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Effect of soil biota on growth and allocation by Eucalyptus microcarpa

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Abstract

We examined growth of Eucalyptus microcarpa seedlings in soil collected from four sites in southeastern Australia, in which retired pasture land has been revegetated with mixed plantings of Eucalyptus and Acacia species. Revegetation of farm land in southeastern Australia is an area of major investment. The focus of the study was to examine the influence of soil biota on seedling growth and its possible interaction with soil enrichment from a legume (Acacia) and decomposition rates. We used a soil freezing treatment (−80°C for 3 days) to retard the soil biota, with the expectation that invertebrates in particular would be killed. Soil freezing did not cause a nutrient pulse, but did reduce the level of ammonium in soil. Nitrate levels increased with time in pots, regardless of the soil treatment. Decomposition rates measured using cellulose substrate were significantly reduced by the freeze treatment, but only for approximately 90 days. Eucalyptus microcarpa seedlings grown in freeze-treated soil were approximately 40% smaller (total biomass), had marginally lower LAR (leaf area ratio), and significantly lower LMA (leaf mass per area). Low LMA indicates that leaves are either thinner in cross-section or less dense. We hypothesise that both the poor growth of seedlings and production of less robust leaves are consequences of reduced availability of soil nutrients due to the diminished soil biota after freeze treatment. Litter under Acacia was richer in nitrogen than litter under Eucalyptus but there was no difference in nitrogen content of soil, and consequently no soil source effects on plant growth or decomposition. We suggest that variation in the soil biota has the potential to greatly enhance or hinder the success of revegetation on retired agricultural land, but enrichment of soil by decomposition of nitrogen rich litter in these sites requires longer than the 8–15 years since they were revegetated.

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Acknowledgments

We thank Alison Jeavons, Lindsay Ezard and Geoff Winsell (Department of Primary Industry, Victoria) for help with site selection, and the landholders for allowing us access to sites. Kim Pullen provided assistance in field. We thank Celeste Linde (Australian National University), Elizabeth Lindsay and Mike Hodda (CSIRO Entomology) for methodological advice and information on various groups of soil organisms. Financial support for this research was provided by the CSIRO Water for a Healthy Country Flagship.

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  1. School of Botany and Zoology, Australian National University, Canberra, ACT, 0200, Australia

    Mark Bourne & Adrienne B. Nicotra

  2. CSIRO Entomology, GPO Box 1700, Canberra, ACT, 2601, Australia

    Mark Bourne, Matthew J. Colloff & Saul A. Cunningham

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  1. Mark Bourne
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Correspondence to Saul A. Cunningham.

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Bourne, M., Nicotra, A.B., Colloff, M.J. et al. Effect of soil biota on growth and allocation by Eucalyptus microcarpa . Plant Soil 305, 145–156 (2008). https://doi.org/10.1007/s11104-007-9533-9

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