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Combined Application of Prototype Ultrasound and BSA-Loaded PLGA Particles for Protein Delivery

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A Correction to this article was published on 27 October 2021

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Abstract

Purpose

To develop an in vitro culture system for tissue engineering to mimic the in vivo environment and evaluate the applicability of ultrasound and PLGA particle system.

Methods

For tissue engineering, large molecules such as growth factors for cell differentiation should be supplied in a controlled manner into the culture system, and the in vivo microenvironment need to be reproduced in the system for the regulation of cellular function. In this study, portable prototype ultrasound with low intensity was devised and tested for protein release from bovine serum albumin (BSA)-loaded poly(lactic-co-glycolic acid) (PLGA) particles.

Results

BSA-loaded PLGA particles were prepared using various types of PLGA reagents and their physicochemical properties were characterized including particle size, shape, or aqueous wetting profiles. The BSA-loaded formulation showed nano-ranged size distribution with optimal physical stability during storage period, and protein release behaviors in a controlled manner. Notably, the application of prototype ultrasound with low intensity influenced protein release patterns in the culture system containing the BSA-loaded PLGA formulation. The results revealed that the portable ultrasound set controlled by the computer could contribute for the protein delivery in the culture medium.

Conclusions

This study suggests that combined application with ultrasound and protein-loaded PLGA encapsulation system could be utilized to improve culture system for tissue engineering or cell regeneration therapy.

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Acknowledgments

Authors thank the staff in the public facility at Changwon National University for technical assistance on the instrumental analysis.

Funding

This work was supported by the 2017 Inje University research grant (no. 20180217).

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Author notes

  1. Minki Jin and Sung Ho Seo contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje University, 197 Injero, Gimhae, Gyeongnam, 50834, Republic of Korea

    Minki Jin, Seungmi Hwang, Kwan Hyung Cho, Jimi Byeon, Doyeon Kim & Kyoung Ah Min

  2. Department of Nanoscience and Engineering, School of Biomedical Engineering, Inje University, Gimhae, 50834, Republic of Korea

    Sung Ho Seo, Bo Seok Kim & Changhan Yoon

  3. Department of Biomedical Engineering, Inje University, Gimhae, 50834, Republic of Korea

    Yun Gyeong Kang, Jung-Woog Shin & Changhan Yoon

  4. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, 501 Jinju Daero, Jinju, Gyeongnam, 52828, Republic of Korea

    Meong Cheol Shin

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  1. Minki Jin
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  2. Sung Ho Seo
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Correspondence to Changhan Yoon or Kyoung Ah Min.

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Jin, M., Seo, S.H., Kim, B.S. et al. Combined Application of Prototype Ultrasound and BSA-Loaded PLGA Particles for Protein Delivery. Pharm Res 38, 1455–1466 (2021). https://doi.org/10.1007/s11095-021-03091-z

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  • DOI: https://doi.org/10.1007/s11095-021-03091-z

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