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The endoplasmic reticulum stress induced by highly expressed OsrAAT reduces seed size via pre-mature programmed cell death

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An Erratum to this article was published on 07 May 2013

Abstract

The high accumulation of a recombinant protein in rice endosperm causes endoplasmic reticulum (ER) stress and in turn dramatically affects endogenous storage protein expression, protein body morphology and seed phenotype. To elucidate the molecular mechanisms underlying these changes in transgenic rice seeds, we analyzed the expression profiles of endogenous storage proteins, ER stress-related and programmed cell death (PCD)-related genes in transgenic lines with different levels of Oryza sativa recombinant alpha antitrypsin (OsrAAT) expression. The results indicated that OsrAAT expression induced the ER stress and that the strength of the ER stress was dependent on OsrAAT expression levels. It in turn induced upregulation of the expression of the ER stress response genes and downregulation of the expression of the endogenous storage protein genes in rice endosperm. Further experiments showed that the ER stress response upregulated the expression of PCD-related genes to disturb the rice endosperm development and induced pre-mature PCD. As consequence, it resulted in decrease of grain weight and size. The mechanisms for the detriment seed phenotype in transgenic lines with high accumulation of the recombinant protein were elucidated.

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Acknowledgments

We are grateful to Dr. Masahiro Ogawa for kindly providing the prolamin antibody. This work was supported by the National Scientific Foundation (No. 30671286), the National High-tech R&D Program (863 Program) of China (No. 2011AA100604) and the Major Projects of Genetically Modified Crop of China (No. 2011ZX08001-006).

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, Engineering Research Center for Plant Biotechnology and Germplasm Utilization, Ministry of Education, Wuhan, People’s Republic of China

    Liping Zhang, Daiming Jiang, Jianlei Pang, Rong Chen, Xianghong Wang & Daichang Yang

  2. College of Life Sciences, Wuhan University, Wuhan, People’s Republic of China

    Liping Zhang, Daiming Jiang, Jianlei Pang, Rong Chen, Xianghong Wang & Daichang Yang

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  1. Liping Zhang
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  2. Daiming Jiang
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  3. Jianlei Pang
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  4. Rong Chen
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  5. Xianghong Wang
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  6. Daichang Yang
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Correspondence to Daichang Yang.

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Supplementary table 1 The primers used for Quantitative-PCR

Supplementary Figure 1 The expression profiles of the different storage protein genes at 3, 6, 9 DAP

11103_2013_56_MOESM3_ESM.tif

Supplementary Figure 2 PCD signals were monitored by TUNEL assay in rice seed development cells at 2, 4, and 6 DAP. The red signal indicates DNA, and the green signal indicates DNA fragmentation

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Zhang, L., Jiang, D., Pang, J. et al. The endoplasmic reticulum stress induced by highly expressed OsrAAT reduces seed size via pre-mature programmed cell death. Plant Mol Biol 83, 153–161 (2013). https://doi.org/10.1007/s11103-013-0056-x

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