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The Preservation of Lyophilized Human Growth Hormone Activity: how Do Buffers and Sugars Interact?

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Abstract

Purpose

One of the most common classes of excipients used in protein formulations are buffers. The aim of this work is to investigate the effect of buffers on protein stabilization given by sugars during freeze drying.

Methods

Molecular Dynamics simulations of human growth hormone (hGH) in the presence of sucrose and trehalose were performed, and the impact of phosphate and citrate buffers on their protective action was analyzed.

Results

We found that buffers broke the hydrogen bonding network formed by excipients, and the consequences of this disruption of structure ordering were different in sucrose-based or trehalose-based formulations. More specifically, we observed that buffers increased protein recovery in the presence of excipients, such as sucrose, that exert their action mainly by preferential exclusion. By contrast, the opposite effect was sometimes noted in the case of excipients, such as trehalose, whose protective action is related to the formation of a highly structured matrix.

Conclusions

We found that buffers have important properties, other than the control of pH, that can contribute to the overall stability of proteins. Some of these properties are related to their interaction with the other components of the formulation.

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Abbreviations

ATB:

Automated topology builder

COM:

Center of mass

hGH:

Human growth hormone

mAbs:

Monoclonal antibodies

PDB:

Protein data bank

PME:

Particle mesh Ewald

RMSD:

Root mean square deviation

SPC/E:

Extended simple point charge model

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ACKNOWLEDGMENTS AND DISCLOSURES

The authors thank the hpc@polito team, which provided the computational resources for simulations (http://www.hpc.polito.it).

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Authors and Affiliations

  1. Department of Applied Science and Technology, Politecnico di Torino, 24 corso Duca degli Abruzzi, 10129, Torino, Italy

    Andrea Arsiccio & Roberto Pisano

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  1. Andrea Arsiccio
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  2. Roberto Pisano
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Correspondence to Roberto Pisano.

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Arsiccio, A., Pisano, R. The Preservation of Lyophilized Human Growth Hormone Activity: how Do Buffers and Sugars Interact?. Pharm Res 35, 131 (2018). https://doi.org/10.1007/s11095-018-2410-9

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  • DOI: https://doi.org/10.1007/s11095-018-2410-9

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