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Fluorescence imaging of light acclimation of brazilian atlantic forest tree species

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Photosynthetica

Abstract

In the pursuit of knowledge on the biological behavior of Brazilian Atlantic Forest tree species, this study evaluated the susceptibility of the light-demanding species, Schinus terebinthifolia Raddi., Pseudobombax grandiflorum (Cav.) A. Robyns and Joannesia princeps Vell., and of the shade-tolerant species, Hymenaea courbaril L. var. stilbocarpa and Lecythis pisonis Camb, to photoinhibition and acclimation capacity. These species were first cultivated under two irradiance conditions, I20 (20% direct sunlight radiation) and I100 (all-sky or direct sunlight) and then transferred from I20 to I100. The effects of the sudden increase in light radiation intensity on photosynthetic activity were then evaluated through chlorophyll (Chl) fluorescence imaging, HPLC xanthophylls analysis, and cell membrane lipid peroxidation measurements. Light-demanding species were found to present a higher photochemical efficiency and higher acclimation capacity under high light irradiance than shade-tolerant species. The higher photoinhibition tolerance observed in light-demanding species was associated to their higher capacity for photochemical dissipation and dissipation of excess excitation energy via the xanthophyll cycle, leading to a lower ROS generation. The obtained results suggested that a knowledge of acclimation capacity, by means of Chl fluorescence imaging yields, is a useful indicator of species successional grouping.

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Abbreviations

A:

antheraxanthin

DEPS:

de-epoxidation state

F:

transient fluorescence

F0 :

dark fluorescence yield

Fm :

maximum fluorescence yield after dark adaptation

Fm′:

maximum fluorescence in the light-adapted state

Fv/Fm :

maximum quantum yield of PSII

HPLC:

high performance liquid chromatography

LHCs:

light-harvesting complexes

MDA:

malondialdehyde

V:

violaxanthin

Y(II):

effective quantum yield of PSII

Y(NO):

quantum yield of nonregulated nonphotochemical energy dissipation in PSII

Y(NPQ):

quantum yield of regulated nonphotochemical energy dissipation in PSII

Z:

zeaxanthin

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Universidade Federal de Viçosa (DBV/UFV), Av. PH Rolfs, s/n, Viçosa, MG 36571-000, Brazil

    L. Dos Anjos, M. A. Oliva & K. N. Kuki

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  1. L. Dos Anjos
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  2. M. A. Oliva
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  3. K. N. Kuki
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Correspondence to L. Dos Anjos.

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Acknowledgements: The authors would like to thank Dr. Fábio Murillo DaMatta for providing the xanthophyll pigments and Dr. Raimundo Santos Barros for his permission to use their HLPC device. Our sincere thanks also go to the following institutions: CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and FAPEMIG (Fundação de Amparo à Pesquisa de Minas Gerais) for their invaluable financial support, and finally to Vale do Rio Doce Company for providing the seedlings used.

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Dos Anjos, L., Oliva, M.A. & Kuki, K.N. Fluorescence imaging of light acclimation of brazilian atlantic forest tree species. Photosynthetica 50, 95–108 (2012). https://doi.org/10.1007/s11099-012-0018-6

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