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Effect of Scleroderma Spore Density and Age on Mycorrhiza Formation and Growth of Containerized Eucalyptus globulus and E. urophylla Seedlings

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Abstract

Greenhouse trials with containerized Eucalyptus globulus and E. urophylla seedlings were made to determine effective inoculum spore densities (Scleroderma cepa from temperate Australia, and S. citrinum from sub-tropical China) and spore storage conditions (fresh spores or spores stored for 5 years at room temperature or at 4 °C; S. albidum, S. areolatum and S. cepa from Western Australia). Inoculation with 106 or 108 spores/seedling increased eucalypt growth by up to 46% in height and 42% in dry weight compared to non-inoculated seedlings at 12 weeks after inoculation. Mycorrhizal formation was poor at 102 spores/seeding. Spores stored at 4 °C for 5 years were as effective in forming mycorrhizas as freshly collected spores when a standard density of 106 spores/seedling was applied. It is recommended that spore densities ≥104 be used for inoculation of containerised eucalypts and that spores be stored at 4 °C until use.

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Authors and Affiliations

  1. School of Biological Sciences and Biotechnology, Murdoch University, Perth, WA 6150, Australia

    Ying Long Chen, Bernard Dell & Nicholas Malajczuk

  2. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China

    Ying Long Chen

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  1. Ying Long Chen
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  2. Bernard Dell
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Correspondence to Ying Long Chen.

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Chen, Y.L., Dell, B. & Malajczuk, N. Effect of Scleroderma Spore Density and Age on Mycorrhiza Formation and Growth of Containerized Eucalyptus globulus and E. urophylla Seedlings. New Forest 31, 453–467 (2006). https://doi.org/10.1007/s11056-005-0880-1

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