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Shared and novel molecular responses of mandarin to drought

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Abstract

Drought is the most important stress experienced by citrus crops. A citrus cDNA microarray of about 6.000 genes has been utilized to identify transcriptomic responses of mandarin to water stress. As observed in other plant species challenged with drought stress, key genes for lysine catabolism, proline and raffinose synthesis, hydrogen peroxide reduction, vacuolar malate transport, RCI2 proteolipids and defence proteins such as osmotin, dehydrins and heat-shock proteins are induced in mandarin. Also, some aquaporin genes are repressed. The osmolyte raffinose could be detected in stressed roots while the dehydrin COR15 protein only accumulated in stressed leaves but not in roots. Novel drought responses in mandarin include the induction of genes encoding a new miraculin isoform, chloroplast β-carotene hydroxylase, oleoyl desaturase, ribosomal protein RPS13A and protein kinase CTR1. These results suggest that drought tolerance in citrus may benefit from inhibition of proteolysis, activation of zeaxanthin and linolenoyl synthesis, reinforcement of ribosomal structure and down-regulation of the ethylene response.

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Acknowledgments

We thank María J. Rodrigo and Lorenzo Zacarías (IATA, Valencia) for the Escherichia coli M15 strain expressing CrCOR15. This work was funded by “Ministerio de Educación y Ciencia” (Madrid, BFU2005-06388-C04-01 and AGL2007-65437-C04-01 grants) and “Consellería de Agricultura, Pesca y Alimentación de la Generalitat Valenciana” (Valencia, “Proyecto Genoma Cítricos”).

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  1. Instituto De Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Camino de Vera s/n, 46022, Valencia, Spain

    Jacinta Gimeno, José Gadea, Javier Forment, Jorge Pérez-Valle, Julia Santiago, María A. Martínez-Godoy, Lynne Yenush, José M. Bellés & Ramón Serrano

  2. Centro de Genómica, Instituto Valenciano de Investigaciones Agrarias, Carretera Moncada- Náquera Km. 4.5, 46113, Moncada, Valencia, Spain

    Javier Brumós, José M. Colmenero-Flores & Manuel Talón

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  1. Jacinta Gimeno
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  2. José Gadea
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Correspondence to Ramón Serrano.

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Gimeno, J., Gadea, J., Forment, J. et al. Shared and novel molecular responses of mandarin to drought. Plant Mol Biol 70, 403–420 (2009). https://doi.org/10.1007/s11103-009-9481-2

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