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Soil enzyme activities and organic matter composition in a turfgrass chronosequence

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Abstract

Highly managed turfgrass systems accumulate considerable soil organic C, which supports a diverse and robust soil microbial community. Degradation of this soil organic C is mediated by a suite of soil enzymes. The relationship between these enzyme activities and the quality of soil organic C is central to understanding the dynamics of soil organic matter. We examined the activities of several soil enzymes involved in microbial C acquisition, including β-glucosidase, N-acetyl-β-glucosaminidase, cellulase, chitinase, and phenol oxidase, and characterized the chemical composition of soil organic matter using Fourier transform infrared spectroscopy (FTIR) in a turfgrass chronosequence (1–95 years old) and adjacent native pines. Non-metric multidimensional scaling analysis showed that the chemical composition of soil organic matter varied with turf age and land use (turf versus pines). Using the polysaccharide peak (1,060 cm−1) as a reference, both aliphatic (2,930 cm−1) and carboxylic (1,650 and 1,380 cm−1) compounds increased with turf age, indicating that soil organic matter became more recalcitrant. Soil enzyme activities per unit soil mass increased with turf age and were correlated to soil C content. Most soil enzyme activities in native pines were similar to those in young turf, but the cellulase activity was similar to or greater than the activity in old turfgrass systems. On a soil C basis, however, the activities of N-acetyl-β-glucosaminidase and cellulase decreased with turf age; this reduction was correlated to the relative changes in the chemical composition of soil organic matter. We observed that the chemical composition of soil organic matter was significantly correlated with the enzyme activity profile when expressed per unit microbial biomass C, but not per unit soil organic C. Our results suggest that chemical composition of soil organic matter changes with turf age and this change partially determines the relative abundance of C-degrading soil enzymes, likely through the influence on microbial community composition.

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Acknowledgments

We thank the Center for Turfgrass Research and Education, North Carolina, USA for financial support and appreciate the assistance of Pinehurst Resort and Forest Creek Golf Club. We also thank Ms. Kim Hutchison for helping the FTIR analysis.

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Authors and Affiliations

  1. Soil Science Department, North Carolina State University, Raleigh, NC, 27695, USA

    Wei Shi, Emily Dell & Kannan Iyyemperumal

  2. Crop Science Department, North Carolina State University, Raleigh, NC, 27695, USA

    Daniel Bowman

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  1. Wei Shi
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  2. Emily Dell
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  3. Daniel Bowman
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  4. Kannan Iyyemperumal
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Correspondence to Wei Shi.

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Shi, W., Dell, E., Bowman, D. et al. Soil enzyme activities and organic matter composition in a turfgrass chronosequence. Plant Soil 288, 285–296 (2006). https://doi.org/10.1007/s11104-006-9116-1

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  • DOI: https://doi.org/10.1007/s11104-006-9116-1

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