Plant Molecular Biology

, Volume 96, Issue 4–5, pp 403–416 | Cite as

Physiological and RNA-seq analyses provide insights into the response mechanism of the Cf-10-mediated resistance to Cladosporium fulvum infection in tomato

  • Guan Liu
  • Junfang Liu
  • Chunli Zhang
  • Xiaoqing You
  • Tingting Zhao
  • Jingbin Jiang
  • Xiuling Chen
  • He Zhang
  • Huanhuan Yang
  • Dongye Zhang
  • Chong Du
  • Jingfu Li
  • Xiangyang Xu
Article

Abstract

Key message

Based on the physiological and RNA-seq analysis, some progress has been made in elucidating the Cf-10-mediated resistance responses to C. fulvum infection in tomato. GO and KEGG enrichment analysis revealed that the DEGs were significantly associated with defense-signaling pathways like oxidation-reduction processes, oxidoreductase activity and plant hormone signal transduction.

Abstract

Leaf mold, caused by the fungus Cladosporium fulvum, is one of the most common diseases affecting tomatoes worldwide. Cf series genes including Cf-2, Cf-4, Cf-5, Cf-9 and Cf-10 play very important roles in resisting tomato leaf mold. Understanding the molecular mechanism of Cf gene-mediated resistance is thus the key to facilitating genetic engineering of resistance to C. fulvum infection. Progress has been made in elucidating two Cf genes, Cf -19 and Cf -12, and how they mediate resistance responses to C. fulvum infection in tomato. However, the mechanism of the Cf-10- mediated resistance response is still unclear. In the present study, RNA-seq was used to analyze changes in the transcriptome at different stages of C. fulvum infection. A total of 2,242 differentially expressed genes (DEGs) responsive to C. fulvum between 0 and 16 days post infection (dpi) were identified, including 1,501 upregulated and 741 downregulated genes. The majority of DEGs were associated with defense-signaling pathways including oxidation–reduction processes, oxidoreductase activity and plant hormone signal transduction. Four DEGs associated with plant-pathogen interaction were uniquely activated in Cf-10 tomato and validated by qRT-PCR. In addition, physiological indicators including reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were measured at 0–21 dpi, and hormone expression [Jasmonic acid (JA) and salicylic acid (SA)] was estimated at 0 and 16 dpi to elucidate the mechanism of the Cf-10-mediated resistance response. C. fulvum infection induced the activities of POD, CAT and SOD, and decreased ROS levels. JA was determined to participate in the resistance response to C. fulvum during the initial infection period. The results of this study provide accountable evidence for the physiological and transcriptional regulation of the Cf-10-mediated resistance response to C. fulvum infection, facilitating further understanding of the molecular mechanism of Cf-10-mediated resistance to C. fulvum infection.

Keywords

Cf-10 tomato RNA-seq Antioxidant enzymes and peroxidase Hormones Resistance response Cladosporium fulvum 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China [NSFC; 31272171], China Agriculture Research System [CARS-25-A-15], and the Breeding of New Staple Vegetable Varieties of Heilongjiang Province [GA15B103]. We thank the Biomarker Limited Company for the data processing of this manuscript. We also thank the Medjaden Bioscience Limited for editing this manuscript.

Author contributions

GL, TZ, JL, XX and JJ conceived and designed the experiments. XC, HZ, HY, XY and DZ performed the RNA isolation. CD prepared the materials. JL and CZ processed the data.GL performed the qRT-PCR, data analysis and wrote the manuscript. All authors reviewed and approved the final manuscript.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Guan Liu
    • 1
  • Junfang Liu
    • 1
  • Chunli Zhang
    • 1
  • Xiaoqing You
    • 1
  • Tingting Zhao
    • 1
  • Jingbin Jiang
    • 1
  • Xiuling Chen
    • 1
  • He Zhang
    • 1
  • Huanhuan Yang
    • 1
  • Dongye Zhang
    • 1
  • Chong Du
    • 1
  • Jingfu Li
    • 1
  • Xiangyang Xu
    • 1
  1. 1.College of Horticulture and Landscape ArchitecutureNortheast Agricultural UniversityHarbinChina

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