contributions

HL and LG designed and carried out the research. GA, ZD, LH and FW contributed to carry out the physiological, biochemical and the qRT-PCR analyses. HL and FW analyzed the data and wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Lin Hao.

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Gu, A., Zhao, D., Liu, H. et al. Paraburkholderia sp. GD17 improves tomato plant growth and resistance to Botrytis cinerea-induced disease. Plant Soil 486, 487–502 (2023). https://doi.org/10.1007/s11104-023-05890-2

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