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Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides

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Abstract

Development of hybrid rice has greatly contributed to increased yields during the past three decades. Two bentazon-lethal mutants 8077S and Norin8m are being utilized in developing new hybrid rice systems. When the male sterile lines are developed in such a mutant background, the problem of F1 seed contamination by self-seeds from the sterile lines can be solved by spraying bentazon at seedling stage. We first determined the sensitivity of the mutant plants to bentazon. Both mutants showed symptoms to bentazon starting from 100 mg/l, which was about 60-fold, lower than the sensitivity threshold of their wild-type controls. In addition, both mutants were sensitive to sulfonylurea-type herbicides. The locus for the mutant phenotype is bel for 8077S and bsl for Norin8m. Tests showed that the two loci are allelic to each other. The two genes were cloned by map-based cloning. Interestingly, both mutant alleles had a single-base deletion, which was confirmed by PCR-RFLP. The two loci are renamed bel a (for bel) and bel b (for bsl). The wild-type Bel gene encodes a novel cytochrome P450 monooxgenase, named CYP81A6. Analysis of the mutant protein sequence also revealed the reason for bel a being slightly tolerant than bel b. Introduction of the wild-type Bel gene rescued the bentazon- and sulfonylurea-sensitive phenotype of bel a mutant. On the other hand, expression of antisense Bel in W6154S induced a mutant phenotype. Based on these results we conclude that the novel cytochrome P450 monooxygenase CYP81A6 encoded by Bel confers resistance to two different classes of herbicides.

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, Zhejiang, China

    Gang Pan, Xianyin Zhang, Jun Zhu & Jumin Tu

  2. National Key Laboratory of Crop Genetic Improvement, Wuhan, 430070, Hubei, China

    Kede Liu

  3. Institue of Crop Breeding and Cultivation, Hubei Academy of Agricultural Science, Wuhan, 430064, Hubei, China

    Jiwen Zhang & Xiaozhi Wu

Authors
  1. Gang Pan
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  2. Xianyin Zhang
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  3. Kede Liu
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  4. Jiwen Zhang
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  5. Xiaozhi Wu
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  6. Jun Zhu
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  7. Jumin Tu
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Correspondence to Jumin Tu.

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Gang Pan and Xianyin Zhang contributed equally to this work.

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Pan, G., Zhang, X., Liu, K. et al. Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides. Plant Mol Biol 61, 933–943 (2006). https://doi.org/10.1007/s11103-006-0058-z

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  • DOI: https://doi.org/10.1007/s11103-006-0058-z

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