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Effect of gamma radiation on mutant induction of Fagopyrum dibotrys Hara

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Photosynthetica

Abstract

Agronomic traits, photosynthetic pigments, gas exchange, and chlorophyll (Chl) fluorescence parameters of red stem buckwheat (Fagopyrum dibotrys Hara) mutants induced by γ-radiation were compared with green control at seedling stage. Plant height, number of first-class branches, and rhizome biomass were inhibited significantly (p<0.01). Chl a, Chl b, and Chl a+b contents decreased with elevated dose of γ-rays, while increasing carotenoid content indicated that buckwheat was capable of adjusting to the radiation damage. Decrease in net photosynthetic rate was the result of both stomatal and non-stomatal limitations. Fluorescence parameters, such as F0, Fm, Fv/Fm, Fv/F0, ΦPS2, electron transport rate, and photochemical quenching declined significantly (p<0.01) as compared with control due to photoinhibition, while non-photochemical quenching increased to enhance thermal dissipation. Lower parameters implied that leaf tissue was damaged significantly by high dose of γ-radiation and therefore leaf senescence was accelerated.

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Abbreviations

Chl:

chlorophyll

Car:

carotenoids

PS2:

photosystem 2

ETR:

electron transport rate

F0 :

minimum fluorescence

Fm :

maximum fluorescence

Fv :

variable fluorescence

Fv/Fm :

maximum photochemical efficiency

LHCP:

light-harvesting chlorophyll-protein complexes

PS:

photosystem

qP, NPQ:

coefficients of photochemical and non-photochemical quenching

ΦPS2 :

actual photochemical efficiency of PS2

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, 100094, Beijing, P.R. China

    C. F. Jia & A. L. Li

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  1. C. F. Jia
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  2. A. L. Li
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Correspondence to A. L. Li.

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Jia, C.F., Li, A.L. Effect of gamma radiation on mutant induction of Fagopyrum dibotrys Hara. Photosynthetica 46, 363–369 (2008). https://doi.org/10.1007/s11099-008-0066-0

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  • DOI: https://doi.org/10.1007/s11099-008-0066-0

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