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Modulatory effects of Mesorhizobium tianshanense and Glomus intraradices on plant proline and polyamine levels during early plant response of Lotus tenuis to salinity

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Abstract

Aims

The study aims (1) to evaluate the effect of Mesorhizobium tianshanense on plant proline and polyamine levels of Lotus tenuis and its modulatory effect during plant response to short-term salt stress and (2) to compare these effects with those caused by mycorrhizal symbiosis.

Methods

Experiments consisted of a randomized factorial design of two factors: salinity (two levels, 0 and 150 mM NaCl) and symbiosis (three levels, uninoculated, Glomus intraradices, and M. tianshanense).

Results

Salinization led to increased proline levels regardless of plant organ and symbiotic status, excepting mycorrhizal L. tenuis roots. Salinity diminished the total polyamine level of control and rhizobial plants but not in mycorrhizal ones. Variations in the pattern response of the three individual polyamines (putrescine, spermidine, and spermine) differed in accordance with the symbiotic status of the plant, highlighting a divergence on proline and polyamine metabolisms between rhizobial and mycorrhizal symbiosis.

Conclusions

Spermidine and spermine contributed the most with the salt-induced root polyamine increment observed upon salinization in roots of nodulated plants, suggesting that these polyamines might mediate an adaptive role of the plant–M. tianshanense symbiosis in L. tenuis plants growing in a saline environment.

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Abbreviations

DAO:

Diamine oxidase

PA:

Polyamines

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

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Acknowledgments

This work was supported by: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina), Agencia Nacional de Promoción Científica y Tecnológica (PICT 20517), UBACYT x143. M.E is CONICET scholarship holder. A.B.M. is a Universidad de Buenos Aires (UBA) and CONICET researcher, and O.A.R. and A.I.S are CONICET researchers.

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Authors and Affiliations

  1. CONICET-FAUBA, Facultad de Agronomía, Universidad de Buenos Aires, INBA, Instituto de Investigaciones en Biociencias Agrícolas y Ambientales, Av. San Martín 4453, (C1417DSE), Buenos Aires, CABA, Argentina

    Mariela Echeverria

  2. Unidad de Biotecnología 3, IIB-IINTECH/UNSAM-CONICET, Buenos Aires, Argentina

    Analía Inés Sannazzaro, Oscar Adolfo Ruiz & Ana Bernardina Menéndez

  3. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

    Ana Bernardina Menéndez

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  1. Mariela Echeverria
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  2. Analía Inés Sannazzaro
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  3. Oscar Adolfo Ruiz
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  4. Ana Bernardina Menéndez
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Correspondence to Mariela Echeverria.

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Echeverria, M., Sannazzaro, A.I., Ruiz, O.A. et al. Modulatory effects of Mesorhizobium tianshanense and Glomus intraradices on plant proline and polyamine levels during early plant response of Lotus tenuis to salinity. Plant Soil 364, 69–79 (2013). https://doi.org/10.1007/s11104-012-1312-6

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