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Altered expression of wheat AINTEGUMENTA homolog, WANT-1, in pistil and pistil-like transformed stamen of an alloplasmic line with Aegilops crassa cytoplasm

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Abstract

Homeotic transformation of stamens into pistil-like structures, called pistillody, has been reported in some alloplasmic common wheat lines with Aegilops crassa cytoplasm. An alloplasmic line of Chinese Spring ditelosomic 7BS (CSdt7BS) with Ae. crassa cytoplasm lacking the long arm of the chromosome 7B shows pistillody, and the pistils and transformed stamens are sterile due to abnormal ovule development. To elucidate the molecular mechanism of the ovule abnormality, we compared the expression profiles of floral organs between euplasmic and alloplasmic CSdt7BS lines. Two differential display methods of mRNA profiling demonstrated that Ae. crassa cytoplasm largely affects nuclear gene expression profiles of common wheat. Of the differentially expressed genes, a wheat AINTEGUMENTA (ANT) homolog, WANT-1, was preferentially expressed in pistils but not in stamens, and accumulation of the transcript was limited to ovule primordia at the floral organ development stage. In alloplasmic wheat, WANT-1 expression was patchy and weak at the ovule-development stages. On the other hand, no significant difference in gene expression patterns of wheat AGAMOUS (AG) homologs (WAG-1 and WAG-2) was observed between fertile and sterile pistils. These results indicated that alteration of gene expression after initiation of ovule primordia results in abnormal ovule development, and that the aberrant ovule formation is at least partly associated with the weak expression of WANT-1 around ovule primordia in alloplasmic wheat with Ae. crassa cytoplasm.

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Acknowledgements

The EST clone and wheat seeds used in this study were supplied by the National BioResource Project—Wheat (Japan; www.nbrp.jp). This work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan (no. 17780005) to ST.

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Authors and Affiliations

  1. Laboratory of Plant Genetics, Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, 657-8501, Japan

    Kota Mizumoto, Hitoshi Hatano & Shigeo Takumi

  2. Department of Bioscience, Fukui Prefectural University, Eiheiji-cho, Fukui, 910-1195, Japan

    Chizuru Hirabayashi & Koji Murai

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  1. Kota Mizumoto
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  2. Hitoshi Hatano
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  3. Chizuru Hirabayashi
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  5. Shigeo Takumi
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Correspondence to Shigeo Takumi.

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Communicated by K. Schneitz

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Figure S1.

Nucleotide sequence of a full-length cDNA of WANT-1. Underlines indicate two putative AP2 domains. Primer positions for isolation of the WANT-1 ORF are represented by italics. Boxed sequences correspond to primer positions for PCR-RFLP analysis. The putative initiation and stop codons are indicated in boldface (GIF 1028 kb).

Figure S1.

Nucleotide sequence of a full-length cDNA of WANT-1. Underlines indicate two putative AP2 domains. Primer positions for isolation of the WANT-1 ORF are represented by italics. Boxed sequences correspond to primer positions for PCR-RFLP analysis. The putative initiation and stop codons are indicated in boldface (TIF 460 KB).

Figure S2.

Polymorphisms between two homoeologous WANT-1 cDNA sequences. The polymorphisms are categorized into substitution and insertion/deletion, and the number of differences is represented in each nucleotide and deduced amino acid sequence (GIF 354 kb).

Figure S2.

Polymorphisms between two homoeologous WANT-1 cDNA sequences. The polymorphisms are categorized into substitution and insertion/deletion, and the number of differences is represented in each nucleotide and deduced amino acid sequence (TIF 110 KB).

Figure S3.

Schematic representation of polymorphic sites in the WANT-1 genomic region for PCR-RFLP analysis. Exon regions are indicated by black boxes. Three types of PCR fragments (#1, #2, and #3) are generated from total DNA of CS. The insertion and deletion sites are respectively represented by open triangles above and below the thin lines. Vertical lines show nucleotide substitutions. Asterisks indicate polymorphic recognition sites of the restriction enzyme in the label below (GIF 415 kb).

Figure S3.

Schematic representation of polymorphic sites in the WANT-1 genomic region for PCR-RFLP analysis. Exon regions are indicated by black boxes. Three types of PCR fragments (#1, #2, and #3) are generated from total DNA of CS. The insertion and deletion sites are respectively represented by open triangles above and below the thin lines. Vertical lines show nucleotide substitutions. Asterisks indicate polymorphic recognition sites of the restriction enzyme in the label below (TIF 135 KB).

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Mizumoto, K., Hatano, H., Hirabayashi, C. et al. Altered expression of wheat AINTEGUMENTA homolog, WANT-1, in pistil and pistil-like transformed stamen of an alloplasmic line with Aegilops crassa cytoplasm. Dev Genes Evol 219, 175–187 (2009). https://doi.org/10.1007/s00427-009-0275-y

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