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OsGLO4 is involved in the formation of iron plaques on surface of rice roots grown under alternative wetting and drying condition

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Abstract

Background and aim

The molecular physiological mechanism of alternative wetting and drying (AWD) promoting iron plaque (IP) formation is unknown. Here, we report that a glycolate oxidase (GLO) gene, OsGLO4 contributes to AWD-induced IP formation.

Methods

Rice plants were grown in sand to explore the relationship among IP content, GLO activity, H2O2 concentration and OsGLO4 expression under AWD treatment.

Results

AWD significantly promoted GLO activity, OsGLO4 expression,and IP content in rice roots. Overexpressing OsGLO4 (OX) showed more 46.2% IP content than wild type (WT). Results with X-ray diffraction analysis indicated that OX also had a higher proportion of FeIII compound in IP in comparison to WT. The activity of GLO and H2O2 concentration in OX roots were 170.3% and 126.7% higher than those of WT. Interestingly, H2O2 distribution in epidermal cells was consistent with the spatial distribution of IP. Treatment with glycolic acid raised GLO activity by 88.9% and IP content by 33.3%; while α-hydroxy-2-pyridinemethanesulfonic acid inhibited GLO activity and reduced IP content. Exogenous H2O2 or treatment with aminotriazole increased the quantities of IP significantly, while dimethylthiourea reduced it.

Conclusion

OsGLO4 is involved in the formation of IP by mediating GLO activity and H2O2 production under AWD condition.

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Abbreviations

AWD:

alternative wetting and drying

IP:

iron plaque

CF:

continuous flooding

NRIP:

non-reddish brown iron plaque

RIP:

reddish brown iron plaque

GLO:

glycolate oxidase

CAT:

catalase

H2O2 :

hydrogen peroxide

HPMS:

α-hydroxy-2-pyridinemethanesulfonic acid

GLC:

glycolic acid

AT:

3-amino-1,2,4-triazole

DMTU:

dimethylthiourea

DAB:

diaminobenzidine

DO:

dissolved oxygen

ROL:

radial oxygen loss

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Acknowledgements

We thank Professor Xinxiang Peng (South China Agricultural University) for assisting with OsGLO4 overexpression lines seeds. This work was supported by National Natural Science Foundation of China (grant no. 31372125).

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Authors and Affiliations

  1. College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Xiao-Li Yu, Xu-Jian Yang & Hong Shen

  2. College of Forestry & Landscape Architecture, South China Agricultural University, Guangzhou, 510642, People’s Republic of China

    Dao-Ming Wu

  3. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, People’s Republic of China

    You-Qiang Fu

  4. Department of Plant Cell Biology, IZMB, Univerisity of Bonn, D-53115, Bonn, Germany

    František Baluška

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  1. Xiao-Li Yu
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  2. Dao-Ming Wu
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  5. František Baluška
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Correspondence to Hong Shen.

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Responsible Editor: Michael A. Grusak.

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Yu, XL., Wu, DM., Fu, YQ. et al. OsGLO4 is involved in the formation of iron plaques on surface of rice roots grown under alternative wetting and drying condition. Plant Soil 423, 111–123 (2018). https://doi.org/10.1007/s11104-017-3493-5

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