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Brassica villosa, a system for studying non-glandular trichomes and genes in the Brassicas

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Abstract

Brassica villosa is a wild Brassica C genome species with very dense trichome coverage and strong resistance to many insect pests of Brassica oilseeds and vegetables. Transcriptome analysis of hairy B. villosa leaves indicated higher expression of several important trichome initiation genes compared with glabrous B. napus leaves and consistent with the Arabidopsis model of trichome development. However, transcripts of the TRY inhibitory gene in hairy B. villosa were surprisingly high relative to B. napus and relative transcript levels of SAD2, EGL3, and several XIX genes were low, suggesting potential ancillary or less important trichome-related roles for these genes in Brassica species compared with Arabidopsis. Several antioxidant, calcium, non-calcium metal and secondary metabolite genes also showed differential expression between these two species. These coincided with accumulation of two alkaloid-like compounds, high levels of calcium, and other metals in B. villosa trichomes that are correlated with the known tolerance of B. villosa to high salt and the calcium-rich natural habitat of this wild species. This first time report on the isolation of large amounts of pure B. villosa trichomes, on trichome content, and on relative gene expression differences in an exceptionally hairy Brassica species compared with a glabrous species opens doors for the scientific community to understand trichome gene function in the Brassicas and highlights the potential of B. villosa as a trichome research platform.

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Acknowledgments

The authors thank K. Narayanan for assistance in B. villosa trichome preparation and Z. Baines of Plant Gene Resources Canada (Saskatoon) for investigating the conditions which promoted flowering in B. villosa. Dr. Angelina Morales and Dr. Branimir Gjetvaj are gratefully acknowledged for collection and analysis of ESI–MS spectra and photographic assistance, respectively. N. Nayidu and A. Taheri were recipients of NSERC Visiting Fellowships to a Canadian Government Laboratory. This research was supported by Grants from the Canola Council of Canada, the Western Canadian Canola Grower organizations, and funding from Agriculture and Agri-Food Canada.

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Authors and Affiliations

  1. Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N0X2, Canada

    Naghabushana K. Nayidu, Ali Taheri, Dwayne Hegedus & Margaret Y. Gruber

  2. Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, SK, S7N 5E2, Canada

    Naghabushana K. Nayidu

  3. National Research Council, 110 Gymnasium Place, Saskatoon, SK, S7N 0W9, Canada

    Yifang Tan & Jacek Nowak

  4. College of Plant Sciences, Jilin University, Changchun, 130062, Jilin, China

    Xiang Li

  5. Department of Biological Sciences and Computing Science, University of Alberta, 11043 90 Ave NW, Edmonton, AB, T6G 1A6, Canada

    Trent C. Bjorndahl & David S. Wishart

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  1. Naghabushana K. Nayidu
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  2. Yifang Tan
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Correspondence to Naghabushana K. Nayidu or Margaret Y. Gruber.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table S1. Q-PCR validation of microarray expression patterns. (DOCX 23 kb)

Supplementary Table S2. Heteronuclear NMR spectral data for compounds “a” and “b”. (DOCX 18 kb)

11103_2014_201_MOESM3_ESM.xlsx

Supplementary Table S3. Global differential transcript sets from young seedling leaves of B. villosa relative to B. napus. Data was collected using a custom-designed 95 K B. napus oligoarray detailed in (Trick, et al. 2009). (XLSX 359 kb)

Supplementary Figure S1. 1H and 13C NMR spectral plot for B. villosa alkaloid-like compound “a”. (TIFF 28 kb)

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Supplementary Figure S2. 1D 1H-NMR chemical shifts for compounds “a” and “b”. Arrows indicate the difference chemical shifts between compounds “a” and “b”. (TIFF 78 kb)

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Supplementary Figure S3. Q-PCR of 12 differentially expressed genes validating microarray expression datasets. A portion of this data is also found in Supplementary Table 1. A. Up-regulated genes. B. Down-regulated genes. (TIFF 77 kb)

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Nayidu, N.K., Tan, Y., Taheri, A. et al. Brassica villosa, a system for studying non-glandular trichomes and genes in the Brassicas. Plant Mol Biol 85, 519–539 (2014). https://doi.org/10.1007/s11103-014-0201-1

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  • DOI: https://doi.org/10.1007/s11103-014-0201-1

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