Abstract
In rice, low phosphorus results in the formation of reddish-brown iron plaque on the root surface, affecting the absorption of different elements by roots. In this paper, three different types of rice varieties were used as materials, and the transcriptomic and proteomic analysis, real-time fluorescence quantitative PCR (qRT-PCR), and OsLPR1 gene knockout techniques were employed to study the regulation of the OsLPR1 gene on the formation of iron plaque on root under low phosphorus. The transcriptomics and qRT-PCR analysis results showed that the transcription of five OsLPR1 homologous genes in roots of the three tested rice varieties was significantly induced by low-phosphorus treatment. Proteomic analysis results demonstrated that low-phosphorus treatment increased LPR1 protein content in rice roots compared with the control. Furthermore, the formation of iron plaque on rice root surface under low-phosphorus treatment was significantly inhibited by OsLPR1 gene knockout. These results further evidence that low phosphorus-induced OsLPR1 gene expression promotes the formation of iron plaque on rice root surface.
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The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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This research was supported by the Special Fund for Agro-Scientific Research in the Public Interest (No. 201103007), the National Natural Science Foundation Item (No. 31671618), and the Priority Academic Program Development of Jiangsu Higher Education Institutions, China.
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All authors contributed to the study conception and design. GCL conceived the study, edited the manuscript, and supervised the work. WZG participated in conceiving the project and carried out most transcriptome and protein spectrometry data analysis. WYL participated in conceiving the project, provided financial support for the study, and supervised the work. DY and RML carried out most experimentation, contributed to the design of the study, and drafted the manuscript. Mo SR and LJ involved in material preparation. All authors read and approved the final manuscript.
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Ding, Y., Ren, M., Mo, S. et al. Regulation of OsLPR1 gene on the formation of rice root iron plaque under low phosphorus. Plant Biotechnol Rep 16, 299–307 (2022). https://doi.org/10.1007/s11816-021-00737-7
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DOI: https://doi.org/10.1007/s11816-021-00737-7