Abstract
Horsemint (Mentha longifolia L), is wild-growing species, widespread in Eurasia and Africa. The review focuses on its potential utilization as a preservative and flavoring in the food industry based on the polyphenolic and terpenoid composition. Several phenolic antioxidants were detected in horsemint, among which rosmarinic acid may have a key role. Nineteen other acids, and fifty-five flavonoids (six which are de novo) were also identified. The antiradical efficacy in horsemint extract has not yet been adequately justified. Similarly, systematic screening of the flavonoid composition of the species is lacking. Horsemint essential oils possesses an outstandingly wide variability in composition which may serve as basis of special flavoring or antimicrobial agents. The efficacy of horsemint volatiles have been demonstrated against more than twenty microbes. As current literature of horsemint lacks comparable results, the present review provides the broadest and therefore, a critical overview, on its most important secondary compounds and the factors influencing their accumulation.
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Introduction
Mentha longifolia L, horsemint, wild or biblical mint is a perennial herb belonging to the Mentha genus in the Nepetoideae subfamily of Lamiaceae. According to the monography of the genus (Tucker and Naczi 2007), its natural distribution area is the largest among wild-growing Mentha species, covering temperate and mediterranean regions of Eurasia and Africa. (Tucker and Naczi 2007; Sevindik et al. 2017; Sevindik 2018).
This may be evaluated as a sign of adaptivity. The large number of taxa included by the species indicates its genetic diversity. The monography lists 22 subspecies of M. longifolia described from different regions of the world (Table 1).
In Turkey, Iraq, Iran, Pakistan and Arabic countries, leaves or flowering shoots are used as a spice, i.e. for dairy specialties (Tunçturk et al. 2011; Mahmoudi et al. 2012; Ehsani and Mahmoudi 2012), as leafy vegetables, herbal tea and an ethnomedicinal remedy (Ghoulami et al. 2001; Başer et al. 2012; Iqbal et al. 2013; Mikaili et al. 2013; Murad et al. 2016; Sevindik et al. 2017). A recent review of Farzaei et al. (2017) provides ethnopharmacological data in the aforementioned regions, with a wide variety of traditional indications. Beside collection, M. longifolia is reported to be cultivated in Tunisia (Hajlaoui et al. 2009) and its intraspecific taxon M. longifolia var. schimperii syn. ssp. schimperii in Sudan (Younis and Beshir 2011).
In Europe, M. longifolia is far less known and used, contrary to its abundance in wet meadows, forests and ruderal areas. A couple of works have however, been published on analyzing constituents and/or preparations of M. longifolia due to potential industry-related uses (Dudai et al. 2006; Güllüce et al. 2007; Krzyzanowska et al. 2011; Bertoli et al. 2011; Orhan et al. 2012). Beside them, some works are also available on M. longifolia as a medicinal plant, primarily of antiinflammatory and chemoprotective effects (Mimica-Dukić et al. 1996, 1999; Shen et al. 2011; Baris et al. 2011; Vladimir-Knežević et al. 2014). Nevertheless, M. longifolia is less studied as other Mentha species, partially concerning its nonvolatile constituents. Until now it has not been used either industrially or pharmaceutically on a large-scale. However, it seems to be a cheap and prosperous additive in numerous products. A promising application may be the usage of M. longifolia polyphenols in the food industry as antioxidants (AOs) to increase shelf life. This potential use may be considered with regard to the high demand of plant-originated antioxidants (AOs) and in parallel, health concerns due to some synthetic phenolic AOs (Shahidi and Ambigaipalan 2015). Another alternative may be utilization of the volatiles of selected M. longifolia chemotypes against foodborne microbes, or as flavoring agents. In the present review, these two potential ways of utilization of this adaptive species, having a large tolerance for various habitats, in different preparations are in focus. Therefore, a detailed survey was carried out on the respective secondary compounds and a thorough evaluation is presented.
Materials and search strategy
Beside the comprehensive review Labiatae flavonoids and their bioactivity (Ulubelen et al. 2005) as a starting point, studies on M. longifolia and related species evaluated here were primarily obtained from electronic databases, namely SpringerLink, ScienceDirect, Journal of Agricultural and Food Chemistry, JEOR, Wiley Online Library, Taylor and Francis and MDPI. PubMed, Google Scholar and ResearchGate were used to search reliable but less known sources like the study of Jahan et al. (2001) on a novel flavone detected from ML. To check the background of journals providing some of the latter, Scimago was used. References in available publications were also screened in further sources like dissertations or less cited articles. One example was the study on phenolics of M. x piperita (Guédon and Pasquier 1994) referring to the earliest available work on M. longifolia flavonoids (Bourwieg and Pohl 1973) as a nowadays less known source to exploit. Further references considered to be necessary (e.g. studies dealing with structure—AO activity relationships of flavonoids), were searched also at the above mentioned databases. In the present review the cited data on concentrations of phenolics in M. longifolia will be given both in the original measuring units as they were published and also, in the majority of cases, in mg/kg dry plant material (mg/kg dp) for the sake of better comparability.
Cinnamic acid derivatives in ML
Rosmarinic acid
Rosmarinic acid or ‘Labiatae tannin’ (further: RA) is the caffeoyl ester of caffeic acid and 3′,4′-dihydroxyphenyllactic acid. Accumulation of RA is characteristic in the Nepetoideae subfamily of the Lamiaceae family. Petersen and Simmonds (2003) summarize RA as adstringent, AO, antiviral, antimutagen and antiinflammatory agent. Investigations on M. longifolia phenolics have predominantly been focusing on RA as a potent AO (Dudai et al. 2006; Fialová et al. 2008; Krzyzanowska et al. 2011; Patonay et al. 2017) antiinflammatory (Shen et al. 2011) and anticholinesterase (Vladimir-Knežević et al. 2014) molecule. The available quantitative data dealing with RA content of M. longifolia is summarized in Table 2. However, as large differences are observable in the investigated drug types and plant developmental stages (if defined), comparison of data can not be totally adequate. The most thorough publication on RA and caffeic acid content of M. longifolia is the work of Dudai et al. (2006) being the only one analyzing large sample numbers of M. longifolia for any phenolics. Results represent the highest RA content available in the literature of M. longifolia, covering 20–80 mg/g dp. (20,000–80,000 mg/kg dp). On the other hand, a recent work (Park et al. 2019) gives unconventionally low RA concentration (18.68 μg/g dp. viz. 18.68 mg/kg dp) from a single M. longifolia sample of undefined phenophase. In general, there is a relatively large variability in RA concentrations of M. longifolia mentioned by different authors, and they seem to be determined not only genetically but might be the result of differences in the plant developmental stage, harvest time (Fialova et al. 2008), cultivation technics, drug types (Krzyzanowska et al. 2011), extraction methods or other factors (Table 2).
Other phenolic acids, esters and phenylpropanoid volatiles
Beside RA, further phenolics have been detected in M. longifolia samples. Table 3 summarizes their concentrations. Nepetoidin A and B are reported to be present in M. longifolia (Grayer et al. 2003) as a chemotaxonomical marker of Nepetoideae plants. Salvianolic acid L and dedihydro-salvianolic acid was detected by Krzyzanowska and co-workers (2011). It may be important to note that m/z data and UV maxima (283.3, 344.4 nm) of dedihydro-salvianolic acid were provided in this study, but molecular structure of a compound with this name was found neither in PubChem, PhenolExplorer, Human Metabolome Database or NIST Webbook, nor in literature. Hexacosyl ferulate and bis-2-ethylhexil-benzene 1,2-dicarboxylate were reported from Mentha longifolia L ssp. noëana sampled in Turkey (Ertaş et al. 2015). This is the first report of them from M. longifolia, thus, based on this single reference it is impossible to evaluate the frequency and level of their concentration in horsemint. In general, data of minor phenolic acids in this species outline a rather wide variablity, but unfortunately there is hardly any data about the influencing factors of the accumulation of them until now.
Flavonoids in horsemint
Table 4 summarizes structural information of flavonoids reported in Mentha longifolia.
Flavanones
Flavanones (’citrus flavonoids’) are usually a dominant or a major flavonoid subclass in mints, together with flavones (Pereira and Cardoso 2013). Regarding M. longifolia, data about them is sporadic and a majority of the references report only the presence of these compounds (eriocitrin, hesperidin and narirutin) without quantitative data. The spectrum of flavanones include relatively widespread compounds (Table 4), but a special flavanone, 4′-methoxy-naringenin-7-O-fucopyranosil-1 → 6-glucoside or longitin, reported from a Pakistani sample may be mentioned as a novelty (Ali et al. 2002).
Flavones
Table 5 shows the available quantitative data of flavones in M. longifolia. This flavonoid subclass shows a very wide variability in ML samples. Among them, there are some compounds which have not been known before and detected especially in ML for the first time. A novel aglycone with unconventional substitution pattern, 5,7,4′-trihydroxy-6,2′,3′-trimethoxy-flavone, was detected by Ghoulami et al. (2001) from Morocco. Besides, a low concentration of another new aglycone, 5,8,4′-trihydroxy-6,7,3′-trimethoxy-flavone was found by Jahan et al. (2001), from Pakistan. Exploration of three previously unknown tricetin derivatives in a M. longifolia sample from Saudi Arabia is reported by Sharaf et al. (1999). According to the authors, it is the first report on flavones bearing trisubstituted B ring in the whole Lamiaceae family. However, the occurrence of the mentioned special flavones in horsemint seems to be supported only by the cited single reference.
Among flavone glycosides, cynaroside has been detected repeatedly, (Table 4) although its concentration is low (or not provided) (Table 5). In some cases, the sites of the glycosidic bonds are not designated, thus the exact glycoside molecule remains questionable, e.g. luteolin-glucorhamnoside and luteolin-glucuronides in study of Krzyzanowska et al. (2011). It can be established, that the sporadic data about flavone-7-O-glycosides as summarized in Tables 4 and 5 do not seem to represent strong support for the universal and frequent accumulation of them in horsemint, although these ingredients have been frequently described in other mint species (Guédon and Pasquier 1994; Areias et al. 2001; Damien-Dorman et al. 2003a, b; Koşar et al. 2004).
Flavonols
Although the previous reviews (Pereira and Cardoso 2013; Mikaili et al. 2013; Farzaei et al. 2017) do not deal with this subclass in detail when discussing the flavonoids of M. longifolia, the available literature shows that flavonols may frequently be present in this species. Quercetin and kaempferol together with their glycosides are most often reported from M. longifolia (Table 4.). The concentration ranges are variable, like in the case of rutin: 0.822 mg/100 g dp (Benedec et al. 2013) or 11 66 mg/100 g dp (Park et al. 2019). Flavonol-rich samples were reported from Serbia (Stanislavljević et al. 2012), Hungary (Patonay et al. 2017) and Korea (Park et al. 2019). Interestingly, Stanislavljević et al. (2012) reported astragalin to be the dominant flavonol constituent of a M. longifolia charge (61.36 mg/g extract calculated with yield: 7118 mg/kg dp).
It seems, that the actual amount of flavonoid compounds in the drug may be influenced by drying method (Stanislavljević et al. 2012) or other postharvest treatments like heating the fresh plant material (Stocker and Pohl 1976). These questions may need a further study.
Antiradical and antimicrobial properties of phenolics occurring in horsemint
Rosmarinic acid plays an important role in the antioxidant properties of M. longifolia extracts. Dudai and co-workers established a tight correlation (R2 = 0.38) between rosmarinic acid content and results of DPPH assay. However, Fialová and co-workers (2008) suggest, that other constituent(s) than this may play a role in the radical scavenging activity of M. longifolia as the maxima of THD, TF and antiradical activity do not coincide with the maxima of RA content. Interestingly, the concentration of caffeic acid does not seem to correlate with results of DPPH assay (R2 = 0.0119) contrary to its known AO efficiency (Koşar et al. 2004; Csepregi et al. 2016). Grayer et al. (2003) observed nepetoidin B to be a stronger AO than gallic acid in DPPH assay.
As Table 4 shows, a significant proportion of the flavonoids detected in ML are the 7-O-glycosides. Although they are frequent in antioxidant-rich species of plant families e.g. Lamiaceae, Apiaceae, Asteraceae, their AO properties are less known in comparison with 3-O-glycosides (Csepregi et al. 2016). Therefore, the antiradical abilities of 7-O-glycosides may principally be outlined using studies of structure–activity relationship. Bors and co-workers (1990) studied the kinetics of various flavonoids against OH·, N ·3 and tert-butoxyl radicals demonstrating that the key of the AO activity of flavonoids towards radicals is the ability to form a longlife secondary aroxyl radical which could take part in recombinations. In this consideration, authors outlined the necessary structural traits providing better delocalization of the unpaired electron and in consequence, stability of aroxyl radicals. These are the followings (1) free ortho-dihydroxy group at B ring (catechol moiety) (2) the free –OH group at C3 (3) double bond at C2-C3 and carbonyl on C4, because of conjugation (4) additionally, presence of free –OH groups at C5 and C7. Later, studies ranking flavonoids on TEAC (Rice-Evans et al. 1996; Csepregi et al. 2016) and DPPH assays (Burda and Ołeszek 2001; Csepregi et al. 2016) modified this idea. Catechol moiety was repeatedly observed to play a key role in AO properties, followed by free C3–OH. The latter was recently observed to be tightly and significantly correlated with activity in TEAC and FRAP assays but loosely coupled to the activity in DPPH and Folin-Ciocalteu’s assay (Csepregi et al. 2016). The C2–C3–C4 system was reinforced to function only in combination with free catechol moiety and/or C3–OH (Wen et al. 2014; Csepregi et al. 2016). Based on these considerations, some flavonoid-7-O-glycosides detected in ML may deserve attention. Thus, luteolin-7-O-glycosides may be predicted as active against some radicals as rutin as they have catechol moiety and C2-C3-C4 conjugation but free C3–OH is absent. A ranking of flavonoids by activity against DPPH (Burda and Ołeszek 2001) supports this idea. Here, rutin showed 90.9 IC% and cynaroside 87.6 IC%. Luteolin itself was also observed to show antiradical activity stronger than of BHT on DPPH assay but weaker efficacy on ORAC (Wen et al. 2014), suggesting that the lack of C3–OH might decrease this kind of AO activity. On the other hand, eriodyctiol and 7-O-glycosides may be considered as stronger antiradical agents than other flavanones of ML because only they have a free catechol group. Damien-Dorman and co-workers (2003a, b) declared, that mints richest in eriocitrin and rich in RA showed the highest activity against DPPH and OH·. Their further study (Koşar et al. 2004) demonstrated a high correlation between DPPH antiradical activity of Mentha extracts and concentration of caffeic acid, rosmarinic acid, lonicerin, eriocitrin and an undefined luteolin-7-O-glycoside. Antiradical activity of luteolin-5-O-glycosides like galuteolin may be supposed to be similar to 7-O-analogues because of the presence of free catechol moiety and C2–C3–C4 conjugation. Naringenin and apigenin derivatives however, as it may be expected based on their structure, did not show this response.
It must be emphasized, that synergistic effects between some flavonoids and/or flavonoids and caffeic acid derivatives may occur, depending on the ratio of concentration and their redox potential and the presence of catechol moiety in the case of flavonoids (Freeman et al. 2010; Reber et al. 2011; Ołszowy-Tomczyk 2020). A very recent long-needed review of Ołszowy-Tomczyk (2020) called attention to the mutual effects of plant phenolics in binary mixtures. The detailed data collected by the author shows that there are some cases when synergistic or additive effects were reported between polyphenols e.g. between rosmarinic acid and quercetin in the case of AAPH induced oxidation; between chlorogenic acid and hesperidin, also between p-coumaric acid and quercetin in ORAC assay. On the other side, no antagonistic effect was reported to rosmarinic acid and flavonoids except an observation on FRAP assay of rutin and rosmarinic acid (Hajimehdipoor et al. 2014). Although an extract is much more complex than a binary mixture, synergistic or antagonistic effects may be considered when the background of antioxidant properties of a ML extract is studied.
Beside the plant material itself, studies rarely focused on other factors which might influence the AO properties of ML extracts. Fialová and co-workers (2008) proved that ML show higher AO activity, THD and TF in July than in September (DPPH EC50 in July 24.60 μg/mL, in September 45.20 μg/mL). Further studies are needed in this respect.
Focusing on the food preservative utilisation of M. longifolia, beyond the AO activity of phenolic compounds, the activity against bacteria or fungi causing food spoilage and/or foodborne diseases may be taken into account. Akroum and co-workers (2009) established that isoquercitrin in M. longifolia showed the strongest growth inhibitory effect against B.cereus, B.subtilis, S.aureus, E.coli and P.aeruginosa (MIC = 0.03–0.09 μg/mL). Synergism among these molecules was observed. Other polyphenols of ML may also be potential antimicrobial agents, as documented in in vitro studies in the case of other species, like apigenin (Basile et al. 1999; Metsämuuren and Sirén 2019), luteolin (Wen et al. 2014) and nepetoidins (Grayer et al. 2003).
The volatile composition of horsemint
Essential oil (EO) content of horsemint and classification of its constituents
According to recent data, volatile components accumulate in M. longifolia in a range of 0.5–1% dry weight (Hajlaoui et al. 2009; Sharopov et al. 2012; Iqbal et al. 2013; Llorens-Molina et al. 2015; Kapp 2015). However, earlier studies report significantly higher EO contents, up to 1.6–2.8% from Eastern Crete (Karousou et al. 1998) and 3.8% from Sinai (Fleisher and Fleisher 1991). This wide interval of EO contents may be in part coupled to sampling methods and the varying phenological phase or organic composition of the plants (EO yield of the plant is recently observed by Llorens-Molina et al. (2020) to reach its maximum in advanced flowering stage). Anyhow, the different experimental conditions make a proper evaluation difficult. Illustrating this, the analysed sample types include flowering shoots (Karousou et al. 1998), shoots at the end of flowering, or seed ripening stage (Baser et al. 1999) or even leaves separated from the stems (Orav et al. 2013).
Volatiles of ML show extraordinary wide variability, involving multiple metabolic pathways. Based on works of Başer and co-authors (1999; 2012), volatile terpenes of M. longifolia could be perspicuously grouped by structure (Figs. 1, 2). These groups and their important representants are presented below.
Open-chain monoterpenes
Linalool (Mimica-Dukic and Bozin 2008) and linalyl acetate may be present in concentrations above 10% of ML EO (Al-Okbi et al. 2015), although they do not appear in all M. longifolia samples. Thus, they may not be considered as universal constituent of the species. Myrcene was also reported in concentration around 10% in samples from Lithuania (Venskutonis 1996).
Limonene and its 2-oxo derivatives
Carvone, dihydrocarvone, cis- and trans-carvyl acetate, cis- and trans-dihydrocarveol frequently appear in EOs of M. longifolia (Başer et al. 1999; Sharopov et al. 2012; Mimica-Dukic and Bozin 2008). 55–66% carvone was present in the EO of the samples from Crete (Karousou et al. 1998) while 50–65% carvone was reported from Iran, former Yugoslavia, France, Estonia and Tajikistan (Sharopov et al. 2012; Kapp 2015).
Limonene 3-oxo derivatives
Piperitone, the two piperitone oxide isomers, piperitenone and piperitenone epoxide are typical in the EO (Başer et al. 1999; Aksit et al. 2013). Pulegone appears frequently, too. This volatile, a major component also of the pennyroyal (M. pulegium), bears an unpleasant aroma and is considered to be toxic. Target human organs are suggested to be the liver and kidney which may be damaged via reactive metabolites in the case of long-term consumption (EPA/HMPC/138386/2005 Rev 1) (European Medicines Agency, Committee on Herbal Medicinal Products (HMPC) 2016). The EU directive EC1334/2008 (EEC 2008) declares that pulegone and menthofurane are limited to max. 20 mg/kg in general foodstuff, 200 mg/kg in mint/peppermint flavoured confectionery, and 100 mg/kg in chewing gums. Proportion of pulegone in a ML EO varies between 20 and 85% (Fleisher and Fleisher 1991; Baser et al. 1999; Ghoulami et al. 2001; Güllüce et al. 2007; Sharopov et al. 2012; Kapp 2015). Further representants of limonene 3-oxo derivatives in M. longifolia are menthone, isomenthone, menthofurane (Mimica-Dukic and Bozin 2008; Kapp 2015) and an accession rich in menthol is also reported (Llorens-Molina et al. 2017). Besides, Ali and co-workers (2002) report from the Pakistani sample mentioned above, a novel chlorinated limonene-3-oxo ketone. It is 1-hydroxy-2-chloromenthone or longifone.
Other cyclic monoterpenes
This group includes terpinen-4-ol, α-terpineol, α-terpinylacetate, eucalyptol, borneol, trans-sabinene hydrate, thymol etc., which were detected as major ingredients of ML EO in just a few cases. Alpha-terpinyl acetate as a main compound in an EO was described independently from Northern Turkey (Baser et al. 1999), Jiloca basin in Spain (Llorens-Molina et al. 2015), and (Kapp 2015). In the Turkish sample the terpinyl ester was present in 42% of the EO, and in the Estonian oil in 48%. The samples from Spain (18 individuals) showed somewhat lower proportion (39%) of the ester. From Serbia, a unique EO composition was described with presence of thymol (13%) together with its precursors γ-terpinene (5%) and p-cymene (14%) accompanied by eucalyptol (7%), however without the typical limonene-derived ketones (Mimica-Dukić et al. 1993).
Sesquiterpenes
The majority of sesquiterpenes has been detected in EOs of horsemint as minor component except β-caryophyllene, caryophyllene oxide and germacrene D which are regularly demonstrated as major ingredients (Mimica-Dukić et al. 1993; Başer et al. 1999; Sharopov et al. 2012; Iqbal et al. 2013; Llorens-Molina et al. 2015; Kapp 2015). Their proportions in the EO make up 2-10%, however, in a Turkish sample of M. longifolia L ssp. typhioides var. typhioides 29% caryophyllene oxide and 12% β-caryophyllene were determined (Başer et al. 1999). Besides, a major unknown was also detected by Başer and co-workers (2012) in samples from Marmara region (Turkey). This compound, probably a sesquiterpene is characterized by a GC retention index RI = 2209. It was present in the EO of a single M. longifolia L ssp. longifolia oil (35% of EO) and in six M. longifolia L ssp. typhioides var typhioides oils (between 6-35%).
Chemotypes of horsemint: open questions
Based on the mentioned varying main compounds of the EO, references declare the presence of different chemotypes. According to Mimica-Dukic and Bozin (2008) the wide diversity of EOs of wild-growing mints is observable in contrary to the relative stability of the composition of cultivated spices. Others authors conclude that EO composition of ML is highly variable even among the wild growing mints (Németh-Zámboriné 2015a) In spite of this, according to our knowledge, no summarizing survey or review of this partial area of the phytochemistry of M. longifolia has been published until now. Here, three larger typologies are considered. Başer and his group (1999) provided EO compositions and typology of Turkish (Aegean region) samples of two ML taxa. From M. longifolia ssp. longifolia (18 samples) five chemotypes were determined: 1() rich in piperitone oxides (2) linalool-rich or linalool-eucalyptol type (3) type based on carvone or carvone and β-caryophyllene (4) type rich in isomenthone (5) other compositions: one α-terpinyl acetate based sample and another rich in terpinen-4-ol and trans-sabinene hydrate. M. longifolia ssp. typhioides var. typhioides (19 samples) have been classified into six chemotypes (1) rich in piperitone oxides (2) linalool-rich (3) carvone-rich (4) rich in trans-sabinene hydrate (5) type based on menthone or menthone/trans-piperiton-oxide (6) EOs based on trans-piperitone oxide/β-caryophyllene or trans-piperitone oxide/β-caryophyllene oxide. Another typology is provided by Mimica-Dukic and Bozin (2008) who distinguish nine chemotypes (signed as types I to IX) of the genus based on surveying both cultivated and wild-growing mint species. M. longifolia s.l. is present in five of these chemotypes: II, rich in linalool and/or linalyl acetate; III, based on carvone or dihydrocarvone; IV, dominated by piperitone or piperitenone; V, piperitone oxides or piperitenone epoxide; IX, menthone, isomenthone or menthol (isomers) as main constituents. In this classification, the chemotype V group contains only M. longifolia and no other Mentha taxa were placed here. Interestingly, no thymol—para-cymene chemotype of M. longifolia is mentioned, although it was reported by the same authors earlier (Mimica-Dukić et al. 1993). Finally, Sharopov and co-authors (2012) list fourteen chemotypes of M. longifolia as the most important ones. This classification is supported by experimental data of M. longifolia samples collected from at least one, but usually 3-8 habitats. The mentioned chemotypes, marked by their key component are as follows: piperitenone epoxide; piperitone oxides; piperitone; isopiperitenone; piperitenone; carvone; trans-dihidrocarvone; pulegone; menthone; isomenthone; menthofurane; menthol; eucalyptol; borneol. Authors note that both EO composition and morphological traits of M. longifolia are highly diverse without mentioning any correlation between chemical and morphological traits.
Comparing the above mentioned three typology, carvone, piperitone, piperitone with its oxides can be established as the basis and the most widespread monoterpenes of ML chemotypes. Other studies mentioning different chemotypes of this species are scarce but recent works report new chemotypes too. A menthofurane rich accession of M. longifolia L ssp. polyadena from South-Africa is described by Viljoen et al. (2006). Three novel types in Teruel region, Spain have been explored via careful sampling of chemotaxonomically heterogeneous populations. These were a cis-sabinene hydrate/terpinen-4-ol, a α-terpinyl acetate/carvyl acetate (Llorens-Molina et al. 2015) and an α-terpineol acetate/8-acetoxy carvotanacetone type (Llorens-Molina et al. 2020) respectively.
Antimicrobial properties of horsemint volatiles
Beside flavour and aroma, the EO might contribute to the preservation of food products. The most comprehensive study (Güllüce et al. 2007) on M. longifolia EO rich in limonene-3-oxo compounds provide the antimicrobial activity against 15 species of molds and 14 strains of bacteria, and also against C. albicans. This data is highly valuable because most of the studies work with a far lower number of microorganisms and/or do not provide strain numbers. The tested EO contained cis-piperitone oxide (18.4%), pulegone (15.5%), piperitenone oxide (14.7%), menthone (7.9%), isomenthone (6.6%), trans-piperitone oxide (4.1%) and in lower (1-5%) proportions limonene-2-oxo volatiles, accompanied by 6.6% thymol. MIC of this EO was lower or equal with the values of control antibiotics against the majority of the tested bacteria (except Streptococcus, Pseudomonas, Enterobacter and Brucella spp). Good anticandidic activity and efficacy against Fusarium spp. was also observed. Based on this finding, authors propose utilization of M. longifolia essential oil as a preservative.
Other constituents of the ML oils, like limonene and its 2-oxo derivatives were also demonstrated to show moderate antibacterial activity on a wide range of pathogens, including foodborne ones (e.g. E. coli, P. aeruginosa, Enterobacter sp.- strain numbers not provided), (Oumzil et al. 2002). The study of Aggarwal et al. (2002) demonstrated the activity of S(-)-carvone, being present frequently in M. longifolia, was as effective against K. pneumoniae and Candida. The antimicrobial activity of limonene-3-oxo-ketones and their epoxides, together with the mint oils characterized by them are rather frequently studied in some cases due to their potential preservative properties. Studies on pulegone, piperitenone, piperitone and epoxides isolated from Mentha (Oumzil et al. 2002) or Satureja species (Tolossa et al. 2007) show that pulegone possesses strong antimicrobial activity. However, as the use of pulegone is limited, its direct utilisation in the food industry does not have much potential. The EO of the thymol-paracymene chemotype from Serbia (Mimica-Dukić et al. 1993) was observed to show considerable activity against B. subtilis, S. aureus, C. albicans and A. niger. A review of the antimicrobial activity of EO is given by Mikaili et al. (2013), with data on decreasing antibiotic resistance of food-borne bacteria together with remarkable effects against moulds, pathogen fungi and protozoas. Ehsani, Mahmoudi and co-workers (2012) demonstrated the preservative effects of M. longifolia EO (with main components pulegone, eucalyptol, menthofurane and isopulegone) in Iranian white-brined cow cheese. The combination of 150 ppm EO and a probiotic bacterium (L. casei) showed a significantly better preservative effect against the dairy-borne pathogens L. monocytogenes and S. aureus, than any of the treatment alone. According to authors, the limiting factor of the EO concentration in the cheese may be its influence to organoleptic properties. In our opininon, the high proportion of pulegone isomers and menthofurane in the EO might also be dangerous.
Concluding remarks
Among the phenolic compounds of M. longifolia, the AO value of rosmarinic acid has been declared frequently. Nevertheless, a part of the available data brings up the question if it is really the only or maybe the most important constituent of strong antiradical properties of the ML extracts. It was demonstrated, that the plant contains a couple of caffetannins and 55 various flavonoids, primarily flavones. Considering the relations of structure and antiradical activity, three groups of flavonoids may deserve attention, i) luteolin-7-O-glycosides like lonicerin and cynaroside, ii) eriodyctiol derivatives (eriocitrin) and iii) derivatives of quercetin, among which rutin is as frequently reported from M. longifolia samples as cynaroside. Rutin content was found to be in significant correlation with the FRAP activity of M. longifolia (Patonay et al. 2017). Park et al. (2019) also observed strong antioxidant activity to their rutin-dominated M. longifolia sample. Further studies are suggested to determine if higher concentrations of quercetin derivatives are really universal characteristics to this species as it was described in some references above.
Unfortunately, well-established conclusions on the available literature data are facing difficulties arising primarily because of methodological problems. Analysis, partially on nonvolatile compounds, are often made on a single batch of questionable representativeness of M. longifolia. Bulk samples are unable to represent the real chemical variability of any population and repetability of the results is also hardly possible. Comparison of published results is aggravated through the missing definition of plant part and phenophase at collection, too. Thus, in lack of representativeness and detailed description of sampling methods, separate references are unable to confirm either the universal appearance of any components or the background of the detected differences. It may be proposed to screen flavonoids of M. longifolia on a wider range of samples instead of single charges. It seems to be necessary to detect also both the biotic and environmental factors which might influence accumulation of these compounds.
During optimalization of industrial uses, the effective solvent of polyphenols of M. longifolia should also be determined. In general, polar extraction results in high AO activity (Mikaili et al. 2013), nonpolar solvents such as hexane or DCM are not effective (Iqbal et al. 2013). A recent study of extractability of M. longifolia, performed by our team on 36 samples, proposes to use water–ethanol with a 3:7 mixture which makes it possible to avoid the toxic methanol in food products (Patonay et al. 2019). The utilization of aqueous waste of EO hydrodistillation was also described as a potential useful way to gain polyphenolic antioxidants of Mentha spp. (Damien-Dorman et al. 2003a, b; Koşar et al. 2004; Shen et al. 2011).
On preservative efficacy of M. longifolia drug or nonvolatile extracts in the food matrix, no published data was found. In case of other mint species, some results are available. The drug of M. spicata in a dairy dessert under thermal treatment, inhibited lipid peroxidation (Bandopadhyay et al. 2008) with similar efficacy as tert-butyl-hydroquinone. In a highly different matrix, namely a whole raw fish, a M. arvensis ethanolic extract was able to increase shelf life by inhibiting lipid peroxidation and release of biogenic amines (Viji et al. 2015). Although these studies do not determine the constituents in the background of the preservative effect, it could be supposed, that phenolics were effective in inhibiting peroxidation based on their radical scavenging properties detailed above. These results allow us to anticipate that a standardized, deodorized M. longifolia extract rich in polyphenols may be a cheap and effective inhibitor of lipid peroxidation and coupled oxidative deteriorations in some sensible types of foodstuff, i.e. in dairy, meat or fish products.
Volatiles of M. longifolia show an extraordinarily wide variability. Because of the complexity of data and eventual contradictions, it can be established, that the chemotaxonomic investigation on M. longifolia needs further thoroughl study. It seems, that the geographical habitat is not closely connected to the abundance of any chemotype and the populations may be heterogenous in contrary to the primarily vegetative propagation behaviour of the plant (Llorens-Molina et al. 2015). To the contrary of some comprehensive studies, a well established definition of M. longifolia chemotypes is still lacking as chemical variability may have several backgrounds (Németh-Zámboriné 2015b). A more adequate knowledge on the occurrence and stability of chemotypes and those of the EO composition may encourage the utilization of the desired types, primarily those free of pulegone as being a potentially cheap source of flavorings. The rich spectrum of volatiles also enables the selection of strains or clones with different aroma characters (e.g. carvone-rich: spearmint like, linalool-rich: reminiscent to lavender, etc.). Beside the phenolics, EO of M. longifolia might contribute to the preservation of food products, too. Evaluation and comparison of data dealing with antimicrobial activity of M. longifolia volatiles is, however aggravating due to the wide and varying spectrum of the investigated microbiota strains and extraction methods as well as the missing details on the enantiomers of the investigated volatiles (Oumzil et al. 2002). Chemotypes rich in piperitone, piperitenone and correspondent oxides, might have a great value as antimicrobial agents against numerous food-borne pathogens. Usage of pulegone-rich EOs should be avoided because of the toxicity of this component.
Abbreviations
- AAPH:
-
2,2′-Azobis(2-amidinopropane) dihydrochloride
- AO:
-
Antioxidant
- DCM:
-
Dichloromethane
- dp:
-
Dry plant material
- DPPH:
-
2,2′-Diphenyl pycrylhydrazyl
- EC50 :
-
Effective concentration-50
- EO:
-
Essential oil
- FRAP:
-
Ferrous reducing activity
- IC%:
-
Inhibitory concentration in percentage
- MIC:
-
Minimal inhibitory concentration
- RA:
-
Rosmarinic acid
- TAC:
-
Total antioxidant capacity
- TF:
-
Total flavonoid content
- THD:
-
Total hydroxycinnamic acid content
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Acknowledgements
Authors gratefully acknowledge the support of the Grant EFOP-3.6.1-16-2016-00001‚ ‘Complex improvement of research capacities and services at Eszterhazy Karoly University’ by the European Social Fund, and by the Ministry for Innovation and Technology (Hungary) within the framework of the Higher Education Institutional Excellence Program (NKFIH-1159-6/2019) in the scope of plant breeding and plant protection researches of Szent István University.
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Patonay, K., Németh-Zámboriné, É. Horsemint as a potential raw material for the food industry: survey on the chemistry of a less studied mint species. Phytochem Rev 20, 631–652 (2021). https://doi.org/10.1007/s11101-020-09718-0
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DOI: https://doi.org/10.1007/s11101-020-09718-0