Abstract
To examine the effects of subsoil NaCl salinity in relation to water stress imposed at different growth stages, wheat was grown in a heavy texture clay soil (vertosol) under glasshouse conditions in polythene lined cylindrical PVC pots (100 cm long with 10.5 cm diameter) with very low salinity level (ECe 1.0 dS/m; ESP 1.0 and Cl 30 mg/kg soil) in top 10 cm soil (10–20 cm pot zone) and low salinity level (ECe 2.5 dS/m, ESP 5, and Cl 100 mg/kg soil) in top 10–20 cm soil (20–30 cm pot zone). The plants were exposed to three subsoil salinity levels in the 20–90 cm subsoil (30–100 cm pot zone) namely low salinity (ECe: 2.5 dS/m, ESP: 5, Cl: 100 mg/kg soil), medium salinity (ECe: 4.0 dS/m, ESP: 10, Cl: 400 mg/kg) and high salinity (ECe: 11.5 dS/m, ESP: 20, Cl: 1950 mg/kg) in the subsoil (20–90 cm soil layer: 30–100 cm pot zone). Watering of plants was withheld for 20 days commencing at either early booting or anthesis or mid grain filling, and then resumed until maturity, and these treatments were compared with no water stress. Water stress commencing at anthesis stage had the most depressing effect on grain yield and water use efficiency of wheat followed by water stress at grain filling stage and early booting stage. High subsoil salinity reduced grain yield by 39.1, 24.3%, and 13.4% respectively in plants water-stressed around anthesis, early booting, and mid grain filling compared with 36.6% in well-watered plants. There was a significant reduction in root biomass, rooting depth, water uptake and water use efficiency of wheat with increasing subsoil salinity irrespective of water regimes. Plants at high subsoil salinity had 64% of their root biomass in the top 0–30 cm soil and there was a marked reduction in subsoil water uptake. Roots also penetrated below the non-saline surface into salinised subsoil and led to attain high concentration of Na and Cl and reduced Ca/Na and K/Na ratio of flag leaf at anthesis stage. Results suggest that high subsoil salinity affects root growth and water uptake, grain yield and water use efficiency even in well water plants. Water stress at anthesis stage had the most depressing effect on wheat.
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References
Al-Karaki GN (2001) Germination, sodium and potassium concentration of barley seeds as influenced by salinity. J Plant Nutr 24:511–522
Ashraf M, Athar HR, Harris PJC, Kwon TR (2008) Some prospective strategies for improving crop salt tolerance. Adv Agron 97:45–110
Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Aust J Biol Sci 15:413–428
Botella MA, Martinez V, Pardines J, Cerda A (1997) Salinity induce potassium deficiency in maize plants. J Plant Physiol 150:200–205
Chhipa BR, Lal P (1995) Na/K ratios as the basis of salt tolerance in wheat. Aust J Agric Res 46:533–539
Dang YP, Dalal RC, Mayer DG, McDonald M, Routley R, Schwenke GD, Buck SR, Daniells IG, Singh DK, Manning W, Ferguson N (2008) High subsoil chloride concentrations reduce soil water extraction and crop yield on Vertosols in north-eastern Australia. Aust J Agric Res 59:321–330
Dang YP, Routley R, McDonald M, Dalal RC, Singh DK, Orange D, Mann M (2006) Subsoil constraints in Vertosols: crop water use, nutrient concentration and grain yield of bread wheat, durum wheat, barley, chickpea and canola. Aust J Agric Res 57:983–998
Francois LE, Maas EV, Donovan TJ, Youngs VL (1986) Effect of salinity on grain yield and quality, vegetative growth and germination of semi-dwarf and durum wheat. Agron J 78:1053–1058
Glenn E, Brown JJ, Blumwald E (1999) Salt-tolerate mechanisms and crop potential of halophytes. Crit Rev Plant Sci 18:227–255
Grattan SR, Grieve CM (1999) Salinity-mineral nutrient relations in horticultural crops. Hort Sci 78:127–157
Grieve CM, Francois LE, Mass EV (1994) Salinity affects the timing of phasic development in spring wheat. Crop Sci 34:1544–1549
Grewal HS, Norrish S, Cornish P (2004) Subsoil salts affects root function, shoot growth and ionic balance of wheat plants. New directions for a diverse planet: In Proceedings of the 4th International Crop Science Congress. 26 Sep–1 Oct 2004. Brisbane, Australia.
Kara SM, Keser S (2001) Effect of salinity on plant growth and mineral constituents of maize (Zea mays). Indian J Agric Sci 71:371–374
Khatun S, Flowers TJ (1995) Effect of salinity on seed set in rice. Plant Cell Environ 18:61–87
Katerji N, Mastrorilli M, van Horn JW, Lahmer FZ, Hamdy A, Oweis T (2009) Durum wheat and barley productivity in saline-drought environments. Eur J Agron 31:1–9
Mass EV, Grieve C (1990) Spike and leaf development in salt-stressed wheat. Crop Sci 30:1309–1313
Mass EV, Lesch SM, Francois LE, Grieve CM (1994) Tiller development in salt stressed wheat. Crop Sci 34:1594–1603
Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239–250
Munns R (2005) Genes and salt tolerance: bringing them together. New Phytol 167:645–663
Munns R, Gardner A, Tonnet ML, Rawson HM (1988) Growth and development in NaCl-treated plants. II. Do Na+ or Cl− concentration in dividing or expanding tissues determines growth in barley? Aust J Plant Physiol 15:529–540
Muranaka S, Shimizu K, Kato M (2002) Ionic and osmotic effects of salinity on single-leaf photosynthesis in two wheat cultivars with different drought tolerance. Photosynthetica 40:201–207
Naidu R, Rengasamy P (1993) Ion interactions and constraints to plant nutrition in Australia. Aust J Soil Res 31:801–819
Nuttall JG, Armstrong RD, Connor DJ (2003) Evaluating physiochemical constraints of Calcarosols on wheat yield in the Victorian southern Mallee. Aust J Agric Res 54:487–497
Rengasamy P (2002) Transient salinity and subsoil constraints to dryland farming in Australian sodic soils: an overview. Aust J Exp Agric 42:351–361
Rengasamy P (2006) World salinization with emphasis on Australia. J Exp Bot 57:1017–1023
Rengasamy P, Chittleborough D, Helyar K (2003) Root-zone constraints and plant-based solutions for dryland salinity. Plant Soil 257:249–260
Rengel Z (1992) The role of calcium in salt toxicity. Plant Cell Environ 15:625–632
Sairam RK, Srivastava GC (2002) Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Sci 162:897–904
Storey R, Gorham K, Pitman MC, Hanson MG, Gage D (1993) Response of Melanthera biflora to salinity and water stress. J Exp Bot 44:1551–1561
Subbarao GV, Johnson C, Jana MK, Rao JVDK (1990) Effects of sodium/calcium ratio in modifying salinity response of pigeonpea (Cajanus cajan). J Plant Physiol 136:439–443
Tarakcioglu C, Inal A (2002) Changes induced by salinity, demarcating specific ion ratio (Na/Cl) and osmolality in ion and proline accumulation, nitrate reductase activity, and growth performance of lettuce. J Plant Nutr 25:27–41
Wang D, Shannon MC, Grieve CM (2001) Salinity reduces radiation absorption and use efficiency in soybean. Field Crops Res 69:267–277
Wong MTF, Asseng S (2007) Yield and environmental benefits of ameliorating subsoil constraints under variable rainfall in a Mediterranean environment. Plant Soil 297:29–42
Yemm EW, Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57:508–514
Zarcinas BA, Carwright B, Spouncer LR (1987) Nitric acid digestion and multi-element analysis of plant material by inductively coupled plasma spectrometry. Commun Soil Sci Plant Anal 18:131–146
Acknowledgement
The author acknowledges the help of Dr Shane Norrish, Professor Peter Cornish and technical staff (Mr Burhan Amiji, Mr Mark Emanuel and Ms Linda Allanson) for their help during this study. The work was supported by the Grains Research and Development Corporation of Australia (SIP08 project).
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Singh Grewal, H. Response of wheat to subsoil salinity and temporary water stress at different stages of the reproductive phase. Plant Soil 330, 103–113 (2010). https://doi.org/10.1007/s11104-009-0179-7
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DOI: https://doi.org/10.1007/s11104-009-0179-7