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A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence

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Abstract

Key message The OsPLS3 locus was isolated by map-based cloning that encodes a DUF266-containing protein. OsPLS3 regulates the onset of leaf senescence in rice.

Abstract

Glycosyltransferases (GTs) are one of the most important enzyme groups required for the modification of plant secondary metabolites and play a crucial role in plant growth and development, however the biological functions of most GTs remain elusive. We reported here the identification and characterization of a novel Oryza sativa premature leaf senescence mutant (ospls3). Through map-based cloning strategy, we determined that 22-bp deletion in the OsPLS3 gene encoding a domain of unknown function 266 (DUF266)-containing protein, a member of GT14-like, underlies the premature leaf senescence phenotype in the ospls3 mutant. The OsPLS3 mRNA levels progressively declined with the age-dependent leaf senescence in wild-type rice, implying a negative role of OsPLS3 in regulating leaf senescence. Physiological analysis, and histochemical staining and transmission electron microscopy assays indicated that the ospls3 mutant accumulated higher levels of ethylene and reactive oxygen species than its wild type. Furthermore, the ospls3 mutant showed hypersensitivity to exogenous 1-aminocyclopropane-1-carboxylic acid, H2O2 and high level of cytokinins. Our results indicated that the DUF266-containing gene OsPLS3 plays an important role in the onset of leaf senescence, in part through cytokinin and ethylene signaling in rice.

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Acknowledgements

This study was supported by the National Key Research and Development Program of China (No. 2017YFD0300100), the National Natural Science Foundation of China (No. 31771688) and the National Key Transform Program (2016ZX08001-002). The authors thank two anonymous reviewers for valuable comments.

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  1. Kunyu Li and Yaodong Chen have contributed equally to this work.

Authors and Affiliations

  1. Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Kunyu Li, Yaodong Chen, Yanmin Luo, Chaoyue Zhao, Fangmin Cheng & Gang Pan

  2. Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People’s Republic of China

    Fudeng Huang

  3. Experimental Teaching Center, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Xun Xiang

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  1. Kunyu Li
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Contributions

KL, YC, YL, XX, FC and GP conceived and designed the research. KL, YC, YL, and FH conducted the experiments. KL, YL, FH and GP analyzed data. KL and PG wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Gang Pan.

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Li, K., Chen, Y., Luo, Y. et al. A 22-bp deletion in OsPLS3 gene encoding a DUF266-containing protein is implicated in rice leaf senescence. Plant Mol Biol 98, 19–32 (2018). https://doi.org/10.1007/s11103-018-0758-1

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