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A Method for Assay of Losartan, Its Active Metabolite E-3174, and Glibenclamide in Human Serum and Urine by HPLC-MS/MS

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Pharmaceutical Chemistry Journal Aims and scope

A simple, rapid, and reliable method for simultaneous assay of losartan, its active metabolite E-3174, and glibenclamide was developed and validated. The method is universal and allows metabolites to be assayed in both serum and urea. Application of the method for determination of the activity of isoenzyme CYP2C9 with simultaneous monitoring of glibenclamide conditions was demonstrated. The method was shown to be specific for assay of study compounds in patients simultaneously taking multiple drugs.

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References

  1. M. Zoppi, S. Braunschweig, U. P. Kuenzi, et al., Eur. J. Clin. Pharmacol., 56(5), 427 – 430 (2000).

    Article  CAS  PubMed  Google Scholar 

  2. J. Kirchheiner, J. Brockmöller, I. Meineke, et al., Clin. Pharmacol. Ther., 71(4), 286 – 296 (2002).

    Article  CAS  PubMed  Google Scholar 

  3. A. Surendiran, S. C. Pradhan, A. Agrawal, et al., Eur. J. Clin. Pharmacol., 67(8), 797 – 801 (2011).

    Article  CAS  PubMed  Google Scholar 

  4. Z. Li, G. Wang, L. S. Wang, et al., Xenobiotica, 39(10), 788 – 793 (2009).

    Article  CAS  PubMed  Google Scholar 

  5. A. Petsalo, M. Turpeinen, O. Pelkonen, et al., J. Chromatogr. A, 1215(1 – 2), 107 – 115 (2008).

  6. P. Dorado, E. Machín, F. de Andrés, et al., Drug Metab. Drug Interact., 27(4), 217 – 223 (2012).

    Article  CAS  Google Scholar 

  7. F. De Andrés, M. Sosa-Macías, and A. Lerena, Bioanalysis, 6(5), 683 – 696 (2014).

    Article  PubMed  Google Scholar 

  8. Ü. Yasar, C. Forslund-Bergengren, G. Tybring, et al., Clin. Pharmacol. Ther., 71(1), 89 – 98 (2002).

    Article  CAS  PubMed  Google Scholar 

  9. S. Tanaka, S. Uchida, N. Inui, et al., Biol. Pharm. Bull., 37(1), 18 – 25 (2014).

    Article  CAS  PubMed  Google Scholar 

  10. G. Sartor, A. Melander, B. Schersten, et al., Diabetologia, 18(1), 17–22 (1980).

    Article  CAS  PubMed  Google Scholar 

  11. L. A. Sánchez-Nava, U. Bautista-Sánchez, A. L. Robles-Piedras, GSC Biol. Pharmaceut. Sci., 7(01), 96 – 101 (2019).

    Article  Google Scholar 

  12. G. I. Gorodetskaya, E. V. Shikh, M. V. Zhuravleva, et al., Ved. Nauchn. Tsentr. Éksper. Sredstv. Med. Primen., 7(4), 199 – 211 (2017).

    Google Scholar 

  13. I. E. Shokhin, G. V. Ramenskaya, G. F. Vasilenko, et al., Vopr. Biol. Med. Farm. Khim., 7(6), 36 – 38 (2009).

    Google Scholar 

  14. C. Hess, F. Musshoff, and B. Madea, Anal. Bioanal. Chem., 400(1), 33 – 41 (2011).

    Article  CAS  PubMed  Google Scholar 

  15. K. S. Lakshmi and T. Rajesh, ISRN Pharmaceutics, 2011 (2011).

  16. T. M. Binz, N. Villani, H. Neels, et al., Forensic Sci. Int., 223(1–3), 119 – 124 (2012).

    Article  CAS  PubMed  Google Scholar 

  17. X. Zhang, X. Wang, D. I. Vernikovskaya, et al., Biomed. Chromatogr., 29(4), 560 – 569 (2015).

    Article  CAS  PubMed  Google Scholar 

  18. H. J. Shah, M. L. Kundlik, N. K. Patel, et al., J. Sep. Sci., 32(20), 3388 – 3394 (2009).

    Article  CAS  PubMed  Google Scholar 

  19. B. Prasaja, L. Sasongko, Y. Harahap, et al., J. Pharmaceut. Biomed., 49(3), 862 – 867 (2009).

    Article  CAS  Google Scholar 

  20. M. Polinko, K. Riffel, H. Song, et al., J. Pharmaceut. Biomed., 33(1), 73 – 84 (2003).

    Article  CAS  Google Scholar 

  21. T. Iwasa, T. Takano, K. I. Hara, et al., J. Chromatogr. B, 734(2), 325 – 330 (1999).

    Article  CAS  Google Scholar 

  22. R. A. Stearns, P. K. Chakravarty, R. Chen, et al., Drug Metab. Dispos., 23(2), 207 – 215 (1995).

    CAS  PubMed  Google Scholar 

  23. P. A. Shah, P. Sharma, J. V. Shah, et al., Turk. J. Chem., 39(4), 714 – 733 (2015).

    Article  CAS  Google Scholar 

  24. C. Krämer, J. Sunkomat, J. Witte, et al., Circ. Res., 90(7), 770 – 776 (2002).

    Article  PubMed  Google Scholar 

  25. C. I. Furtek and M. W. Lo, J. Chromatogr. B, 573(2), 295 – 301 (1992).

    Article  CAS  Google Scholar 

  26. T. Rydberg, A. Jönsson, and A. Melander, J. Clin. Pharm. Ther., 20(5), 283 – 295 (1995).

    Article  CAS  PubMed  Google Scholar 

  27. I. Niopas and A. C. Daftsios, J. Pharmaceut. Biomed., 28(3–4), 653 – 657 (2002).

    Article  CAS  Google Scholar 

  28. A. N. Mironov (ed.), Guidelines for Expert Assessment of Medicines [in Russian], Grif i. K, Moscow (2013), Vol. 1, pp. 201 – 207.

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Authors and Affiliations

  1. Scientific Center for Expert Evaluation of Medicinal Products, Russian Federation Ministry of Health, Building 2, 8 Petrovskii Boulevard, 127051, Moscow, Russia

    G. I. Gorodetskaya, N. D. Bunyatyan, E. S. Mel’nikov, T. A. Rodina, S. A. Belkov & L. M. Krasnykh

  2. I. M. Sechenov First Moscow State Medical University, Russian Ministry of Health (Sechenov University), Moscow, Russia, Russian Federation Ministry of Health, Building 2, 8 Trubetskaya Street, 119991, Moscow, Russia

    G. I. Gorodetskaya, N. D. Bunyatyan & E. S. Mel’nikov

Authors
  1. G. I. Gorodetskaya
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  2. N. D. Bunyatyan
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  3. E. S. Mel’nikov
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  4. T. A. Rodina
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  5. S. A. Belkov
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  6. L. M. Krasnykh
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Corresponding author

Correspondence to G. I. Gorodetskaya.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 56, No. 1, pp. 52 – 59, January, 2022.

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Gorodetskaya, G.I., Bunyatyan, N.D., Mel’nikov, E.S. et al. A Method for Assay of Losartan, Its Active Metabolite E-3174, and Glibenclamide in Human Serum and Urine by HPLC-MS/MS. Pharm Chem J 56, 118–125 (2022). https://doi.org/10.1007/s11094-022-02612-3

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  • DOI: https://doi.org/10.1007/s11094-022-02612-3

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