Abstract
The introduction of genetically modified organisms (GMO) in many countries follows strict regulations to assure that only products that have been safety tested in relation to human health and the environment are marketed. Thus, GMOs must be authorized before use. By complementing more targeted approaches, profiling methods can assess possible unintended effects of transformation. We used microarrays to compare the transcriptome profiles of widely commercialized maize MON810 varieties and their non-GM near-isogenic counterparts. The expression profiles of MON810 seedlings are more similar to those of their corresponding near-isogenic varieties than are the profiles of other lines produced by conventional breeding. However, differential expression of ∼1.7 and ∼0.1% of transcripts was identified in two variety pairs (AristisBt/Aristis and PR33P67/PR33P66) that had similar cryIA(b) mRNA levels, demonstrating that commercial varieties of the same event have different similarity levels to their near-isogenic counterparts without the transgene (note that these two pairs also show phenotypic differences). In the tissues, developmental stage and varieties analyzed, we could not identify any gene differentially expressed in all variety-pairs. However, a small set of sequences were differentially expressed in various pairs. Their relation to the transgenesis was not proven, although this is likely to be modulated by the genetic background of each variety.
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Abbreviations
- cDNA:
-
Complementary DNA
- CRM:
-
Certified reference material
- E:
-
Efficiency
- EBI:
-
European Bioinformatics Institute
- EFSA:
-
European Food Safety Authority
- EU:
-
European Union
- FAO/WHO:
-
Food and Agriculture Organization / World Health Organization
- GM:
-
Genetically Modified
- GMO:
-
Genetically Modified Organism
- IRMM:
-
Institute for Reference Materials and Measurements
- ISAAA:
-
International Service for the Acquisition of Agri-biotech Applications
- mRNA:
-
messenger RNA
- OECD:
-
Organisation for Economic Co-operation and Development
- real-time RT-PCR:
-
Reverse transcription—real-time polymerase chain reaction
- RMA:
-
Robust Multichip Average
- rRNA:
-
ribosomal RNA
- V2:
-
Vegetative two-leaf stage
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Acknowledgements
We specially thank Prof. E. Montesinos (INTEA, UdG) for critically reading of the manuscript. We thank R. Collado (UdG) and J.M. García-Cantalejo (Parque Científico de Madrid) for technical assistance; T. Esteve (CRAG), J. Serra and J. Salvia (E.E.A. Mas Badia) for valuable suggestions. This work was financially supported by the Spanish MEC project with ref. AGL2007-65903/AGR. AC received a studentship from the Generalitat de Catalunya (2005FI 00144).
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Anna Coll and Anna Nadal equally contributed to this work.
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Coll, A., Nadal, A., Palaudelmàs, M. et al. Lack of repeatable differential expression patterns between MON810 and comparable commercial varieties of maize. Plant Mol Biol 68, 105–117 (2008). https://doi.org/10.1007/s11103-008-9355-z
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DOI: https://doi.org/10.1007/s11103-008-9355-z