Abstract
Purpose
Green manure plays a key role in reducing chemical fertilizer applications and increasing soil organic carbon (SOC) stock. The effects of chemical fertilizer substitution by Chinese milk vetch (MV) on the distribution and composition of organic carbon fractions in macroaggregates and microaggregates and SOC stability mechanism were investigated in paddy soils in southern China.
Methods
A 10-year (2008–2018) field experiment was conducted, including no fertilizer (CK), 100% NPK fertilizer (F100), MV with different percentages of chemical fertilizer (MV + F100, MV + F80, MV + F60 and MV + F40). The soil was separated into distinct organic carbon fractions using aggregate density fractionation and SOC chemical structure was analyzed by fourier-transform infrared and nuclear magnetic resonance.
Results
Chemical fertilizer substitution by MV increased SOC contents in the bulk soil by 9.1% (MV + F80), 5.8% (MV + F60), 17.9% (MV + F40) compared to F100. Organic carbon fraction mainly existed in mineral associated organic carbon (mSOC), accounting for 70.3–83.7% and 69.4–84.0% of the relative mass of macroaggregates and microaggregates, respectively. Compared to F100, aromatic C increased by 11.6% and 29.1% under MV + F60 within mSOC in macroaggregates and microaggregates. Within the mSOC in macroaggregates, compared to CK, MV + F80 and MV + F60 promoted the proportion of alkyl C by 9.6% and 6.7%, and decreased the content of O-alkyl C by 14.1% and 11.0%, respectively, which correspondingly increased alkyl C/O-alkyl C ratio.
Conclusions
Substitution of chemical fertilizer by MV (especially MV + F80 and MV + F60) improved the stability of SOC via increasing recalcitrant structure in mSOC, and was conducive to the sequestration of SOC in paddy soils.
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References
Artz RRE, Chapman SJ, Jean Robertson AH et al (2008) FTIR spectroscopy can be used as a screening tool for organic matter quality in regenerating cutover peatlands. Soil Biol Biochem 40:515–527. https://doi.org/10.1016/j.soilbio.2007.09.019
Balesdent J, Chenu C, Balabane M (2000) Relationship of soil organic matter dynamics to physical protection and tillage. Soil Tillage Res 53:215–230. https://doi.org/10.1016/S0167-1987(99)00107-5
Benbi DK, Toor AS, Kumar S (2012) Management of organic amendments in rice-wheat cropping system determines the pool where carbon is sequestered. Plant Soil 360:145–162. https://doi.org/10.1007/s11104-012-1226-3
Chai Y, Zeng X, E S, et al (2019) The stability mechanism for organic carbon of aggregate fractions in the irrigated desert soil based on the long-term fertilizer experiment of China. Catena 173:312–320. https://doi.org/10.1016/j.catena.2018.10.026
Chen M, Zhang S, Liu L et al (2022) Organic fertilization increased soil organic carbon stability and sequestration by improving aggregate stability and iron oxide transformation in saline-alkaline soil. Plant Soil. https://doi.org/10.1007/s11104-022-05326-3
Chen X, Xu Y, Gao H et al (2018) Biochemical stabilization of soil organic matter in straw-amended, anaerobic and aerobic soils. Sci Total Environ 625:1065–1073. https://doi.org/10.1016/j.scitotenv.2017.12.293
Chen Y, Liu X, Hou Y et al (2021) Particulate organic carbon is more vulnerable to nitrogen addition than mineral-associated organic carbon in soil of an alpine meadow. Plant Soil 458:93–103. https://doi.org/10.1007/s11104-019-04279-4
Clemente JS, Gregorich EG, Simpson AJ et al (2012) Comparison of nuclear magnetic resonance methods for the analysis of organic matter composition from soil density and particle fractions. Environ Chem 9:97. https://doi.org/10.1071/EN11096
Duan Y, Chen L, Zhang J et al (2021) Long-term fertilisation reveals close associations between soil organic carbon composition and microbial traits at aggregate scales. Agric Ecosyst Environ 306:107169. https://doi.org/10.1016/j.agee.2020.107169
Elliott ET (1986) Aggregate structure and carbon, nitrogen, and phosphorus in native and cultivated soils. Soil Sci Soc Am J 50:627–633. https://doi.org/10.2136/sssaj1986.03615995005000030017x
Fulton-Smith S, Cotrufo MF (2019) Pathways of soil organic matter formation from above and belowground inputs in a Sorghum bicolor bioenergy crop. GCB Bioenergy 11:971–987. https://doi.org/10.1111/gcbb.12598
Gao S, Cao W, Zhou G, Rees RM (2021) Bacterial communities in paddy soils changed by milk vetch as green manure: A study conducted across six provinces in South China. Pedosphere 31:521–530. https://doi.org/10.1016/S1002-0160(21)60002-4
Garcia-Franco N, Albaladejo J, Almagro M, Martínez-Mena M (2015) Beneficial effects of reduced tillage and green manure on soil aggregation and stabilization of organic carbon in a Mediterranean agroecosystem. Soil Tillage Res 153:66–75. https://doi.org/10.1016/j.still.2015.05.010
Helfrich M, Ludwig B, Buurman P, Flessa H (2006) Effect of land use on the composition of soil organic matter in density and aggregate fractions as revealed by solid-state 13C NMR spectroscopy. Geoderma 136:331–341. https://doi.org/10.1016/j.geoderma.2006.03.048
Jiang M, Wang X, Liusui Y et al (2017) Variation of soil aggregation and intra-aggregate carbon by long-term fertilization with aggregate formation in a grey desert soil. Catena 149:437–445. https://doi.org/10.1016/j.catena.2016.10.021
Kamran M, Huang L, Nie J et al (2021) Effect of reduced mineral fertilization (NPK) combined with green manure on aggregate stability and soil organic carbon fractions in a fluvo-aquic paddy soil. Soil Tillage Res 211:105005. https://doi.org/10.1016/j.still.2021.105005
Khan MI, Gwon HS, Alam MA et al (2020) Short term effects of different green manure amendments on the composition of main microbial groups and microbial activity of a submerged rice cropping system. Appl Soil Ecol 147:103400. https://doi.org/10.1016/j.apsoil.2019.103400
Kopittke PM, Dalal RC, Hoeschen C et al (2020) Soil organic matter is stabilized by organo-mineral associations through two key processes: The role of the carbon to nitrogen ratio. Geoderma 357:113974. https://doi.org/10.1016/j.geoderma.2019.113974
Lan X, Shan J, Huang Y et al (2022) Effects of long-term manure substitution regimes on soil organic carbon composition in a red paddy soil of southern China. Soil Tillage Res 221:105395. https://doi.org/10.1016/j.still.2022.105395
Lavallee JM, Soong JL, Cotrufo MF (2020) Conceptualizing soil organic matter into particulate and mineral-associated forms to address global change in the 21st century. Glob Change Biol 26:261–273. https://doi.org/10.1111/gcb.14859
Lehmann J, Kleber M (2015) The contentious nature of soil organic matter. Nature 528:60–68. https://doi.org/10.1038/nature16069
Li S, Gu X, Zhuang J et al (2016) Distribution and storage of crop residue carbon in aggregates and its contribution to organic carbon of soil with low fertility. Soil Tillage Res 155:199–206. https://doi.org/10.1016/j.still.2015.08.009
Liang C-H, Yin Y, Chen Q (2014) Dynamics of soil organic carbon fractions and aggregates in vegetable cropping systems. Pedosphere 24:605–612. https://doi.org/10.1016/S1002-0160(14)60046-1
Liu Y, Liu W, Wu L et al (2018) Soil aggregate-associated organic carbon dynamics subjected to different types of land use: Evidence from 13C natural abundance. Ecol Eng 122:295–302. https://doi.org/10.1016/j.ecoleng.2018.08.018
Lu R (2000) Analytical methods of soil agrochemistry. Chinese Agriculture Science and Technology Press, Beijing
Okolo CC, Gebresamuel G, Zenebe A et al (2020) Accumulation of organic carbon in various soil aggregate sizes under different land use systems in a semi-arid environment. Agric Ecosyst Environ 297:106924. https://doi.org/10.1016/j.agee.2020.106924
Pedersen JA, Simpson MA, Bockheim JG, Kumar K (2011) Characterization of soil organic carbon in drained thaw-lake basins of Arctic Alaska using NMR and FTIR photoacoustic spectroscopy. Org Geochem 42:947–954. https://doi.org/10.1016/j.orggeochem.2011.04.003
Prietzel J, Müller S, Kögel-Knabner I et al (2018) Comparison of soil organic carbon speciation using C NEXAFS and CPMAS 13C NMR spectroscopy. Sci Total Environ 628–629:906–918. https://doi.org/10.1016/j.scitotenv.2018.02.121
Pu Y, Lang S, Wang A et al (2022) Distribution and functional groups of soil aggregate-associated organic carbon along a marsh degradation gradient on the Zoige Plateau. China Catena 209:105811. https://doi.org/10.1016/j.catena.2021.105811
Rodriguez AF, Gerber S, Inglett PW et al (2021) Soil carbon characterization in a subtropical drained peatland. Geoderma 382:114758. https://doi.org/10.1016/j.geoderma.2020.114758
Rumpel C, Eusterhues K, Kögel-Knabner I (2010) Non-cellulosic neutral sugar contribution to mineral associated organic matter in top- and subsoil horizons of two acid forest soils. Soil Biol Biochem 42:379–382. https://doi.org/10.1016/j.soilbio.2009.11.004
Six J, Bossuyt H, Degryze S, Denef K (2004) A history of research on the link between (micro)aggregates, soil biota, and soil organic matter dynamics. Soil Tillage Res 79:7–31. https://doi.org/10.1016/j.still.2004.03.008
Six J, Elliott E, t., Paustian K, Doran JW, (1998) Aggregation and soil organic matter accumulation in cultivated and native grassland soils. Soil Sci Soc Am J 62:1367–1377. https://doi.org/10.2136/sssaj1998.03615995006200050032x
Six J, Elliott ET, Paustian K (2000) Soil macroaggregate turnover and microaggregate formation: a mechanism for C sequestration under no-tillage agriculture. Soil Biol Biochem 32:2099–2103. https://doi.org/10.1016/S0038-0717(00)00179-6
Song HJ, Lee JH, Canatoy RC et al (2021) Strong mitigation of greenhouse gas emission impact via aerobic short pre-digestion of green manure amended soils during rice cropping. Sci Total Environ 761:143193. https://doi.org/10.1016/j.scitotenv.2020.143193
Song W, Shu A, Liu J et al (2022) Effects of long-term fertilization with different substitution ratios of organic fertilizer on paddy soil. Pedosphere 32:637–648. https://doi.org/10.1016/S1002-0160(21)60047-4
Spaccini R, Piccolo A (2009) Molecular characteristics of humic acids extracted from compost at increasing maturity stages. Soil Biol Biochem 41:1164–1172. https://doi.org/10.1016/j.soilbio.2009.02.026
Torres-Sallan G, Schulte RPO, Lanigan GJ et al (2017) Clay illuviation provides a long-term sink for C sequestration in subsoils. Sci Rep 7:45635. https://doi.org/10.1038/srep45635
Totsche KU, Amelung W, Gerzabek MH et al (2018) Microaggregates in soils. J Plant Nutr Soil Sci 181:104–136. https://doi.org/10.1002/jpln.201600451
Voisin A-S, Guéguen J, Huyghe C et al (2014) Legumes for feed, food, biomaterials and bioenergy in Europe: a review. Agron Sustain Dev 34:361–380. https://doi.org/10.1007/s13593-013-0189-y
Wagai R, Kishimoto-Mo AW, Yonemura S et al (2013) Linking temperature sensitivity of soil organic matter decomposition to its molecular structure, accessibility, and microbial physiology. Glob Change Biol 19:1114–1125. https://doi.org/10.1111/gcb.12112
Walkley A, Black IA (1934) An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38. https://doi.org/10.1097/00010694-193401000-00003
Wang Q, Wen Y, Zhao B et al (2021) Coastal soil texture controls soil organic carbon distribution and storage of mangroves in China. Catena 207:105709. https://doi.org/10.1016/j.catena.2021.105709
Wen Y, Tang Y, Wen J et al (2021) Variation of intra-aggregate organic carbon affects aggregate formation and stability during organic manure fertilization in a fluvo-aquic soil. Soil Use Manag 37:151–163. https://doi.org/10.1111/sum.12676
Witzgall K, Vidal A, Schubert DI et al (2021) Particulate organic matter as a functional soil component for persistent soil organic carbon. Nat Commun 12:4115. https://doi.org/10.1038/s41467-021-24192-8
Xu Q, Rui W, Bian X, Zhang W (2007) Regional differences and characteristics of soil organic carbon density between dry land and paddy field in China. Agric Sci China 6:981–987. https://doi.org/10.1016/S1671-2927(07)60137-0
Xue B, Huang L, Huang Y et al (2019) Effects of organic carbon and iron oxides on soil aggregate stability under different tillage systems in a rice–rape cropping system. Catena 177:1–12. https://doi.org/10.1016/j.catena.2019.01.035
Xue B, Huang L, Huang Y et al (2020) Straw management influences the stabilization of organic carbon by Fe (oxyhydr)oxides in soil aggregates. Geoderma 358:113987. https://doi.org/10.1016/j.geoderma.2019.113987
Yang Z, Zheng S, Nie J et al (2014) Effects of long-term winter planted green manure on distribution and storage of organic carbon and nitrogen in water-stable aggregates of reddish paddy soil under a double-rice cropping system. J Integr Agric 13:1772–1781. https://doi.org/10.1016/S2095-3119(13)60565-1
Yao Z, Zhang D, Liu N et al (2019) Dynamics and sequestration potential of soil organic carbon and total nitrogen stocks of leguminous green manure-based cropping systems on the loess plateau of China. Soil Tillage Res 191:108–116. https://doi.org/10.1016/j.still.2019.03.022
Yeasmin S, Singh B, Smernik RJ, Johnston CT (2020) Effect of land use on organic matter composition in density fractions of contrasting soils: A comparative study using 13C NMR and DRIFT spectroscopy. Sci Total Environ 726:138395. https://doi.org/10.1016/j.scitotenv.2020.138395
Zhang H, Wang D, Su B et al (2021) Distribution and determinants of organic carbon and available nutrients in tropical paddy soils revealed by high–resolution sampling. Agric Ecosyst Environ 320:107580. https://doi.org/10.1016/j.agee.2021.107580
Zhao Z, Gao S, Lu C et al (2021) Effects of different tillage and fertilization management practices on soil organic carbon and aggregates under the rice–wheat rotation system. Soil Tillage Res 212:105071. https://doi.org/10.1016/j.still.2021.105071
Zhou G, Chang D, Gao S et al (2021) Co-incorporating leguminous green manure and rice straw drives the synergistic release of carbon and nitrogen, increases hydrolase activities, and changes the composition of main microbial groups. Biol Fertil Soils 57:547–561. https://doi.org/10.1007/s00374-021-01547-3
Zhou G, Gao S, Lu Y et al (2020) Co-incorporation of green manure and rice straw improves rice production, soil chemical, biochemical and microbiological properties in a typical paddy field in southern China. Soil Tillage Res 197:104499. https://doi.org/10.1016/j.still.2019.104499
Zhou X, Lu Y, Liao Y et al (2019) Substitution of chemical fertilizer by Chinese milk vetch improves the sustainability of yield and accumulation of soil organic carbon in a double-rice cropping system. J Integr Agric 18:2381–2392. https://doi.org/10.1016/S2095-3119(18)62096-9
Acknowledgements
We would like to acknowledge all the staff of the long-term experiment at Nan County, Hunan Province of China. This research was supported by National Natural Science Foundation of People’s Republic of China (Grant No. 41977020) and China Agriculture Research System of MOF and MARA (CARS-22). Dr. Yuedong Liu helped in measuring NMR datas.
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Huang, Y., Huang, L., Nie, J. et al. Effects of substitution of chemical fertilizer by Chinese milk vetch on distribution and composition of aggregates-associated organic carbon fractions in paddy soils. Plant Soil 481, 641–659 (2022). https://doi.org/10.1007/s11104-022-05668-y
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DOI: https://doi.org/10.1007/s11104-022-05668-y