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Isostatic Ultra-High-Pressure Effects on Supercooled Melts in Colloidal Triglyceride Dispersions

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Purpose

Colloidal triglyceride dispersions can form metastable supercooled melts that need tedious tempering processes to be transformed into a crystalline state. We investigated the possibility of transforming the supercooled melts into crystals in a short time by treatment with isostatic high pressure.

Methods

Nanosized supercooled melts of triglycerides in aqueous dispersions (prepared by high-pressure homogenization) were exposed to isostatic ultrahigh pressure for short periods to initialize crystallization processes. The dispersions were analyzed with different appropriate measuring methods such as differential scanning calorimetry, nuclear magnetic resonance, X-ray scattering, and electron paramagnetic resonance. The resulting particle sizes were estimated by photon correlation spectroscopy and transmission electron microscopy.

Results

The results of differential scanning calorimetry, X-ray, and nuclear magnetic resonance experiments show the induction of triglyceride particle crystallization by high-pressure treatment. Electron paramagnetic resonance spectroscopy shows that the triglyceride crystallization coincides with relocation of the lipophilic probe into a more polar environment. Transmission electron microscopy micrographs show the transformation of supercooled liquid particles into crystallized anisotropic particles.

Conclusion

Crystal transformation in nanoscaled systems can be delayed for months, depending on thematerials and the composite. It is shown that isostatic high-pressure treatment can be a valuable method to induce, accelerate, and control crystallization processes in specific colloidal triglyceride dispersions.

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Abbreviations

HHP:

high hydrostatic pressure

HP:

high pressure

HPH:

high pressure homogenization

IUHP:

isostatic ultrahigh pressure

LCT:

long chain triglycerides

SLN:

solid lipid nanoparticles

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Acknowledgments

The authors cordially thank Dr. Günter Förster (Institute of Physical Chemistry, Martin Luther University Halle) for the X-ray scattering support, Dr. Dieter Ströhl (Institute of Organic Chemistry, Martin Luther University Halle) for the NMR measurements, Dr. Gerd Hause (Biocentre of the Martin Luther University Halle) for the TEM micrograph support, and Mark Freeman (Stansted Fluid Power Ltd.) for the drawings of the HP vessel and the technical support.

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Authors and Affiliations

  1. Department of Pharmacy, Institute of Pharmaceutics and Biopharmaceutics, Martin Luther University Halle-Wittenberg, Wolfgang-Langenbeck-Strasse 4, 06120, Halle/Saale, Germany

    Christoph Blümer & Karsten Mäder

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  1. Christoph Blümer
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  2. Karsten Mäder
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Correspondence to Karsten Mäder.

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Blümer, C., Mäder, K. Isostatic Ultra-High-Pressure Effects on Supercooled Melts in Colloidal Triglyceride Dispersions. Pharm Res 22, 1708–1715 (2005). https://doi.org/10.1007/s11095-005-6949-x

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  • DOI: https://doi.org/10.1007/s11095-005-6949-x

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