Abstract
Salt stress adversely affects plant growth and development. It is necessary to understand the underlying salt response mechanism to improve salt tolerance in plants. MYB transcription factors can regulate plant responses to salt stress. However, only a few studies have explored the role of MYB TFs in Sorghum bicolor (L.) Moench. So we decided to make a systematic analysis and research on the sorghum MYB family. A total of 210 MYB genes in sorghum were identified in this study. Furthermore, 210 MYB genes were distributed across ten chromosomes, named SbMYB1-SbMYB210. To study the phylogeny of the identified TFs, 210 MYB genes were divided into six subfamilies. We further demonstrated that SbMYB genes have evolved under strong purifying selection. SbMYBAS1 (SbMYB119) was chosen as the study object, which the expression decreased under salt stress conditions. Further study of the SbMYBAS1 showed that SbMYBAS1 is located in the nucleus. Under salt stress conditions, Arabidopsis plants overexpressed SbMYBAS1 showed significantly lower dry/fresh weight and chlorophyll content but significantly higher membrane permeability, MDA content, and Na+/K+ ratio than the wild-type Arabidopsis plants. Yeast two-hybrid screening result showed that SbMYBAS1 might interact with proteins encoded by SORBI_302G184600, SORBI_3009G247900 and SORBI_3004G59600. Results also showed that SbMYBAS1 could regulate the expression of AtGSTU17, AtGSTU16, AtP5CS2, AtUGT88A1, AtUGT85A2, AtOPR2 and AtPCR2 under salt stress conditions. This work laid a foundation for the study of the response mechanism of sorghum MYB gene family to salt stress.
Key message
This report shows detailed characterization of sorghum MYB gene family and demonstrates that SbMYBAS1 plays an important role in negative regulation of salt tolerance by reducing ROS scavenging ability and K+ content in Arabidopsis.
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Data availability
The sorghum MYB protein sequences were collected from Ensembl Plants database (https://www.plants.ensembl.org/). The Arabidopsis MYB protein sequences were downloaded from TAIR database (http://www.arabidopsis.org/). RNA-Seq data were downloaded from NCBI SRA database under the following accession number, SRR11147325, SRR11147326, SRR11147327, SRR11147328, SRR11147329, SRR11147330, SRR11147331, SRR11147332, SRR11147333, SRR11147334, SRR11147335 and SRR11147336.
Abbreviations
- MYB:
-
Myeloblastosis
- TF:
-
Transcription factor
- HMM:
-
Hidden Markov Model
- ML:
-
Maximum likelihood
- GSDS:
-
Gene structure display server
- MEME:
-
Multiple Em for Motif Elicitation
- CDS:
-
Coding sequence
- MW:
-
Molecular weight
- pI:
-
Isoelectric point
- MCSscanX:
-
Multiple collinearity scan toolkit
- FPKM:
-
Fragments Per Kilobase of exon model per Million mapped fragments
- gDNA:
-
Genomic DNA
- RNA:
-
Ribonucleic acid
- qRT-PCR:
-
Real-time quantitative reverse transcription PCR
- ABRE:
-
Abscisic acid responsive element
- MeJARE:
-
Methyl jasmonate responsive element
- DSRE:
-
Defense and stress responsive element
- AURE:
-
Auxin responsive element
- SARE:
-
Salicylic acid responsive element
- GIRE:
-
Gibberellin responsive element
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Acknowledgements
The Agricultural Fine Seed Project of Shandong Province (2021LZGC006), the National Natural Science Research Foundation of China (32272040), the Natural Science Research Foundation of Shandong Province (ZR2023QC192), the National Key R&D Program of China (2022YFD1201700) and the special fund for TaiShan Scholars (No. tsqn202211106).
Funding
National Natural Science Research Foundation of China, 32272040,Na Sui,National Key R& D Program of China, 2022YFD1201700, Na Sui, Agricultural Fine Seed Project of Shandong Province, 2021LZGC006, Na Sui, Special fund for TaiShan Scholars, No.tsqn202211106, Na Sui
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ML, ZC, YD, XD and NS initiated the manuscript. ML, ZC, YD, JL, JW, HZ, SL, XW performed the experiments. All authors read and approved the final manuscript.
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11103_2023_1386_MOESM2_ESM.pdf
Supplementary file2 (PDF 996 KB)—(A) Phylogenetic analysis of the MYB family in sorghum. Using the protein amino acid sequences of sorghum to construct a phylogenetic
11103_2023_1386_MOESM5_ESM.pdf
Supplementary file5 (PDF 456 KB)—Chromosoma distribution of sorghum SbMYB genes. The number of chromosomes were at the middle of each chromosome and the red lines connect genes that were tandem duplicated genes. The scale was in megabases (Mb).
11103_2023_1386_MOESM8_ESM.pdf
Supplementary file8 (PDF 2033 KB)—Expression patterns of SbMYB genes according to the transcriptome data. Data represent mean values from three biological replicates (± S.D.), *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
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Lu, M., Chen, Z., Dang, Y. et al. Identification of the MYB gene family in Sorghum bicolor and functional analysis of SbMYBAS1 in response to salt stress. Plant Mol Biol 113, 249–264 (2023). https://doi.org/10.1007/s11103-023-01386-w
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DOI: https://doi.org/10.1007/s11103-023-01386-w