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Effects of aqueous root extracts and hydrophobic root exudates of cucumber (Cucumis sativus L.) on nuclei DNA content and expression of cell cycle-related genes in cucumber radicles

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Abstract

Allelopathic interactions implicate the inhibition of cell division by allelochemicals. To examine the effects of autotoxic agents on cell cycle and plant growth, germinated cucumber seeds (Cucumis sativus L.) were incubated in solutions containing the aqueous root extracts of cucumber at 1:100, 1:50, 1:25 and 1:10 (w:v), or the hydrophobic root exudates of cucumber at 25, 50 and 100 mg·L−1. Aqueous root extracts and hydrophobic root exudates inhibited radicle elongation by 36.47–60.18% and 38.24–62.50%, respectively. The mitosis-specific genes were down-regulated in roots exposed to aqueous root extracts and hydrophobic root exudates. Meanwhile, exposure to either aqueous root extracts or hydrophobic root exudates decreased the proportion of 2C (C-value) and increased the proportion of 8C, leading to an increased mean C-value. We conclude that autotoxic agent-induced inhibition of radicle growth was partly attributed to the down-regulation of cell cycle-related genes and endoreduplication was enhanced under our experimental condition.

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Acknowledgement

This work was supported by the National Basic Research Program of China (2009CB11900), National Key Project of Scientific and Technical Supporting Programs of China (2008BADA6B02, 2006BAD07B03).

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Authors and Affiliations

  1. Department of Horticulture, Huajiachi Campus, Zhejiang University, Kaixuan Road 268, Hangzhou, 310029, P.R. China

    Yun Zhang, Min Gu, Kai Shi, Yan Hong Zhou & Jing Quan Yu

  2. Key Laboratory of Horticultural Plants Growth, Development and Biotechnology, Agricultural Ministry of China, Hangzhou, P.R. China

    Jing Quan Yu

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  1. Yun Zhang
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  2. Min Gu
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  4. Yan Hong Zhou
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Correspondence to Jing Quan Yu.

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Responsible Editor: Harsh P. Bais.

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Zhang, Y., Gu, M., Shi, K. et al. Effects of aqueous root extracts and hydrophobic root exudates of cucumber (Cucumis sativus L.) on nuclei DNA content and expression of cell cycle-related genes in cucumber radicles. Plant Soil 327, 455–463 (2010). https://doi.org/10.1007/s11104-009-0075-1

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