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Molybdenum improves antioxidant and osmotic-adjustment ability against salt stress in Chinese cabbage (Brassica campestris L. ssp. Pekinensis)

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Abstract

Aims

A pot experiment was conducted to determine the effects of molybdenum on antioxidative defense and osmotic-adjustment systems of Chinese cabbage under salt stress.

Methods

Molybdenum fertilizer was applied at three levels (0, 0.15, 0.3 mg kg−1). Ten days after sowing, 500 ml 136.8 mM of NaCl solution was added to half of the plants for each treatment every 10th day for three consecutive times.

Results

The results revealed that with the application of molybdenum in Chinese cabbage under salt stress the fresh weight significantly increased; activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were dramatically improved; the contents of non-enzymatic antioxidants such as glutathione (GSH), carotenoid (CAR) and ascorbic acid (ASA) were significantly increased. There was also an significant increase in low molecular osmotic-adjustment products such as soluble sugar, soluble protein and proline. Moreover, molybdenum significantly increased potassium ion (K+) content and reduced sodium ion (Na+) contents, which eventually improved the K+/Na+ ratios.

Conclusions

The present study suggests that the application of molybdenum enhances the salt stress tolerance in Chinese cabbage by increasing the capacity to eliminate active oxygen and the ability of osmotic-adjustment.

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Abbreviations

Mo:

Molybdenum

SOD:

Superoxide dismutase

POD:

Peroxidase

CAT:

Catalase

ASA:

Ascorbate

GSH:

Reduced glutathione

CAR:

Carotene

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Acknowledgements

The authors acknowledge Dr Di (Soil, Plant and Ecological Sciences Division, Lincoln University, Canterbury, New Zealand) for critical reviewing and revision of the manuscript. Financial support by the Fundamental Research Funds for the Central Universities(Program No.2010PY025, 2010PY150)and Innovation Funds of Huazhong Agricultural University (52902-0900206044) are greatly acknowledged.

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Authors and Affiliations

  1. Micro-element Research Center, Huazhong Agricultural University, Wuhan, 430070, China

    Mu Zhang, Chengxiao Hu, Xiaohu Zhao, Qiling Tan, Xuecheng Sun, Anyong Cao, Min Cui & Ying Zhang

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  1. Mu Zhang
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  2. Chengxiao Hu
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  3. Xiaohu Zhao
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  7. Min Cui
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Correspondence to Chengxiao Hu.

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Responsible Editor: Timothy J. Flowers.

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Zhang, M., Hu, C., Zhao, X. et al. Molybdenum improves antioxidant and osmotic-adjustment ability against salt stress in Chinese cabbage (Brassica campestris L. ssp. Pekinensis). Plant Soil 355, 375–383 (2012). https://doi.org/10.1007/s11104-011-1109-z

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