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Does carbon partitioning in ectomycorrhizal pine seedlings under elevated CO2 vary with fungal species?

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Abstract

Enhanced soil respiration in response to elevated atmospheric CO2 has been demonstrated, and ectomycorrhizal (ECM) fungi are of particular interest since they partition host-derived photoassimilates belowground. Although a strong response of ECM fungi to elevated CO2 has been shown, little is still known about the functional diversity among species. We studied carbon (C) partitioning in mycorrhizal Scots pine seedlings in response to short-term CO2 enrichment, using seven ECM species with different ecological strategies. Mycorrhizal associations were synthesised and seedlings grown in large Petri dishes containing peat:vermiculite and nutrient solution for 10–15 weeks, after which half of the microcosms were exposed to elevated CO2 treatment (710 ppm) for 15 days and the other half were kept in ambient CO2 treatment. Partitioning of C was quantified by pulse labelling the seedlings with 14CO2 and examining the distribution of labelled assimilates in shoot, root and extraradical mycelial compartments by destructive harvest and liquid scintillation counting. Fungal biomass was determined with PLFA analysis. The respiratory loss of 14CO2 was on average greater in the elevated CO2 treatment for most species compared to the ambient CO2 treatment. More label was retrieved in the shoots in the ambient CO2 treatment compared to elevated CO2 (significant for P. involutus and P. fallax). Greater amounts of label were found in the extraradical mycelial compartment in all species (except P. involutus) in elevated CO2 compared to ambient CO2 (significant for L. bicolor, P. byssinum, P. fallax and R. roseolus). Fungal biomass production increased significantly with elevated CO2 for two species (H. velutipes and A. muscaria); three species (P. fallax, P. involutus and R. roseolus) showed a similar but non-significant trend, whereas L. bicolor and P. byssinum produced less biomass in elevated CO2 compared to ambient CO2. When 14C in the mycelial compartment and respiration was expressed per unit fungal PLFA the difference between CO2 treatments disappeared. We demonstrated that different ECM fungal isolates respond differently in C partitioning in response to CO2 enrichment. These results suggest that under certain growth conditions, when nutrients are not limiting, ECM fungi respond rapidly to increasing C-availability through changed biomass production and respiration.

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Acknowledgements

We thank Francis Martin, Ulla Ahonen-Jonnart, Björn Lindahl and Andy Taylor for fungal isolates, and Dave Johnson for valuable input into the isotope work. We also thank Allan Sim who kindly assisted with technical support, Colin Campbell for feedback on PLFA extractions, and Jeri Parrent and Roger Finlay for reading the manuscript. Funding was provided by Carl Trygger’s foundation and the UK National Environment Research Council. ICA receives funding from the Scottish Executive Environment and Rural Affairs Department.

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Authors and Affiliations

  1. Department of Plant and Soil Science, School of Biological Sciences, University of Aberdeen, Cruickshank Building, Aberdeen, AB24 3UU, Scotland

    Petra M. A. Fransson & Ian J. Alexander

  2. The Macaulay Institute, Craigiebuckler, Aberdeen, AB15 8QH, Scotland

    Petra M. A. Fransson & Ian C. Anderson

  3. Department of Forest Mycology & Pathology, P.O. Box 7026, 75007, Uppsala, Sweden

    Petra M. A. Fransson

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  1. Petra M. A. Fransson
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  2. Ian C. Anderson
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  3. Ian J. Alexander
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Correspondence to Petra M. A. Fransson.

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Fransson, P.M.A., Anderson, I.C. & Alexander, I.J. Does carbon partitioning in ectomycorrhizal pine seedlings under elevated CO2 vary with fungal species?. Plant Soil 291, 323–333 (2007). https://doi.org/10.1007/s11104-007-9203-y

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