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Exploring the yeast-mycorrhiza-plant interaction: Saccharomyces eubayanus negative effects on arbuscular mycorrhizal formation in tomato plants

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Abstract

Aims

Many studies have reported beneficial effects of yeasts on the colonization and development of arbuscular mycorrhizae, thought a few studies have also shown neutral effects. All these studies have in common that the mechanism, by which yeasts and mycorrhizae interact, is little understood. Here, we explore how plant growth-promoting yeasts affect the colonization of tomato plants by beneficial mycorrhizal fungi.

Methods

We tested the influence of the soil yeasts Candida saitoana, Tausonia pullulans, and Saccharomyces eubayanus on colonization of tomato roots by the mycorrhizal fungus Rhizophagus irregularis. We analyzed mycorrhizal parameters and the expression pattern of mycorrhiza-specific genes. In plants co-inoculated with S. eubayanus and R. irregularis, we measured the root accumulation pattern of jasmonic acid, oxo-phytodienoic acid, abscisic acid and salicylic acid, and the expression of genes related to plant hormone signaling and metabolism.

Results

The three yeasts had distinct effects on mycorrhizal colonization: C. saitoana had no effect on mycorrhizal parameters, T. pullulans delayed mycorrhizal colonization at an early stage, and S. eubayanus slowed colonization down throughout the entire trial. In plants co-inoculated with S. eubayanus and R. irregularis, we observed a sustained increase in jasmonic acid and up-regulation of the JA biosynthesis related genes LOXD, OPR3, and AOS1.

Conclusion

Co-inoculation with yeast affected mycorrhizal colonization and altered the expression pattern of mycorrhizal and plant defense-related genes. In particular, the yeast S. eubayanus modified plant defense hormones such as jasmonic acid, which is linked to mycorrhizal-induced resistance in tomato plants.

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Acknowledgements

LCMS analyses were carried out by Dr. Lourdes Sánchez-Moreno at the Scientific Instrumentation Service of the Estación Experimental del Zaidín (CSIC), Granada, Spain. This work was supported by Fondo para la Investigación Científica y Tecnológica (FONCYT) projects PICT2018-3441, Argentina. M.C.M. work at Estación Experimental del Zaidín (EEZ) was supported by a Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) partial fellowship for Assistant Researchers.

Funding

This work was supported by Fondo para la Investigación Científica y Tecnológica (FONCYT) projects PICT2018-3441, Argentina. M.C.M. work at Estación Experimental del Zaidín (EEZ) was supported by a Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) partial fellowship for Assistant Researchers.

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Authors and Affiliations

  1. Laboratorio de Microbiología Aplicada Y Biotecnología, IPATEC, Universidad Nacional del Comahue-CONICET, Quintral 1250, 8400, Bariloche, Río Negro, Argentina

    M. Cecilia Mestre

  2. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidı́n (EEZ), CSIC, Calle Profesor Albareda n# 1, 18008, Granada, Spain

    M. Isabel Tamayo Navarrete & J. Manuel García Garrido

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  1. M. Cecilia Mestre
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  2. M. Isabel Tamayo Navarrete
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  3. J. Manuel García Garrido
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Contributions

MCMestre and JMGarcía-Garrido contributed to the study conception and design. Material preparation and data collection was performed by MCMestre and MI Tamayo Navarrete; data analysis was performed by MCMestre and JM García-Garrido. The first draft of the manuscript was written by MCMestre and all authors commented on previous version of the manuscript. All author read and approved the final manuscript.

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Correspondence to M. Cecilia Mestre.

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Mestre, M.C., Tamayo Navarrete, M.I. & García Garrido, J.M. Exploring the yeast-mycorrhiza-plant interaction: Saccharomyces eubayanus negative effects on arbuscular mycorrhizal formation in tomato plants. Plant Soil 479, 529–542 (2022). https://doi.org/10.1007/s11104-022-05538-7

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