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Leaf characteristics and diurnal variation of chlorophyll fluorescence in leaves of the ‘bana’ vegetation of the amazon region

  • Original Papers
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Photosynthetica

Abstract

In six dominant species of the Amazonian ‘Bana’ vegetation, leaf blade characteristics, pigment composition, and chlorophyll (Chl) fluorescence parameters were measured in young and mature leaves under field conditions. Leaf δ13C was comparable in the six species, which suggested that both expanding and expanded leaves contained organic matter fixed under similar intercellular and ambient CO2 concentration (C i/C a). High leaf C/N and negative δ15N values found in this habitat were consistent with the extreme soil N-deficiency. Analysis of Chl and carotenoids showed that expanding leaves had an incomplete development of photosynthetic antenna when compared to adult leaves. Dynamic inactivation of photosystem 2 (PS2) at midday was observed at both leaf ages as Fv/Fm decreased compared to predawn values. Adult leaves reached overnight Fv/Fm ratios typical of healthy leaves. Overnight recovery of Fv/Fm in expanding leaves was incomplete. F0 remained unchanged from midday to predawn and Fv tended to increase from midday to predawn. The recovery from midday depression observed in adult leaves suggested an acclimatory down-regulation associated with photo-protection and non-damage of PS2.

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References

  • Aro, E.-M., Virgin, I., Andersson, N.: Photoinhibition of Photosystem II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113–134, 1993.

    Article  PubMed  CAS  Google Scholar 

  • Bonger, F., Engelen, D., Klinge, H.: Phytomass structure of natural plant communities on spodosol in southern Venezuela: the Bana woodland.-Vegetatio 63: 13–34, 1985.

    Article  Google Scholar 

  • Demmig-Adams B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599–626, 1992.

    Article  CAS  Google Scholar 

  • Dickmann, D.I.: Chlorophyll, ribulose-1,5-diphosphate carboxylase, and Hill reaction activity in developing leaves of Populus deltoides.-Plant Physiol. 48: 143–145, 1971.

    PubMed  CAS  Google Scholar 

  • Ehleringer, J., Osmond, C.B.: Stable isotopes.-In: Pearcy, R.W., Ehleringer, J., Mooney, H.A., Rundel, P.W. (ed.): Plant Physiological Ecology: Field Methods and Instrumentation. Pp. 281–300. Chapman and Hall, New York 1989.

    Google Scholar 

  • Fritschi, F.N., Ray, J.D.: Soybean leaf nitrogen, chlorophyll content, and chlorophyll a/b ratio.-Photosynthetica 45: 92–98, 2007.

    Article  CAS  Google Scholar 

  • Fritz, M., Mueller, C., Matt, P., Feil, R., Stitt, M.: Impact of the C-N status on the amino acid profile in tobacco source leaves.-Plant Cell Environ. 29: 2055–2076, 2006.

    Article  PubMed  CAS  Google Scholar 

  • Grechi, I., Vivin, P., Hilbert, G., Milin, S., Robert, T., Gaudillère, J.P.: Effect of light and nitrogen supply on internal C:N balance and control of root-to-shoot biomass allocation in grapevine.-Environ. exp. Bot. 59: 139–149, 2007.

    Article  CAS  Google Scholar 

  • Greer, D.H., Berry, J.A., Björkman, O.: Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplasts-protein synthesis during recovery.-Planta 168: 253–260, 1986.

    CAS  Google Scholar 

  • Gulmon, S.L., Mooney, H.A.: Costs of defense and their effect on plant productivity.-In: Givnish, T.J. (ed.): On the Economy of Plant Form and Function. Pp. 681–698. Cambridge University Press, Cambridge 1983.

    Google Scholar 

  • Guo, Y.-P., Guo, D.-P., Zhou, H.-F., Hu, M.-J., Shen, Y.-G.: Photoinhibition and xantophyll cycle activity in bayberry (Myrica rubra) leaves induced by high irradiance.-Photosynthetica 44: 439–446, 2006.

    Article  CAS  Google Scholar 

  • Hagg, C., Stober, F., Lichtenthaler, H.K.: Pigment content, chlorophyll fluorescence and photosynthetic activity of spruce clones under normal and limited mineral nutrition.-Photosynthetica 27: 385–400, 1992.

    CAS  Google Scholar 

  • Herrera, R.: Soil and terrain conditions in the International Amazon Project at San Carlos de Rio Negro, Venezuela; correlation with vegetation types.-In: Bruning, E. (ed.): Transac. Internat. MAB-IUFRO Workshop. Pp. 182–188. Hamburg-Reibeck 1977.

  • Herrera, R., Jordan, C.F., Klinge, H., Medina, E.: Amazon ecosystems. Their structure and functioning with particular emphasis on nutrients.-Interciencia 3: 223–232, 1978.

    Google Scholar 

  • Kao, W.-Y., Tsai, H.-C., Shih, C.-N., Tsai, T.-T., Handley, L.L.: Nutrient δ13C and δ15N during leaf senescence in the mangrove Kandelia candel (L.) Druce.-Bot. Bull. Acad. sin. 43: 277–282, 2002.

    CAS  Google Scholar 

  • Kitajima, M., Butler, W.L.: Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone.-Biochim. biophys. Acta 376: 105–115, 1975.

    Article  PubMed  CAS  Google Scholar 

  • Koike, T., Matsuki, S., Choi, D., Matsumoto, T., Sakamoto, Y., Maruyama, Y.: Leaf longevity and defense characteristics in trees of Betulaceae.-Pp. 53–55. Proc. IUFRO, Kanazawa 2003.

  • Krause, G.H., Virgo, A., Winter, K.: High susceptibility to photoinhibition of young leaves of tropical forest trees.-Planta 197: 583–591, 1995.

    Article  CAS  Google Scholar 

  • Krause, G.H., Weis, E.: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals.-Photosynth. Res. 5: 139–157, 1984.

    Article  CAS  Google Scholar 

  • Kursar, T.A., Coley, P.D.: Convergence in defence syndromes of young leaves in tropical rainforests.-Biochem. Syst. Ecol. 31: 929–949, 2003.

    Article  CAS  Google Scholar 

  • Lichtenthaler, H.K., Buschmann, C., Knapp, M.: How to correctly determine the different chlorophyll fluorescence parameters and the chlorophyll decrease ratio RFd of leaves with the PAM fluorometer.-Photosynthetica 43: 379–393, 2005.

    Article  CAS  Google Scholar 

  • Lichtenthaler, H.K., Wellburn, A.R.: Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591–592, 1983.

    Google Scholar 

  • Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633–662, 1994.

    Article  CAS  Google Scholar 

  • Martinelli, L.A., Piccolo, M.C., Townsend, A.R., Vitousek, P.M., Cuevas, E., McDowell, W., Robertson, G.P., Santos, O.C., Treseder, K.: Nitrogen stable isotopic composition of leaves and soil: Tropical versus temperate forests.-Biochemistry 46: 45–65, 1999.

    CAS  Google Scholar 

  • Medina, E., Cuevas, E.: [Nutrient use efficiency in woody plants: Eco-physiology of forests at San Carlos de Rio Negro.]-Sci. Guaianae 11: 51–70, 2000. [In Span.]

    Google Scholar 

  • Medina, E., Minchin, P.: Stratification of δ13C values of leaves in Amazonian rain forests.-Oecologia 45: 377–378, 1980.

    Article  Google Scholar 

  • Pate, J.S., Atkins, C.A.: Xylem and phloem transport and the leaf functional economy of carbon and nitrogen of a legume leaf.-Plant Physiol. 71: 835–840, 1983.

    Article  PubMed  CAS  Google Scholar 

  • Roháček, K.: Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning, and mutual relationships.-Photosynthetica 40: 13–29, 2002.

    Article  Google Scholar 

  • Šantrůček, J., Šiffel, P., Lang, M., Lichtenthaler, H.K., Schindler, C., Synková, H., Konečná, V., Szabo, K.: Photosynthetic activity and chlorophyll fluorescence parameters in aurea and green forms of Nicotiana tabacum.-Photosynthetica 27: 529–543, 1992.

    Google Scholar 

  • Šesták, Z.: Ratio of photosystem 1 and 2 particles in young and old leaves of spinach and radish.-Photosynthetica 3: 285–287, 1969.

    Google Scholar 

  • Šesták, Z.: Chlorophylls and carotenoids during leaf ontogeny.-In: Šesták, Z. (ed.): Photosynthesis during Leaf Development. Pp. 76–106. Academia, Praha; Dr W. Junk Publ., Dordrecht-Boston-Lancaster 1985.

    Google Scholar 

  • Šesták, Z., Šiffel, P.: Leaf-age related differences in chlorophyll fluorescence.-Photosynthetica 33: 347–369, 1997.

    Google Scholar 

  • Sobrado, M.A.: [Ecophysiology of the Sclerophyllous Vegetation Growing in Podzolized Sandy Soils at the Rio Negro Basin in the Amazon Region.]-Thesis in Biology. Faculty of Science, U.C.V., Caracas 1977. [In Span.]

    Google Scholar 

  • Sobrado, M.A.: Leaf photosynthesis and water loss as influenced by leaf age and seasonal drought in an evergreen tree.-Photosynthetica 32: 563–568, 1996.

    Google Scholar 

  • Sobrado, M.A., Medina, E.: General morphology, anatomical structure, and nutrient content of sclerophyllous leaves of the ‘bana’ vegetation of Amazonas.-Oecologia 45: 341–345, 1980.

    Article  Google Scholar 

  • Sokal, R.R., Rohlf, F.J.: Biometry.-W.H. Freeman and Co., San Francisco 1969.

    Google Scholar 

  • Wallace, A.R.: A Narrative of Travels on the Amazon and Rio Negro.-Dover Publications, New York 1889.

    Google Scholar 

  • Weng, J.-H.: Understimate of PS2 efficiency in the field due to high leaf temperature resulting from leaf clipping and its amendment.-Photosynthetica 44: 467–470, 2006.

    Article  Google Scholar 

Download references

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  1. Laboratorio de Biología Ambiental de Plantas, Departamento de Biología de Organismos, Universidad Simón Bolívar, Apartado 89.000, Caracas, 1080 A, Venezuela

    M. A. Sobrado

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  1. M. A. Sobrado
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Sobrado, M.A. Leaf characteristics and diurnal variation of chlorophyll fluorescence in leaves of the ‘bana’ vegetation of the amazon region. Photosynthetica 46, 202–207 (2008). https://doi.org/10.1007/s11099-008-0033-9

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  • DOI: https://doi.org/10.1007/s11099-008-0033-9

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