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Antagonistic function of the Ve R-genes in tomato

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Abstract

Key message In Verticillium wilt, gene silencing indicates that tomato Ve2-gene expression can have a dramatic effect on many defense/stress protein levels while Ve1-gene induction modulates these effects in a negative fashion.

Abstract

In tomato, Verticillium resistance is dependent on the Ve R-gene locus, which encodes two leucine-rich repeat receptor-like proteins, Ve1 and Ve2. During fungal wilt, Ve1 protein is sharply induced while Ve2 appears expressed constitutively throughout disease development; the disease resistance function usually is attributed to the Ve1 receptor alone. To study Ve2 function, levels of Ve2 mRNA were suppressed using RNAi in both susceptible and resistant Craigella tomato near-isolines and protein changes were evaluated at both the mRNA and protein levels. The results indicate that Ve2-gene expression can have dramatic effects on many defense/stress protein levels while the presence of intact Ve1 protein minimizes these effects in a negative fashion. The data suggest an antagonistic relationship between the Ve proteins in which Ve1 modulates the induction of defense/stress proteins by Ve2.

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Acknowledgements

We thank Drs. X. Luo and K.V. Soman (UTMB Proteomics Center) for the peptide mass spectrometry and help with the proteomic analyses. This study was supported by NSERC Canada (R.N.N. and J.R.) and NIH, NHLBI (A.K.).

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Ross N. Nazar, Xin Xu & Jane Robb

  2. Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA

    Alexander Kurosky

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  1. Ross N. Nazar
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  2. Xin Xu
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  3. Alexander Kurosky
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  4. Jane Robb
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RNN and JR conceived the experiments and prepared the manuscript, XX conducted the experiments. RNN conducted the data analyses and AK was responsible for the peptide mass spectrometry.

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Correspondence to Ross N. Nazar.

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Nazar, R.N., Xu, X., Kurosky, A. et al. Antagonistic function of the Ve R-genes in tomato. Plant Mol Biol 98, 67–79 (2018). https://doi.org/10.1007/s11103-018-0764-3

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  • DOI: https://doi.org/10.1007/s11103-018-0764-3

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