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Class D and Bsister MADS-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (Triticum aestivum L.)

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Abstract

Homeotic transformation of stamens into pistil-like structures (pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivum L.) that have the cytoplasm of a related wild species, Aegilops crassa. An ectopic ovule differentiates in the pistil-like stamen in the alloplasmic wheat. The SEEDSTICK (STK)—like class D MADS-box gene, wheat STK (WSTK), was expressed in the primordia of ectopic ovules in the pistil-like stamens as well as in the true pistil, suggesting that ectopic ovule formation results from WSTK expression in the pistil-like stamens of alloplasmic wheat. The ectopic ovule is abnormal as it fails to form complete integuments. Based on the expression pattern of WSTK and Bsister MADS-box gene, WBsis (wheat B sister ), we conclude that WSTK plays a role in determination of ovule identity in the pistil-like stamen, but complete ovule development fails due to aberrant expression of WBsis.

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Abbreviations

CMS:

Cytoplasmic male sterility

CS:

Chinese Spring

ORF:

Open reading frame

PCMS:

Photoperiod-sensitive cytoplasmic male sterility

Rf :

Restorer of fertility

N26:

Norin 26

WAG :

Wheat AGAMOUS

WBsis :

Wheat B sister

WSTK :

Wheat SEEDSTICK

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Acknowledgments

We are grateful to the National Bioresource Project—Wheat (NBRP-KOMUGI) for providing wheat materials. This work was supported in part by a Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Science and Culture of Japan (No. 17580009) and from the Fukui Prefectural Government (to K. M.).

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  1. Department of Bioscience, Fukui Prefectural University, Eiheiji-cho, Fukui, 910-1195, Japan

    Kaori Yamada, Tatsunori Saraike, Naoki Shitsukawa, Chizuru Hirabayashi & Koji Murai

  2. Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    Shigeo Takumi

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  1. Kaori Yamada
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  2. Tatsunori Saraike
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Correspondence to Koji Murai.

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K. Yamada and T. Saraike contributed equally to this work.

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Yamada, K., Saraike, T., Shitsukawa, N. et al. Class D and Bsister MADS-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (Triticum aestivum L.). Plant Mol Biol 71, 1–14 (2009). https://doi.org/10.1007/s11103-009-9504-z

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