Abstract
In this study, the toxic effects of epiclorohidrine (ECh) were investigated in vivo by Allium test. The toxic effects have been investigated in terms of physiological, cytogenetic, anatomical, and biochemical aspects. The changes in germination percentage, weight gain, and root length were investigated as physiological parameter; micronucleus (MN), mitotic index (MI), and chromosomal abnormality (CA) frequencies were as cytogenetic parameter. Oxidative stress indicators such as superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were analyzed for biochemical changes and also damages in root tip cells were evaluated as anatomical parameter. It was determined that germination percentage, weight gain, root length, and MI decreased; MN and CA frequencies were increased with the increase of ECh treatment dose. ECh treatment caused significant increase in SOD and CAT enzyme activities and MDA levels and these results indicated a stress formation. A variety of anatomical changes and damages were observed in the root tip cells induced by ECh. In conclusion, the toxic effects of ECh on A. cepa which is a model of eucaryotic cell were investigated in a multi-directional way and serious toxic effects of ECh treatment were determined.
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References
Baker AJ (1981) Accumulators and excluders-strategies in the response of plants to heavy metals. J Plant Nutr 3:643–654
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140
Bridges BA (1978) On the detection of volatile liquid with bacteria: experiments with dichlorvos and epichlorohydrin. Mutat Res 54:367–371
Chen Y, Jungsuwadee P, Vore M, Butterfield DA, St Clair DK (2007) Collateral damage in cancer chemotherapy: oxidative stress in nontargeted tissues. Mol Interv 7:147–156
Crews C, Hough P, Brereton P, Harvey D, Macarthur R, Matthews W (2002) Survey of 3-monochloropropane-1,2-diol (3- MCPD) in selected food groups. Food Addit Contam 19:22–27
Daniel FB, Robinson M, Olson GR, Page NP (1996) Toxicity studies of epichlorohydrin in Sprague-Dawley rats. Drug Chem Toxicol 19:41–58
Darbelley N, Driss-Ecole D, Perbal G (1989) Elongation and mitotic activity of cortical cells in lentil roots grown in microgravity. Plant Physiol Biochem 27:341–347
Demirtas G, Cavusoglu K, Yalcin E (2018) Anatomic, physiologic and cytogenetic changes in Allium cepa L. induced by diniconazole. Cytologia 80:51–57
Driss-Ecole D, Schoevaert D, Noin M, Perbal G (1994) Densitometric analysis of nuclear DNA content in lentil roots grown in space. Biol Cell 81:59–64
Dumitriu S (2005) Polysaccharides: structural diversity and functional versatility, Second edn. CRC press, New York
Fenench M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) Human micronucleus project. HUMN Project: detailed description of the scoring criteria for the cytokinesis-blok micronucleus assay using isolated human lymphocyte cultures. Mutat Res 534:65–75
Freuder E, Leake CD (1941) The toxicity of epichlorohydrin. U Calif Publ Pharmacol 2:69–78
Kirsch-Volders M, Elhajouji A, Cundari E, Van Hummelen P (1997) The in vitro micronucleus test: a multi-endpoint assay to detect simultaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutat Res 392:19–30
Liu H, Liao B, Lu S (2004) Toxicity of surfactant, acid rain and Cd2+ combined pollution to the nucleus of Vicia faba root tip cells. Chin J Appl Ecol 15:493–496
Ocak N, Cavusoglu K, Yalcin E (2018) Investigation of toxic effects of chromium (K2Cr2O7) in Allium cepa L. Gaziosmanpasa. J Sci Res 7:146–158
Rossi AM, Migliore L, Lascialfari D, Sbrana I, Loprieno N, Tortoreto M, Bidoli F, Pantarotto C (1983) Genotoxicity, metabolism and blood kinetics of epichlorohydrin in mice. Mutat Res 118:213–226
Sharma CBSR, Panneerselvam N, Ma TH (1990) Genetic toxicology of pesticides in higher plant systems. Crit Rev Plant Sci 9:409–442
Silveira GL, Lima MGF, dos Reis GB, Palmieri MJ, Andrade-Vieira LF (2017) Toxic effects of environmental pollutants: comparative investigation using Allium cepa L. and Lactuca sativa L. Chemosphere 178:359–367
Singh US, Decker-Samuelian K, Solomon JJ (1996) Reaction of epichlorohydrin with 2-deoxynucleosides: characterization of adducts. Chem Biol Interact 99:109–128
Sobrero MC, Ronco A (2004) Ensayo de toxicidad aguda con semillas de lechuga (Lactuca sativa L.). In: Castillo G (ed) Ensayos Toxicológicos Y Métodos de Evaluación de Calidad de Aguas. IDRC/IMTA, Capitulo, pp 71–79
Unyayar S, Celik A, Cekic FO, Gozel A (2006) Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba. Mutagenesis 21:77–81
Vanparys P, Vermeiren F, Sysmans M, Temmerman R (1990) The micronucleus assay as a test for the detection of aneugenic activity. Mutat Res 244:95–103
Waidyanatha S, Gaudette NF, Hong Y, Fennell TR (2014) Formation of epichlorohydrin, a known rodent carcinogen, following oral administration of 1, 3-dichloro-2-propanol in rats. Chem Res Toxicol 27:1787–1795
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Yalçın, E., Uzun, A. & Çavuşoğlu, K. In vivo epiclorohidrine toxicity: cytogenetic, biochemical, physiological, and anatomical evidences. Environ Sci Pollut Res 26, 22400–22406 (2019). https://doi.org/10.1007/s11356-019-05518-y
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DOI: https://doi.org/10.1007/s11356-019-05518-y